Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
Background: Sclerotinia sclerotiorum is a necrotrophic fungus that causes Sclerotinia head rot (SHR) in sunflower, with epidemics leading to severe yield losses. In this work, we present an association mapping (AM) approach to investigate the genetic basis of natural resistance to SHR in cultivated...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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BMC
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7559 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-020-02529-7 https://doi.org/10.1186/s12870-020-02529-7 |
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| author | Filippi, Carla Valeria Zubrzycki, Jeremias Enrique Di Rienzo, Julio Alejandro Quiroz, Facundo Jose Puebla, Andrea Fabiana Alvarez, Daniel Maringolo, Carla Andrea Escande, Alberto Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana |
| author_browse | Alvarez, Daniel Di Rienzo, Julio Alejandro Escande, Alberto Filippi, Carla Valeria Heinz, Ruth Amelia Hopp, Horacio Esteban Lia, Veronica Viviana Maringolo, Carla Andrea Paniego, Norma Beatriz Puebla, Andrea Fabiana Quiroz, Facundo Jose Zubrzycki, Jeremias Enrique |
| author_facet | Filippi, Carla Valeria Zubrzycki, Jeremias Enrique Di Rienzo, Julio Alejandro Quiroz, Facundo Jose Puebla, Andrea Fabiana Alvarez, Daniel Maringolo, Carla Andrea Escande, Alberto Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana |
| author_sort | Filippi, Carla Valeria |
| collection | INTA Digital |
| description | Background: Sclerotinia sclerotiorum is a necrotrophic fungus that causes Sclerotinia head rot (SHR) in sunflower, with epidemics leading to severe yield losses. In this work, we present an association mapping (AM) approach to investigate the genetic basis of natural resistance to SHR in cultivated sunflower, the fourth most widely grown oilseed crop in the world.
Results: Our association mapping population (AMP), which comprises 135 inbred breeding lines (ILs), was genotyped using 27 candidate genes, a panel of 9 Simple Sequence Repeat (SSR) markers previously associated with SHR resistance via bi-parental mapping, and a set of 384 SNPs located in genes with molecular functions related to stress responses. Moreover, given the complexity of the trait, we evaluated four disease descriptors (i.e, disease incidence, disease severity, area under the disease progress curve for disease incidence, and incubation period). As a result, this work constitutes the most exhaustive AM study of disease resistance in sunflower performed to date. Mixed linear models accounting for population structure and kinship relatedness were used for the statistical analysis of phenotype-genotype associations, allowing the identification of 13 markers associated with disease reduction. The number of favourable alleles was negatively correlated to disease incidence, disease severity and area under the disease progress curve for disease incidence, whereas it was positevily correlated to the incubation period.
Conclusions: Four of the markers identified here as associated with SHR resistance (HA1848, HaCOI_1, G33 and G34) validate previous research, while other four novel markers (SNP117, SNP136, SNP44, SNP128) were consistently associated with SHR resistance, emerging as promising candidates for marker-assisted breeding. From the germplasm point of view, the five ILs carrying the largest combination of resistance alleles provide a valuable resource for sunflower breeding programs worldwide. |
| format | Artículo |
| id | INTA7559 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | BMC |
| publisherStr | BMC |
| record_format | dspace |
| spelling | INTA75592020-07-15T17:39:56Z Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower Filippi, Carla Valeria Zubrzycki, Jeremias Enrique Di Rienzo, Julio Alejandro Quiroz, Facundo Jose Puebla, Andrea Fabiana Alvarez, Daniel Maringolo, Carla Andrea Escande, Alberto Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana Helianthus annuus Enfermedades de las Plantas Sclerotinia Genética Resistencia a la Enfermedad Plant Diseases Genetics Disease Resistance Rots Podredumbres Girasol Sunflower Background: Sclerotinia sclerotiorum is a necrotrophic fungus that causes Sclerotinia head rot (SHR) in sunflower, with epidemics leading to severe yield losses. In this work, we present an association mapping (AM) approach to investigate the genetic basis of natural resistance to SHR in cultivated sunflower, the fourth most widely grown oilseed crop in the world. Results: Our association mapping population (AMP), which comprises 135 inbred breeding lines (ILs), was genotyped using 27 candidate genes, a panel of 9 Simple Sequence Repeat (SSR) markers previously associated with SHR resistance via bi-parental mapping, and a set of 384 SNPs located in genes with molecular functions related to stress responses. Moreover, given the complexity of the trait, we evaluated four disease descriptors (i.e, disease incidence, disease severity, area under the disease progress curve for disease incidence, and incubation period). As a result, this work constitutes the most exhaustive AM study of disease resistance in sunflower performed to date. Mixed linear models accounting for population structure and kinship relatedness were used for the statistical analysis of phenotype-genotype associations, allowing the identification of 13 markers associated with disease reduction. The number of favourable alleles was negatively correlated to disease incidence, disease severity and area under the disease progress curve for disease incidence, whereas it was positevily correlated to the incubation period. Conclusions: Four of the markers identified here as associated with SHR resistance (HA1848, HaCOI_1, G33 and G34) validate previous research, while other four novel markers (SNP117, SNP136, SNP44, SNP128) were consistently associated with SHR resistance, emerging as promising candidates for marker-assisted breeding. From the germplasm point of view, the five ILs carrying the largest combination of resistance alleles provide a valuable resource for sunflower breeding programs worldwide. Instituto de Biotecnología Fil: Filippi, Carla Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zubrzycki, Jeremias Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Biocódices; Argentina Fil: Di Rienzo, Julio Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Estadística y Biometría; Argentina Fil: Quiroz, Facundo Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Patología Vegetal; Argentina Fil: Puebla, Andrea Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Alvarez, Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Maringolo, Carla Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Patología Vegetal; Argentina Fil: Escande, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Patología Vegetal; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina 2020-07-15T17:34:53Z 2020-07-15T17:34:53Z 2020-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7559 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-020-02529-7 1471-2229 https://doi.org/10.1186/s12870-020-02529-7 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC BMC Plant Biology 20 : article number: 322 (2020) |
| spellingShingle | Helianthus annuus Enfermedades de las Plantas Sclerotinia Genética Resistencia a la Enfermedad Plant Diseases Genetics Disease Resistance Rots Podredumbres Girasol Sunflower Filippi, Carla Valeria Zubrzycki, Jeremias Enrique Di Rienzo, Julio Alejandro Quiroz, Facundo Jose Puebla, Andrea Fabiana Alvarez, Daniel Maringolo, Carla Andrea Escande, Alberto Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower |
| title | Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower |
| title_full | Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower |
| title_fullStr | Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower |
| title_full_unstemmed | Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower |
| title_short | Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower |
| title_sort | unveiling the genetic basis of sclerotinia head rot resistance in sunflower |
| topic | Helianthus annuus Enfermedades de las Plantas Sclerotinia Genética Resistencia a la Enfermedad Plant Diseases Genetics Disease Resistance Rots Podredumbres Girasol Sunflower |
| url | http://hdl.handle.net/20.500.12123/7559 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-020-02529-7 https://doi.org/10.1186/s12870-020-02529-7 |
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