Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata
This report deals with the process of improving the stability of medfly, Ceratitis capitata, genetic sexing strains (GSS) based on the swmutation on chromosome 2. This gene affects the rate of development as well as the eye colour and iridescence. The improved sexing strains were produced by mapping...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7529 https://doi.org/10.1023/A:1020963709795 |
| _version_ | 1855484027737210880 |
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| author | Delprat, María Alejandra Stolar, Cristian Eric Manso, Fanny Celia Cladera Jorge Luis |
| author_browse | Cladera Jorge Luis Delprat, María Alejandra Manso, Fanny Celia Stolar, Cristian Eric |
| author_facet | Delprat, María Alejandra Stolar, Cristian Eric Manso, Fanny Celia Cladera Jorge Luis |
| author_sort | Delprat, María Alejandra |
| collection | INTA Digital |
| description | This report deals with the process of improving the stability of medfly, Ceratitis capitata, genetic sexing strains (GSS) based on the swmutation on chromosome 2. This gene affects the rate of development as well as the eye colour and iridescence. The improved sexing strains were produced by mapping swwith deletions and then inducing and screening for new translocations with breakpoints close to the marker. The stability was assessed in large populations over many generations. Twenty-two new Y-2 translocations were identified and polytene chromosome analysis was performed to locate breakpoints. The translocation strains were ranked according to the distance of their breakpoints from sw. The map position of swis region 20D on 2R. As data on the stability of the 22 strains accumulated, Cast191 was shown to be the most promising as no recombination between swand the male sex was found. After rearing the strain for 22 generations under semi-mass rearing conditions, with a population size of 15,000 adults and scoring 1000 flies per generation, only one such event was detected (estimated frequency = 3.1 × 10−6). Further tests are being carried out with this strain to assess its suitability as a genetic sexing strain for medfly Sterile insect technique (SIT). |
| format | Artículo |
| id | INTA7529 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA75292022-10-31T13:04:17Z Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata Delprat, María Alejandra Stolar, Cristian Eric Manso, Fanny Celia Cladera Jorge Luis Ceratitis capitata Plagas de Plantas Estabilidad Genética Recombinación Pests of Plants Genetic Stability Recombination Mosca del Mediterráneo Recombinación Genética This report deals with the process of improving the stability of medfly, Ceratitis capitata, genetic sexing strains (GSS) based on the swmutation on chromosome 2. This gene affects the rate of development as well as the eye colour and iridescence. The improved sexing strains were produced by mapping swwith deletions and then inducing and screening for new translocations with breakpoints close to the marker. The stability was assessed in large populations over many generations. Twenty-two new Y-2 translocations were identified and polytene chromosome analysis was performed to locate breakpoints. The translocation strains were ranked according to the distance of their breakpoints from sw. The map position of swis region 20D on 2R. As data on the stability of the 22 strains accumulated, Cast191 was shown to be the most promising as no recombination between swand the male sex was found. After rearing the strain for 22 generations under semi-mass rearing conditions, with a population size of 15,000 adults and scoring 1000 flies per generation, only one such event was detected (estimated frequency = 3.1 × 10−6). Further tests are being carried out with this strain to assess its suitability as a genetic sexing strain for medfly Sterile insect technique (SIT). Instituto de Genética Fil: Delprat, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Stolar, Cristian Eric. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Manso, Fanny Celia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2020-07-07T17:44:51Z 2020-07-07T17:44:51Z 2002-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7529 0016-6707 1573-6857 https://doi.org/10.1023/A:1020963709795 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Genetica 116 : 85–95 (2002) |
| spellingShingle | Ceratitis capitata Plagas de Plantas Estabilidad Genética Recombinación Pests of Plants Genetic Stability Recombination Mosca del Mediterráneo Recombinación Genética Delprat, María Alejandra Stolar, Cristian Eric Manso, Fanny Celia Cladera Jorge Luis Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata |
| title | Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata |
| title_full | Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata |
| title_fullStr | Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata |
| title_full_unstemmed | Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata |
| title_short | Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata |
| title_sort | genetic stability of sexing strains based on the locus swof ceratitis capitata |
| topic | Ceratitis capitata Plagas de Plantas Estabilidad Genética Recombinación Pests of Plants Genetic Stability Recombination Mosca del Mediterráneo Recombinación Genética |
| url | http://hdl.handle.net/20.500.12123/7529 https://doi.org/10.1023/A:1020963709795 |
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