Involvement of nitrate reductase (NR) in osmotic stress-induced NO generation of Arabidopsis thaliana L. roots
Nitric oxide (NO) is undoubtedly a potential signal molecule in diverse developmental processes and stress responses. Despite our extensive knowledge about the role of NO in physiological and stress responses, the source of this gaseous molecule is still unresolved. The aim of this study was to inve...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7457 https://www.sciencedirect.com/science/article/pii/S0176161709003861 https://doi.org/10.1016/j.jplph.2009.08.013 |
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| author | Kolbert, Zsuzsanna Ortega, Leandro Ismael Erdei, László |
| author_browse | Erdei, László Kolbert, Zsuzsanna Ortega, Leandro Ismael |
| author_facet | Kolbert, Zsuzsanna Ortega, Leandro Ismael Erdei, László |
| author_sort | Kolbert, Zsuzsanna |
| collection | INTA Digital |
| description | Nitric oxide (NO) is undoubtedly a potential signal molecule in diverse developmental processes and stress responses. Despite our extensive knowledge about the role of NO in physiological and stress responses, the source of this gaseous molecule is still unresolved. The aim of this study was to investigate the potential role of nitrate reductase (NR) as the source of NO accumulation in the root system of wild-type and NR-deficient nia1, nia2 mutant Arabidopsis plants under osmotic stress conditions induced by a polyethylene glycol (PEG 6000) treatment. Reduction of primary root (PR) length was detected as the effect of osmotic stress in wild-type and NR-deficient plants. We found that osmotic stress-induced lateral root (LR) initiation in wild-type, but not in NR-mutant plants. High levels of NO formation occurred in roots of Col-1 plants as the effect of PEG treatment. The mammalian nitric oxide synthase (NOS) inhibitor NG-monomethyl-l-arginine (l-NMMA) had no effect on LR initiation or NO generation, while tungstate, an NR inhibitor, inhibited the later phase of osmotic stress-induced NO accumulation and slightly decreased the LR development. In nia1, nia2 roots, the PEG treatment induced the first phase of NO production, but later NO production was inhibited. We conclude that the first phase of PEG-induced NO generation is not dependent on NOS-like or NR activity. It is also suggested that the activity of NR in roots is required for the later phase of osmotic stress-induced NO formation. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7457 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA74572020-06-23T13:01:59Z Involvement of nitrate reductase (NR) in osmotic stress-induced NO generation of Arabidopsis thaliana L. roots Kolbert, Zsuzsanna Ortega, Leandro Ismael Erdei, László Estrés Osmótico Arabidopsis thaliana Nitrato Reductasa Óxido Nítrico Osmotic Stress Nitrate Reductase Nitric Oxide Estrés Salino Nitric oxide (NO) is undoubtedly a potential signal molecule in diverse developmental processes and stress responses. Despite our extensive knowledge about the role of NO in physiological and stress responses, the source of this gaseous molecule is still unresolved. The aim of this study was to investigate the potential role of nitrate reductase (NR) as the source of NO accumulation in the root system of wild-type and NR-deficient nia1, nia2 mutant Arabidopsis plants under osmotic stress conditions induced by a polyethylene glycol (PEG 6000) treatment. Reduction of primary root (PR) length was detected as the effect of osmotic stress in wild-type and NR-deficient plants. We found that osmotic stress-induced lateral root (LR) initiation in wild-type, but not in NR-mutant plants. High levels of NO formation occurred in roots of Col-1 plants as the effect of PEG treatment. The mammalian nitric oxide synthase (NOS) inhibitor NG-monomethyl-l-arginine (l-NMMA) had no effect on LR initiation or NO generation, while tungstate, an NR inhibitor, inhibited the later phase of osmotic stress-induced NO accumulation and slightly decreased the LR development. In nia1, nia2 roots, the PEG treatment induced the first phase of NO production, but later NO production was inhibited. We conclude that the first phase of PEG-induced NO generation is not dependent on NOS-like or NR activity. It is also suggested that the activity of NR in roots is required for the later phase of osmotic stress-induced NO formation. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Kolbert, Zsuzsanna. University of Szeged. Faculty of Science and Informatics. Department of Plant Biology; Hungría Fil: Ortega, Leandro Ismael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales (ex IFFIVE); Argentina Fil: Erdei, László. University of Szeged. Faculty of Science and Informatics. Department of Plant Biology; Hungría 2020-06-23T12:56:32Z 2020-06-23T12:56:32Z 2010-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7457 https://www.sciencedirect.com/science/article/pii/S0176161709003861 0176-1617 https://doi.org/10.1016/j.jplph.2009.08.013 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of Plant Physiology 167 (1) : 77-80 (January 2010) |
| spellingShingle | Estrés Osmótico Arabidopsis thaliana Nitrato Reductasa Óxido Nítrico Osmotic Stress Nitrate Reductase Nitric Oxide Estrés Salino Kolbert, Zsuzsanna Ortega, Leandro Ismael Erdei, László Involvement of nitrate reductase (NR) in osmotic stress-induced NO generation of Arabidopsis thaliana L. roots |
| title | Involvement of nitrate reductase (NR) in osmotic stress-induced NO generation of Arabidopsis thaliana L. roots |
| title_full | Involvement of nitrate reductase (NR) in osmotic stress-induced NO generation of Arabidopsis thaliana L. roots |
| title_fullStr | Involvement of nitrate reductase (NR) in osmotic stress-induced NO generation of Arabidopsis thaliana L. roots |
| title_full_unstemmed | Involvement of nitrate reductase (NR) in osmotic stress-induced NO generation of Arabidopsis thaliana L. roots |
| title_short | Involvement of nitrate reductase (NR) in osmotic stress-induced NO generation of Arabidopsis thaliana L. roots |
| title_sort | involvement of nitrate reductase nr in osmotic stress induced no generation of arabidopsis thaliana l roots |
| topic | Estrés Osmótico Arabidopsis thaliana Nitrato Reductasa Óxido Nítrico Osmotic Stress Nitrate Reductase Nitric Oxide Estrés Salino |
| url | http://hdl.handle.net/20.500.12123/7457 https://www.sciencedirect.com/science/article/pii/S0176161709003861 https://doi.org/10.1016/j.jplph.2009.08.013 |
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