Genetic dissection of vitamin E biosynthesis in tomato
Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for α-t...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Society for Experimental Biology
2020
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/7492 https://academic.oup.com/jxb/article/62/11/3781/501890 https://doi.org/10.1093/jxb/err055 |
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| author | Almeida de Souza, Juliana Beatriz Quadrana, Leandro Daniel Asis, Ramón Setta, Nathalia Godoy, Fabiana de Bermúdez, Luisa Otaiza, Santiago N. Silva, Junia V. Corrêa da Fernie, Alisdair R. Carrari, Fernando Rossi, Magdalena |
| author_browse | Almeida de Souza, Juliana Beatriz Asis, Ramón Bermúdez, Luisa Carrari, Fernando Fernie, Alisdair R. Godoy, Fabiana de Otaiza, Santiago N. Quadrana, Leandro Daniel Rossi, Magdalena Setta, Nathalia Silva, Junia V. Corrêa da |
| author_facet | Almeida de Souza, Juliana Beatriz Quadrana, Leandro Daniel Asis, Ramón Setta, Nathalia Godoy, Fabiana de Bermúdez, Luisa Otaiza, Santiago N. Silva, Junia V. Corrêa da Fernie, Alisdair R. Carrari, Fernando Rossi, Magdalena |
| author_sort | Almeida de Souza, Juliana Beatriz |
| collection | INTA Digital |
| description | Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for α-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (α, β, γ, and δ) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7492 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Society for Experimental Biology |
| publisherStr | Society for Experimental Biology |
| record_format | dspace |
| spelling | INTA74922020-06-30T14:22:01Z Genetic dissection of vitamin E biosynthesis in tomato Almeida de Souza, Juliana Beatriz Quadrana, Leandro Daniel Asis, Ramón Setta, Nathalia Godoy, Fabiana de Bermúdez, Luisa Otaiza, Santiago N. Silva, Junia V. Corrêa da Fernie, Alisdair R. Carrari, Fernando Rossi, Magdalena Tomate Genética Tocoferoles Vitamina E Loci de Rasgos Cuantitativos Tomatoes Genetics Tocopherols Vitamin E Quantitative Trait Loci QTL (quantitative trait loci) Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for α-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (α, β, γ, and δ) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical. Instituto de Biotecnología Fil: Almeida de Souza, Juliana Beatriz. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Setta, Nathalia. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Godoy, Fabiana de. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Bermúdez, Luisa. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Otaiza, Santiago N. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Silva, Junia V. Corrêa da. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Fernie, Alisdair R. Max Planck Institute for Molecular Plant Physiology; Alemania Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Rossi, Magdalena. Universidade de São Pablo. Instituto de Biociências. Departamento de Botânica; Brasil 2020-06-30T14:02:12Z 2020-06-30T14:02:12Z 2011-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7492 https://academic.oup.com/jxb/article/62/11/3781/501890 0022-0957 1460-2431 https://doi.org/10.1093/jxb/err055 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Society for Experimental Biology Journal of Experimental Botany 62 (11) : 3781–3798. (July 2011) |
| spellingShingle | Tomate Genética Tocoferoles Vitamina E Loci de Rasgos Cuantitativos Tomatoes Genetics Tocopherols Vitamin E Quantitative Trait Loci QTL (quantitative trait loci) Almeida de Souza, Juliana Beatriz Quadrana, Leandro Daniel Asis, Ramón Setta, Nathalia Godoy, Fabiana de Bermúdez, Luisa Otaiza, Santiago N. Silva, Junia V. Corrêa da Fernie, Alisdair R. Carrari, Fernando Rossi, Magdalena Genetic dissection of vitamin E biosynthesis in tomato |
| title | Genetic dissection of vitamin E biosynthesis in tomato |
| title_full | Genetic dissection of vitamin E biosynthesis in tomato |
| title_fullStr | Genetic dissection of vitamin E biosynthesis in tomato |
| title_full_unstemmed | Genetic dissection of vitamin E biosynthesis in tomato |
| title_short | Genetic dissection of vitamin E biosynthesis in tomato |
| title_sort | genetic dissection of vitamin e biosynthesis in tomato |
| topic | Tomate Genética Tocoferoles Vitamina E Loci de Rasgos Cuantitativos Tomatoes Genetics Tocopherols Vitamin E Quantitative Trait Loci QTL (quantitative trait loci) |
| url | http://hdl.handle.net/20.500.12123/7492 https://academic.oup.com/jxb/article/62/11/3781/501890 https://doi.org/10.1093/jxb/err055 |
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