A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit
In tomato, numerous wild-related species have been demonstrated to be untapped sources of valuable genetic variability, including pathogen-resistance genes, nutritional, and industrial quality traits. From a collection of S. pennellii introgressed lines, 889 fruit metabolic loci (QML) and 326 yield-...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Society for Experimental Biology
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7493 https://academic.oup.com/jxb/article/59/10/2875/442281 https://doi.org/10.1093/jxb/ern146 |
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| author | Bermúdez, Luisa Urias, Ursula Milstein, D. Kamenetzky, Laura Asís, Ramón Fernie, Alisdair R. Van Sluys, M.A. Carrari, Fernando Rossi, Magdalena |
| author_browse | Asís, Ramón Bermúdez, Luisa Carrari, Fernando Fernie, Alisdair R. Kamenetzky, Laura Milstein, D. Rossi, Magdalena Urias, Ursula Van Sluys, M.A. |
| author_facet | Bermúdez, Luisa Urias, Ursula Milstein, D. Kamenetzky, Laura Asís, Ramón Fernie, Alisdair R. Van Sluys, M.A. Carrari, Fernando Rossi, Magdalena |
| author_sort | Bermúdez, Luisa |
| collection | INTA Digital |
| description | In tomato, numerous wild-related species have been demonstrated to be untapped sources of valuable genetic variability, including pathogen-resistance genes, nutritional, and industrial quality traits. From a collection of S. pennellii introgressed lines, 889 fruit metabolic loci (QML) and 326 yield-associated loci (YAL), distributed across the tomato genome, had been identified previously. By using a combination of molecular marker sequence analysis, PCR amplification and sequencing, analysis of allelic variation, and evaluation of co-response between gene expression and metabolite composition traits, the present report, provides a comprehensive list of candidate genes co-localizing with a subset of 106 QML and 20 YAL associated either with important agronomic or nutritional characteristics. This combined strategy allowed the identification and analysis of 127 candidate genes located in 16 regions of the tomato genome. Eighty-five genes were cloned and partially sequenced, totalling 45 816 and 45 787 bases from S. lycopersicum and S. pennellii, respectively. Allelic variation at the amino acid level was confirmed for 37 of these candidates. Furthermore, out of the 127 gene-metabolite co-locations, some 56 were recovered following correlation of parallel transcript and metabolite profiling. Results obtained here represent the initial steps in the integration of genetic, genomic, and expressional patterns of genes co-localizing with chemical compositional traits of the tomato fruit. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7493 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Society for Experimental Biology |
| publisherStr | Society for Experimental Biology |
| record_format | dspace |
| spelling | INTA74932020-06-30T14:54:14Z A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit Bermúdez, Luisa Urias, Ursula Milstein, D. Kamenetzky, Laura Asís, Ramón Fernie, Alisdair R. Van Sluys, M.A. Carrari, Fernando Rossi, Magdalena Tomate Loci de Rasgos Cuantitativos Variación Genética Marcadores Genéticos Tomatoes Quantitative Trait Loci Genetic Variation Genetic Markers QTL (quantitative trait loci) Marcadores Moleculares In tomato, numerous wild-related species have been demonstrated to be untapped sources of valuable genetic variability, including pathogen-resistance genes, nutritional, and industrial quality traits. From a collection of S. pennellii introgressed lines, 889 fruit metabolic loci (QML) and 326 yield-associated loci (YAL), distributed across the tomato genome, had been identified previously. By using a combination of molecular marker sequence analysis, PCR amplification and sequencing, analysis of allelic variation, and evaluation of co-response between gene expression and metabolite composition traits, the present report, provides a comprehensive list of candidate genes co-localizing with a subset of 106 QML and 20 YAL associated either with important agronomic or nutritional characteristics. This combined strategy allowed the identification and analysis of 127 candidate genes located in 16 regions of the tomato genome. Eighty-five genes were cloned and partially sequenced, totalling 45 816 and 45 787 bases from S. lycopersicum and S. pennellii, respectively. Allelic variation at the amino acid level was confirmed for 37 of these candidates. Furthermore, out of the 127 gene-metabolite co-locations, some 56 were recovered following correlation of parallel transcript and metabolite profiling. Results obtained here represent the initial steps in the integration of genetic, genomic, and expressional patterns of genes co-localizing with chemical compositional traits of the tomato fruit. Instituto de Biotecnología Fil: Bermúdez, Luisa. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Urias, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Milstein, D. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Kamenetzky, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Fernie, Alisdair R. Max Planck Institute for Molecular Plant Physiology; Alemania Fil: Van Sluys, M.A. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Rossi, Magdalena. Universidade de São Pablo. Instituto de Biociências. Departamento de Botânica; Brasil 2020-06-30T14:39:05Z 2020-06-30T14:39:05Z 2008-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7493 https://academic.oup.com/jxb/article/59/10/2875/442281 0022-0957 1460-2431 https://doi.org/10.1093/jxb/ern146 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Society for Experimental Biology Journal of Experimental Botany 59 (10) : 2875–2890 (July 2008) |
| spellingShingle | Tomate Loci de Rasgos Cuantitativos Variación Genética Marcadores Genéticos Tomatoes Quantitative Trait Loci Genetic Variation Genetic Markers QTL (quantitative trait loci) Marcadores Moleculares Bermúdez, Luisa Urias, Ursula Milstein, D. Kamenetzky, Laura Asís, Ramón Fernie, Alisdair R. Van Sluys, M.A. Carrari, Fernando Rossi, Magdalena A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit |
| title | A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit |
| title_full | A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit |
| title_fullStr | A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit |
| title_full_unstemmed | A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit |
| title_short | A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit |
| title_sort | candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit |
| topic | Tomate Loci de Rasgos Cuantitativos Variación Genética Marcadores Genéticos Tomatoes Quantitative Trait Loci Genetic Variation Genetic Markers QTL (quantitative trait loci) Marcadores Moleculares |
| url | http://hdl.handle.net/20.500.12123/7493 https://academic.oup.com/jxb/article/59/10/2875/442281 https://doi.org/10.1093/jxb/ern146 |
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