Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions?
Root hair curling is an early and essential morphological change required for the success of the symbiotic interaction between legumes and rhizobia. At this stage rhizobia grow as an infection thread within root hairs and are internalized into the plant cells by endocytosis, where the PI3K enzyme pl...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Society for Experimental Biology
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7447 https://academic.oup.com/jxb/article/69/8/2037/4831116 https://doi.org/10.1093/jxb/ery030 |
| _version_ | 1855035860382121984 |
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| author | Robert, German Muñoz, Nacira Belen Alvarado-Affantranger, Xochitl Saavedra, Laura Davidenco, Vanina Rodríguez-Kessler, Margarita Estrada-Navarrete, Georgina Sánchez, Federico Lascano, Hernán Ramiro |
| author_browse | Alvarado-Affantranger, Xochitl Davidenco, Vanina Estrada-Navarrete, Georgina Lascano, Hernán Ramiro Muñoz, Nacira Belen Robert, German Rodríguez-Kessler, Margarita Saavedra, Laura Sánchez, Federico |
| author_facet | Robert, German Muñoz, Nacira Belen Alvarado-Affantranger, Xochitl Saavedra, Laura Davidenco, Vanina Rodríguez-Kessler, Margarita Estrada-Navarrete, Georgina Sánchez, Federico Lascano, Hernán Ramiro |
| author_sort | Robert, German |
| collection | INTA Digital |
| description | Root hair curling is an early and essential morphological change required for the success of the symbiotic interaction between legumes and rhizobia. At this stage rhizobia grow as an infection thread within root hairs and are internalized into the plant cells by endocytosis, where the PI3K enzyme plays important roles. Previous observations show that stress conditions affect early stages of the symbiotic interaction, from 2 to 30 min post-inoculation, which we term as very early host responses, and affect symbiosis establishment. Herein, we demonstrated the relevance of the very early host responses for the symbiotic interaction. PI3K and the NADPH oxidase complex are found to have key roles in the microsymbiont recognition response, modulating the apoplastic and intracellular/endosomal ROS induction in root hairs. Interestingly, compared with soybean mutant plants that do not perceive the symbiont, we demonstrated that the very early symbiont perception under sublethal saline stress conditions induced root hair death. Together, these results highlight not only the importance of the very early host-responses on later stages of the symbiont interaction, but also suggest that they act as a mechanism for local control of nodulation capacity, prior to the abortion of the infection thread, preventing the allocation of resources/energy for nodule formation under unfavorable environmental conditions. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7447 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Society for Experimental Biology |
| publisherStr | Society for Experimental Biology |
| record_format | dspace |
| spelling | INTA74472020-06-22T11:49:38Z Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions? Robert, German Muñoz, Nacira Belen Alvarado-Affantranger, Xochitl Saavedra, Laura Davidenco, Vanina Rodríguez-Kessler, Margarita Estrada-Navarrete, Georgina Sánchez, Federico Lascano, Hernán Ramiro Estrés Abiótico Nodulación Fosfatidilinositoles Simbiontico Abiotic Stress Root Nodulation Phosphatidylinositols Symbionts Root hair curling is an early and essential morphological change required for the success of the symbiotic interaction between legumes and rhizobia. At this stage rhizobia grow as an infection thread within root hairs and are internalized into the plant cells by endocytosis, where the PI3K enzyme plays important roles. Previous observations show that stress conditions affect early stages of the symbiotic interaction, from 2 to 30 min post-inoculation, which we term as very early host responses, and affect symbiosis establishment. Herein, we demonstrated the relevance of the very early host responses for the symbiotic interaction. PI3K and the NADPH oxidase complex are found to have key roles in the microsymbiont recognition response, modulating the apoplastic and intracellular/endosomal ROS induction in root hairs. Interestingly, compared with soybean mutant plants that do not perceive the symbiont, we demonstrated that the very early symbiont perception under sublethal saline stress conditions induced root hair death. Together, these results highlight not only the importance of the very early host-responses on later stages of the symbiont interaction, but also suggest that they act as a mechanism for local control of nodulation capacity, prior to the abortion of the infection thread, preventing the allocation of resources/energy for nodule formation under unfavorable environmental conditions. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Alvarado-Affantranger, Xochitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Saavedra, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Davidenco, Vanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Rodríguez-Kessler, Margarita. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Estrada-Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina 2020-06-22T11:44:48Z 2020-06-22T11:44:48Z 2018-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7447 https://academic.oup.com/jxb/article/69/8/2037/4831116 0022-0957 1460-2431 https://doi.org/10.1093/jxb/ery030 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Society for Experimental Biology Journal of Experimental Botany 69 (8) : 2037–2048. (April 2018) |
| spellingShingle | Estrés Abiótico Nodulación Fosfatidilinositoles Simbiontico Abiotic Stress Root Nodulation Phosphatidylinositols Symbionts Robert, German Muñoz, Nacira Belen Alvarado-Affantranger, Xochitl Saavedra, Laura Davidenco, Vanina Rodríguez-Kessler, Margarita Estrada-Navarrete, Georgina Sánchez, Federico Lascano, Hernán Ramiro Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions? |
| title | Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions? |
| title_full | Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions? |
| title_fullStr | Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions? |
| title_full_unstemmed | Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions? |
| title_short | Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions? |
| title_sort | phosphatidylinositol 3 kinase function at very early symbiont perception a local nodulation control under stress conditions |
| topic | Estrés Abiótico Nodulación Fosfatidilinositoles Simbiontico Abiotic Stress Root Nodulation Phosphatidylinositols Symbionts |
| url | http://hdl.handle.net/20.500.12123/7447 https://academic.oup.com/jxb/article/69/8/2037/4831116 https://doi.org/10.1093/jxb/ery030 |
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