Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat

Plants co-ordinate information derived from many diverse external and internal signals to ensure appropriate control of gene expression under optimal and stress conditions. In this work, the relationships between catalase (CAT) and H2O2 during drought in wheat (Triticum aestivum L.) are studied. Dro...

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Autores principales: Luna, Celina Mercedes, Pastori, Gabriela M., Driscoll, Simon, Groten, Karin, Bernard, Stephanie, Foyer, Christine H.
Formato: Artículo
Lenguaje:Inglés
Publicado: Society for Experimental Biology 2020
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/7448
https://academic.oup.com/jxb/article/56/411/417/429881
https://doi.org/10.1093/jxb/eri039
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author Luna, Celina Mercedes
Pastori, Gabriela M.
Driscoll, Simon
Groten, Karin
Bernard, Stephanie
Foyer, Christine H.
author_browse Bernard, Stephanie
Driscoll, Simon
Foyer, Christine H.
Groten, Karin
Luna, Celina Mercedes
Pastori, Gabriela M.
author_facet Luna, Celina Mercedes
Pastori, Gabriela M.
Driscoll, Simon
Groten, Karin
Bernard, Stephanie
Foyer, Christine H.
author_sort Luna, Celina Mercedes
collection INTA Digital
description Plants co-ordinate information derived from many diverse external and internal signals to ensure appropriate control of gene expression under optimal and stress conditions. In this work, the relationships between catalase (CAT) and H2O2 during drought in wheat (Triticum aestivum L.) are studied. Drought-induced H2O2 accumulation correlated with decreases in soil water content and CO2 assimilation. Leaf H2O2 content increased even though total CAT activity doubled under severe drought conditions. Diurnal regulation of CAT1 and CAT2 mRNA abundance was apparent in all conditions and day/night CAT1 and CAT2 expression patterns were modified by mild and severe drought. The abundance of CAT1 transcripts was regulated by circadian controls that persisted in continuous darkness, while CAT2 was modulated by light. Drought decreased abundance, and modified the pattern, of CAT1 and CAT2 mRNAs. It was concluded that the complex regulation of CAT mRNA, particularly at the level of translation, allows precise control of leaf H2O2 accumulation.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2020
publishDateRange 2020
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publisher Society for Experimental Biology
publisherStr Society for Experimental Biology
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spelling INTA74482020-06-22T12:30:39Z Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat Luna, Celina Mercedes Pastori, Gabriela M. Driscoll, Simon Groten, Karin Bernard, Stephanie Foyer, Christine H. Trigo Sequía Catalasa Expresión Génica Peróxido de Hidrógeno Wheat Drought Catalase Gene Expression Hydrogen Peroxide Plants co-ordinate information derived from many diverse external and internal signals to ensure appropriate control of gene expression under optimal and stress conditions. In this work, the relationships between catalase (CAT) and H2O2 during drought in wheat (Triticum aestivum L.) are studied. Drought-induced H2O2 accumulation correlated with decreases in soil water content and CO2 assimilation. Leaf H2O2 content increased even though total CAT activity doubled under severe drought conditions. Diurnal regulation of CAT1 and CAT2 mRNA abundance was apparent in all conditions and day/night CAT1 and CAT2 expression patterns were modified by mild and severe drought. The abundance of CAT1 transcripts was regulated by circadian controls that persisted in continuous darkness, while CAT2 was modulated by light. Drought decreased abundance, and modified the pattern, of CAT1 and CAT2 mRNAs. It was concluded that the complex regulation of CAT mRNA, particularly at the level of translation, allows precise control of leaf H2O2 accumulation. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Luna, Celina Mercedes. Rothamsted Research. Crop Performance and Improvement Division; Gran Bretaña. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales (ex IFFIVE); Argentina Fil: Pastori, Gabriela M. Rothamsted Research. Crop Performance and Improvement Division; Gran Bretaña Fil: Driscoll, Simon. Rothamsted Research. Crop Performance and Improvement Division; Gran Bretaña Fil: Groten, Karin. Rothamsted Research. Crop Performance and Improvement Division; Gran Bretaña Fil: Bernard, Stephanie. Rothamsted Research. Crop Performance and Improvement Division; Gran Bretaña Fil: Foyer, Christine H. Rothamsted Research. Crop Performance and Improvement Division; Gran Bretaña 2020-06-22T12:26:48Z 2020-06-22T12:26:48Z 2005-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7448 https://academic.oup.com/jxb/article/56/411/417/429881 0022-0957 1460-2431 https://doi.org/10.1093/jxb/eri039 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Society for Experimental Biology Journal of Experimental Botany 56 (411) : 417–423. (January 2005)
spellingShingle Trigo
Sequía
Catalasa
Expresión Génica
Peróxido de Hidrógeno
Wheat
Drought
Catalase
Gene Expression
Hydrogen Peroxide
Luna, Celina Mercedes
Pastori, Gabriela M.
Driscoll, Simon
Groten, Karin
Bernard, Stephanie
Foyer, Christine H.
Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat
title Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat
title_full Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat
title_fullStr Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat
title_full_unstemmed Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat
title_short Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat
title_sort drought controls on h2o2 accumulation catalase cat activity and cat gene expression in wheat
topic Trigo
Sequía
Catalasa
Expresión Génica
Peróxido de Hidrógeno
Wheat
Drought
Catalase
Gene Expression
Hydrogen Peroxide
url http://hdl.handle.net/20.500.12123/7448
https://academic.oup.com/jxb/article/56/411/417/429881
https://doi.org/10.1093/jxb/eri039
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