First report of group A rotavirus and bovine coronavirus associated with neonatal calf diarrhea in the northwest of Argentina
Group A rotavirus (RVA) and bovine coronavirus (BCoV) are the two main viral enteropathogens associated with neonatal calf diarrhea. The aim of the present survey was to investigate the epidemiology and the role of RVA and BCoV in the presentation of dairy and beef calf diarrhea in Lerma Valley of S...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7469 https://link.springer.com/article/10.1007%2Fs11250-020-02293-8 https://doi.org/10.1007/s11250-020-02293-8 |
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| author | Bertoni, Emiliano Agustín Aduriz Guerrero, Matías Bok, Marina Vega, Celina Guadalupe Saif, Linda J. Aguirre, Daniel Hector Cimino, Rubén Oscar Miño, Samuel Parreño, Gladys Viviana |
| author_browse | Aduriz Guerrero, Matías Aguirre, Daniel Hector Bertoni, Emiliano Agustín Bok, Marina Cimino, Rubén Oscar Miño, Samuel Parreño, Gladys Viviana Saif, Linda J. Vega, Celina Guadalupe |
| author_facet | Bertoni, Emiliano Agustín Aduriz Guerrero, Matías Bok, Marina Vega, Celina Guadalupe Saif, Linda J. Aguirre, Daniel Hector Cimino, Rubén Oscar Miño, Samuel Parreño, Gladys Viviana |
| author_sort | Bertoni, Emiliano Agustín |
| collection | INTA Digital |
| description | Group A rotavirus (RVA) and bovine coronavirus (BCoV) are the two main viral enteropathogens associated with neonatal calf diarrhea. The aim of the present survey was to investigate the epidemiology and the role of RVA and BCoV in the presentation of dairy and beef calf diarrhea in Lerma Valley of Salta province, within the Northwest region of Argentina. Stool samples of calves with or without diarrhea younger than 2 months of age were collected from 19 dairy farms and 20 beef farms between the years 2014 and 2016. Stool samples were screened for RVA and BCoV detection by ELISA. Heminested multiplex RT-PCR was used for RVA typing and RT-PCR to confirm BCoV. Positive samples were submitted to sequencing analysis. Bovine RVA and BCoV were circulating in 63% (12/19) and 10.52% (2/19) of the dairy farms, respectively, where 9.5% (46/484) of the calves were positives to RVA and 0.4% (2/484) to BCoV. In beef herds, RVA was detected in 40% (8/20) of the farms and in 6.75% (21/311) of the calves, without positives cases of BCoV. Molecular analysis showed that in dairy farms, G6P[11] and G10P[11] were the prevalent RVA strains, while in beef farms, G10P[11] was the prevalent. The main finding was the detection for the first time of a G15P[11] causing diarrhea in beef calves of Argentina that represents a new alert to be consider for future vaccine updates. Analysis of detected BCoV showed that it is related to the other circulating strains of Argentina. |
| format | Artículo |
| id | INTA7469 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA74692020-06-24T20:04:57Z First report of group A rotavirus and bovine coronavirus associated with neonatal calf diarrhea in the northwest of Argentina Bertoni, Emiliano Agustín Aduriz Guerrero, Matías Bok, Marina Vega, Celina Guadalupe Saif, Linda J. Aguirre, Daniel Hector Cimino, Rubén Oscar Miño, Samuel Parreño, Gladys Viviana Enfermedades de los Animales Ganado Bovino Rota Virus Diarrea de Terneros Rotavirus Coronaviridae Argentina Animal Diseases Calf Diarrhoea Rotavirus Cattle Región Noroeste, Argentina Group A rotavirus (RVA) and bovine coronavirus (BCoV) are the two main viral enteropathogens associated with neonatal calf diarrhea. The aim of the present survey was to investigate the epidemiology and the role of RVA and BCoV in the presentation of dairy and beef calf diarrhea in Lerma Valley of Salta province, within the Northwest region of Argentina. Stool samples of calves with or without diarrhea younger than 2 months of age were collected from 19 dairy farms and 20 beef farms between the years 2014 and 2016. Stool samples were screened for RVA and BCoV detection by ELISA. Heminested multiplex RT-PCR was used for RVA typing and RT-PCR to confirm BCoV. Positive samples were submitted to sequencing analysis. Bovine RVA and BCoV were circulating in 63% (12/19) and 10.52% (2/19) of the dairy farms, respectively, where 9.5% (46/484) of the calves were positives to RVA and 0.4% (2/484) to BCoV. In beef herds, RVA was detected in 40% (8/20) of the farms and in 6.75% (21/311) of the calves, without positives cases of BCoV. Molecular analysis showed that in dairy farms, G6P[11] and G10P[11] were the prevalent RVA strains, while in beef farms, G10P[11] was the prevalent. The main finding was the detection for the first time of a G15P[11] causing diarrhea in beef calves of Argentina that represents a new alert to be consider for future vaccine updates. Analysis of detected BCoV showed that it is related to the other circulating strains of Argentina. Instituto de Investigación Animal del Chaco Semiárido Fil: Bertoni, Emiliano Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; Argentina Fil: Aduriz Guerrero, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Saif, LInda J. Ohio State University. Food Animal Health Research Program; Estados Unidos Fil: Aguirre, Daniel Hector. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; Argentina Fil: Cimino, Rubén Oscar. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Parreño, Gladis Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-06-24T19:59:18Z 2020-06-24T19:59:18Z 2020-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7469 https://link.springer.com/article/10.1007%2Fs11250-020-02293-8 0049-4747 1573-7438 https://doi.org/10.1007/s11250-020-02293-8 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Tropical Animal Health and Production (2020) |
| spellingShingle | Enfermedades de los Animales Ganado Bovino Rota Virus Diarrea de Terneros Rotavirus Coronaviridae Argentina Animal Diseases Calf Diarrhoea Rotavirus Cattle Región Noroeste, Argentina Bertoni, Emiliano Agustín Aduriz Guerrero, Matías Bok, Marina Vega, Celina Guadalupe Saif, Linda J. Aguirre, Daniel Hector Cimino, Rubén Oscar Miño, Samuel Parreño, Gladys Viviana First report of group A rotavirus and bovine coronavirus associated with neonatal calf diarrhea in the northwest of Argentina |
| title | First report of group A rotavirus and bovine coronavirus associated with neonatal calf diarrhea in the northwest of Argentina |
| title_full | First report of group A rotavirus and bovine coronavirus associated with neonatal calf diarrhea in the northwest of Argentina |
| title_fullStr | First report of group A rotavirus and bovine coronavirus associated with neonatal calf diarrhea in the northwest of Argentina |
| title_full_unstemmed | First report of group A rotavirus and bovine coronavirus associated with neonatal calf diarrhea in the northwest of Argentina |
| title_short | First report of group A rotavirus and bovine coronavirus associated with neonatal calf diarrhea in the northwest of Argentina |
| title_sort | first report of group a rotavirus and bovine coronavirus associated with neonatal calf diarrhea in the northwest of argentina |
| topic | Enfermedades de los Animales Ganado Bovino Rota Virus Diarrea de Terneros Rotavirus Coronaviridae Argentina Animal Diseases Calf Diarrhoea Rotavirus Cattle Región Noroeste, Argentina |
| url | http://hdl.handle.net/20.500.12123/7469 https://link.springer.com/article/10.1007%2Fs11250-020-02293-8 https://doi.org/10.1007/s11250-020-02293-8 |
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