DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality
The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) on embryo development and quality. Bovine oocytes were matured with different...
| Main Authors: | , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7380 https://www.sciencedirect.com/science/article/abs/pii/S0093691X20301576 https://doi.org/10.1016/j.theriogenology.2020.02.045 |
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| author | Ynsaurralde Rivolta, Amanda Eugenia Suvá, Mariana Luchetti, Carolina Griselda Bevacqua, Romina Jimena Munilla, Sebastian Rodriguez-Alvarez, Lleretny Velasquez, Alejandra Briski, Olinda Lombardo, Daniel Salamone, Daniel Felipe |
| author_browse | Bevacqua, Romina Jimena Briski, Olinda Lombardo, Daniel Luchetti, Carolina Griselda Munilla, Sebastian Rodriguez-Alvarez, Lleretny Salamone, Daniel Felipe Suvá, Mariana Velasquez, Alejandra Ynsaurralde Rivolta, Amanda Eugenia |
| author_facet | Ynsaurralde Rivolta, Amanda Eugenia Suvá, Mariana Luchetti, Carolina Griselda Bevacqua, Romina Jimena Munilla, Sebastian Rodriguez-Alvarez, Lleretny Velasquez, Alejandra Briski, Olinda Lombardo, Daniel Salamone, Daniel Felipe |
| author_sort | Ynsaurralde Rivolta, Amanda Eugenia |
| collection | INTA Digital |
| description | The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) on embryo development and quality. Bovine oocytes were matured with different DMSO concentrations (0, 0.1, 0.25, 0.5, 0.75, 1 and 10% v:v) followed by in vitro fertilization. Subsequently, quality indicators such as gene expression of SOX2, OCT4, CDX2, SOD1, oocyte and embryo redox status and DNA damage were evaluated. Polar body extrusion and blastocyst rates increased with 0.5% v:v DMSO. Moreover, first polar body extrusion and blastocyst rates did not increase with 1%, and 10% of DMSO reduced polar body extrusion and did not produce blastocyst. Optimal concentration of DMSO for the use on the maturation was estimated at around 0.45% v:v. Supplementation with 0.5% v:v DMSO has not affected mRNA abundance of genes key in blastocyst, however 0.75% increased gene expression of OCT4 and SOX2. Oocytes matured with 0.5% v:v DMSO and blastocyst from DMSO group showed reduced lipid peroxidation respect control. Total Glutathione concentrations increased in blastocyst stage in DMSO group. DMSO increased the total cell number of blastocysts but not TUNEL positive cells. In conclusion, our results suggest that low DMSO concentrations used during bovine oocytes in vitro maturation increases the maturation, as well as the blastocyst rate and its quality, without demonstrating deleterious effect on embryo cells. |
| format | Artículo |
| id | INTA7380 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA73802024-06-07T11:34:37Z DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality Ynsaurralde Rivolta, Amanda Eugenia Suvá, Mariana Luchetti, Carolina Griselda Bevacqua, Romina Jimena Munilla, Sebastian Rodriguez-Alvarez, Lleretny Velasquez, Alejandra Briski, Olinda Lombardo, Daniel Salamone, Daniel Felipe Ganado Bovino Suplementos Estrés Oxidativo Óvulo Experimentación in Vitro Cattle Supplements Oxidative Stress Ova In Vitro Experimentation Oocytes The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) on embryo development and quality. Bovine oocytes were matured with different DMSO concentrations (0, 0.1, 0.25, 0.5, 0.75, 1 and 10% v:v) followed by in vitro fertilization. Subsequently, quality indicators such as gene expression of SOX2, OCT4, CDX2, SOD1, oocyte and embryo redox status and DNA damage were evaluated. Polar body extrusion and blastocyst rates increased with 0.5% v:v DMSO. Moreover, first polar body extrusion and blastocyst rates did not increase with 1%, and 10% of DMSO reduced polar body extrusion and did not produce blastocyst. Optimal concentration of DMSO for the use on the maturation was estimated at around 0.45% v:v. Supplementation with 0.5% v:v DMSO has not affected mRNA abundance of genes key in blastocyst, however 0.75% increased gene expression of OCT4 and SOX2. Oocytes matured with 0.5% v:v DMSO and blastocyst from DMSO group showed reduced lipid peroxidation respect control. Total Glutathione concentrations increased in blastocyst stage in DMSO group. DMSO increased the total cell number of blastocysts but not TUNEL positive cells. In conclusion, our results suggest that low DMSO concentrations used during bovine oocytes in vitro maturation increases the maturation, as well as the blastocyst rate and its quality, without demonstrating deleterious effect on embryo cells. EEA Mercedes Fil: Ynsaurralde Rivolta, Amada Eugenia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Suvá, Mariana. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Luchetti, Carolina Griselda. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Histología y Embriología, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bevacqua, Romina Jimena. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Munilla, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentina Fil: Rodriguez-Alvarez, Lleretny. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile Fil: Velasquez, Alejandra. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile Fil: Briski, Olinda. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lombardo, Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Histología y Embriología, Argentina. Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-06-09T13:21:44Z 2020-06-09T13:21:44Z 2020-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7380 https://www.sciencedirect.com/science/article/abs/pii/S0093691X20301576 0093-691X 1879-3231 https://doi.org/10.1016/j.theriogenology.2020.02.045 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Theriogenology 148 : 140-148 (May 2020) |
| spellingShingle | Ganado Bovino Suplementos Estrés Oxidativo Óvulo Experimentación in Vitro Cattle Supplements Oxidative Stress Ova In Vitro Experimentation Oocytes Ynsaurralde Rivolta, Amanda Eugenia Suvá, Mariana Luchetti, Carolina Griselda Bevacqua, Romina Jimena Munilla, Sebastian Rodriguez-Alvarez, Lleretny Velasquez, Alejandra Briski, Olinda Lombardo, Daniel Salamone, Daniel Felipe DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality |
| title | DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality |
| title_full | DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality |
| title_fullStr | DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality |
| title_full_unstemmed | DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality |
| title_short | DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality |
| title_sort | dmso supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality |
| topic | Ganado Bovino Suplementos Estrés Oxidativo Óvulo Experimentación in Vitro Cattle Supplements Oxidative Stress Ova In Vitro Experimentation Oocytes |
| url | http://hdl.handle.net/20.500.12123/7380 https://www.sciencedirect.com/science/article/abs/pii/S0093691X20301576 https://doi.org/10.1016/j.theriogenology.2020.02.045 |
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