Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongs...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Annex Publishers
2020
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| Online Access: | http://www.annexpublishers.com/articles/JVSAH/7204-Development-and-Validation-of-a-Novel-ELISA-for-the-Detection-of-Neospora-Caninum-Antibodies-in-Bovine-Sera.pdf http://hdl.handle.net/20.500.12123/7466 |
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| author | Mansilla, Florencia Celeste Gual, Ignacio Pereyra, Walter Rodrigo Cardoso, Nancy Patricia Turco, Cecilia Capozzo, Alejandra Victoria |
| author_browse | Capozzo, Alejandra Victoria Cardoso, Nancy Patricia Gual, Ignacio Mansilla, Florencia Celeste Pereyra, Walter Rodrigo Turco, Cecilia |
| author_facet | Mansilla, Florencia Celeste Gual, Ignacio Pereyra, Walter Rodrigo Cardoso, Nancy Patricia Turco, Cecilia Capozzo, Alejandra Victoria |
| author_sort | Mansilla, Florencia Celeste |
| collection | INTA Digital |
| description | Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA has become the most eligible method for large-scale screening of specific antibodies against N. caninum in diagnostic laboratories around the world, providing results that can be easily standardized between laboratories, within a few hours at a relative low-cost. There is no local production of commercial ELISA tests nor in Argentina neither in South America, therefore they must be purchased abroad, turning unfeasible the routinely use of ELISA tests. Here we have developed and validated accordingly to OIE specifications a novel ELISA test based on soluble antigens from N. caninum tachyzoites to accurately determine the presence of specific antibodies anti- N. caninum antigens in bovine sera samples, enabling the rapid, objective and low-cost diagnosis of bovine neosporosis in Argentina; replacing IFAT and avoiding delays and high importation taxes. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7466 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Annex Publishers |
| publisherStr | Annex Publishers |
| record_format | dspace |
| spelling | INTA74662020-06-24T15:35:51Z Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera Mansilla, Florencia Celeste Gual, Ignacio Pereyra, Walter Rodrigo Cardoso, Nancy Patricia Turco, Cecilia Capozzo, Alejandra Victoria Técnicas Inmunológicas ELISA Bovinae Neospora Caninum Anticuerpos Immunological Techniques Bovina Antibodies IgG Bovine Sera Samples Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA has become the most eligible method for large-scale screening of specific antibodies against N. caninum in diagnostic laboratories around the world, providing results that can be easily standardized between laboratories, within a few hours at a relative low-cost. There is no local production of commercial ELISA tests nor in Argentina neither in South America, therefore they must be purchased abroad, turning unfeasible the routinely use of ELISA tests. Here we have developed and validated accordingly to OIE specifications a novel ELISA test based on soluble antigens from N. caninum tachyzoites to accurately determine the presence of specific antibodies anti- N. caninum antigens in bovine sera samples, enabling the rapid, objective and low-cost diagnosis of bovine neosporosis in Argentina; replacing IFAT and avoiding delays and high importation taxes. EEA Balcarce Fil: Mansilla Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Fil: Gual Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pereyra Walter Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cardoso Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Turco Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Fil: Capozzo Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-06-24T15:24:58Z 2020-06-24T15:24:58Z 2019-08-26 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://www.annexpublishers.com/articles/JVSAH/7204-Development-and-Validation-of-a-Novel-ELISA-for-the-Detection-of-Neospora-Caninum-Antibodies-in-Bovine-Sera.pdf http://hdl.handle.net/20.500.12123/7466 2348-9790 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Annex Publishers Journal of veterinary science and animal husbandry 7 (2) : 204. (August 29, 2019) |
| spellingShingle | Técnicas Inmunológicas ELISA Bovinae Neospora Caninum Anticuerpos Immunological Techniques Bovina Antibodies IgG Bovine Sera Samples Mansilla, Florencia Celeste Gual, Ignacio Pereyra, Walter Rodrigo Cardoso, Nancy Patricia Turco, Cecilia Capozzo, Alejandra Victoria Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera |
| title | Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera |
| title_full | Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera |
| title_fullStr | Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera |
| title_full_unstemmed | Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera |
| title_short | Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera |
| title_sort | development and validation of a novel elisa for the detection of neospora caninum antibodies in bovine sera |
| topic | Técnicas Inmunológicas ELISA Bovinae Neospora Caninum Anticuerpos Immunological Techniques Bovina Antibodies IgG Bovine Sera Samples |
| url | http://www.annexpublishers.com/articles/JVSAH/7204-Development-and-Validation-of-a-Novel-ELISA-for-the-Detection-of-Neospora-Caninum-Antibodies-in-Bovine-Sera.pdf http://hdl.handle.net/20.500.12123/7466 |
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