Supramolecular solvent-based high-throughput sample treatment for monitoring phytohormones in plant tissues
Quantification of endogenous hormones in plants is essential to understand their growth, development and response to biotic and abiotic stresses. However, it is challenging to develop high-throughput sample treatments from complex plant tissues containing low amounts of structurally unrelated and la...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7470 https://www.sciencedirect.com/science/article/abs/pii/S0039914020305403 https://doi.org/10.1016/j.talanta.2020.121249 |
| _version_ | 1855484016062365696 |
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| author | Yonny, Melisa Evangelina Ballesteros-Gómez, Ana Toscano Adamo, Maria L. Rodriguez Torressi, Ariel Nazareno, Mónica Azucena Rubio, Soledad |
| author_browse | Ballesteros-Gómez, Ana Nazareno, Mónica Azucena Rodriguez Torressi, Ariel Rubio, Soledad Toscano Adamo, Maria L. Yonny, Melisa Evangelina |
| author_facet | Yonny, Melisa Evangelina Ballesteros-Gómez, Ana Toscano Adamo, Maria L. Rodriguez Torressi, Ariel Nazareno, Mónica Azucena Rubio, Soledad |
| author_sort | Yonny, Melisa Evangelina |
| collection | INTA Digital |
| description | Quantification of endogenous hormones in plants is essential to understand their growth, development and response to biotic and abiotic stresses. However, it is challenging to develop high-throughput sample treatments from complex plant tissues containing low amounts of structurally unrelated and labile phytohormones while delivering clean and analyte-enriched extracts. In this paper we propose the use of supramolecular solvents (SUPRASs) made up or inverted hexagonal nanostructures of alkanols to address this challenge. The strategy was applied, as a proof of concept, to the quantification of stress-related phytohormones belonging to different categories (abscisic acid, salicylic acid, jasmonic acid, methyl jasmonate and 3-indoleacetic acid) in melon and pepper leaves. Sample treatment consisted in a single extraction-cleanup step involving the use of a low volume of SUPRAS (244 μL), the stirring (5 min) and centrifugation (15 min) of the sample at room temperature, and the direct analysis of the extract by liquid chromatography tandem mass spectrometry (LC-MS/MS). This high-throughput sample treatment method delivered excellent results for the target phytohormones regarding absolute recoveries (80–92%), method quantification limits (0.05–2 ng g−1), reproducibility (1–7%) and matrix effects (+13 to −31%), in both melon and pepper leaves, compared to reported methods based on repetitive solvent extraction, purification and solvent evaporation steps. The method was successfully applied to determine target hormones in melon and pepper plants for the evaluation of the effect of thermal stress. It was found that their concentration increased in the ranges 1.2–1.9 and 1.3–3.8 times in melon and pepper leaves, respectively, compared with control samples. |
| format | Artículo |
| id | INTA7470 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| record_format | dspace |
| spelling | INTA74702022-03-29T12:14:50Z Supramolecular solvent-based high-throughput sample treatment for monitoring phytohormones in plant tissues Yonny, Melisa Evangelina Ballesteros-Gómez, Ana Toscano Adamo, Maria L. Rodriguez Torressi, Ariel Nazareno, Mónica Azucena Rubio, Soledad Sustancias de Crecimiento Vegetal Solventes Estrés Térmico Tejidos Vegetales Plant Growth Substances Solvents Heat Stress Plant Tissues Fitohormonas Phytohormones Quantification of endogenous hormones in plants is essential to understand their growth, development and response to biotic and abiotic stresses. However, it is challenging to develop high-throughput sample treatments from complex plant tissues containing low amounts of structurally unrelated and labile phytohormones while delivering clean and analyte-enriched extracts. In this paper we propose the use of supramolecular solvents (SUPRASs) made up or inverted hexagonal nanostructures of alkanols to address this challenge. The strategy was applied, as a proof of concept, to the quantification of stress-related phytohormones belonging to different categories (abscisic acid, salicylic acid, jasmonic acid, methyl jasmonate and 3-indoleacetic acid) in melon and pepper leaves. Sample treatment consisted in a single extraction-cleanup step involving the use of a low volume of SUPRAS (244 μL), the stirring (5 min) and centrifugation (15 min) of the sample at room temperature, and the direct analysis of the extract by liquid chromatography tandem mass spectrometry (LC-MS/MS). This high-throughput sample treatment method delivered excellent results for the target phytohormones regarding absolute recoveries (80–92%), method quantification limits (0.05–2 ng g−1), reproducibility (1–7%) and matrix effects (+13 to −31%), in both melon and pepper leaves, compared to reported methods based on repetitive solvent extraction, purification and solvent evaporation steps. The method was successfully applied to determine target hormones in melon and pepper plants for the evaluation of the effect of thermal stress. It was found that their concentration increased in the ranges 1.2–1.9 and 1.3–3.8 times in melon and pepper leaves, respectively, compared with control samples. EEA Santiago del Estero Fil: Yonny, Melisa. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Laboratorio de Antioxidantes y Procesos Oxidativos; Argentina Fil: Ballesteros-Gómez, Ana. Universidad de Córdoba. Departamento de Química Analítica. Instituto de Química Fina y Nanoquímica; España Fil: Toscano Adamo, Maria L. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Laboratorio de Antioxidantes y Procesos Oxidativos; Argentina Fil: Rodriguez Torressi, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina Fil: Nazareno, Mónica A. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Laboratorio de Antioxidantes y Procesos Oxidativos; Argentina Fil: Rubio, Soledad. Universidad de Córdoba. Departamento de Química Analítica. Instituto de Química Fina y Nanoquímica; España 2020-06-25T14:20:50Z 2020-06-25T14:20:50Z 2020-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7470 https://www.sciencedirect.com/science/article/abs/pii/S0039914020305403 0039-9140 https://doi.org/10.1016/j.talanta.2020.121249 eng info:eu-repo/semantics/restrictedAccess application/pdf Talanta Volume 219 : 121249 (November 2020) |
| spellingShingle | Sustancias de Crecimiento Vegetal Solventes Estrés Térmico Tejidos Vegetales Plant Growth Substances Solvents Heat Stress Plant Tissues Fitohormonas Phytohormones Yonny, Melisa Evangelina Ballesteros-Gómez, Ana Toscano Adamo, Maria L. Rodriguez Torressi, Ariel Nazareno, Mónica Azucena Rubio, Soledad Supramolecular solvent-based high-throughput sample treatment for monitoring phytohormones in plant tissues |
| title | Supramolecular solvent-based high-throughput sample treatment for monitoring phytohormones in plant tissues |
| title_full | Supramolecular solvent-based high-throughput sample treatment for monitoring phytohormones in plant tissues |
| title_fullStr | Supramolecular solvent-based high-throughput sample treatment for monitoring phytohormones in plant tissues |
| title_full_unstemmed | Supramolecular solvent-based high-throughput sample treatment for monitoring phytohormones in plant tissues |
| title_short | Supramolecular solvent-based high-throughput sample treatment for monitoring phytohormones in plant tissues |
| title_sort | supramolecular solvent based high throughput sample treatment for monitoring phytohormones in plant tissues |
| topic | Sustancias de Crecimiento Vegetal Solventes Estrés Térmico Tejidos Vegetales Plant Growth Substances Solvents Heat Stress Plant Tissues Fitohormonas Phytohormones |
| url | http://hdl.handle.net/20.500.12123/7470 https://www.sciencedirect.com/science/article/abs/pii/S0039914020305403 https://doi.org/10.1016/j.talanta.2020.121249 |
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