SSR markers linked to stem canker resistance in soybean Glycine max
In this work, we studied 40 samples of Diaporthe phaseolorum var. meridionalis (Dpm), causal agent of stem canker in soybeans (SCS). In the susceptible genotype Golondrina65 the isolate RSF12 showed the highest percentage of the dead plant index (DP = 85.7 %) and was used to characterize all known s...
| Main Authors: | , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo
2020
|
| Subjects: | |
| Online Access: | http://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2933 http://hdl.handle.net/20.500.12123/7333 |
| _version_ | 1855035838924062720 |
|---|---|
| author | Gilli, Javier Ramon Vellicce, Gabriel Ricardo Bernardi, Clarisa Noelia |
| author_browse | Bernardi, Clarisa Noelia Gilli, Javier Ramon Vellicce, Gabriel Ricardo |
| author_facet | Gilli, Javier Ramon Vellicce, Gabriel Ricardo Bernardi, Clarisa Noelia |
| author_sort | Gilli, Javier Ramon |
| collection | INTA Digital |
| description | In this work, we studied 40 samples of Diaporthe phaseolorum var. meridionalis (Dpm), causal agent of stem canker in soybeans (SCS). In the susceptible genotype Golondrina65 the isolate RSF12 showed the highest percentage of the dead plant index (DP = 85.7 %) and was used to characterize all known sources of resistance to Dpm. The soybean MJ19RR experimental line showed the best behaviour against this isolate with a DP = 2.4 % and was used to develop a segregating population with the suscep¬tible cultivar FT-2001. In the F2 generation, the chi-square test determined a 3:1 ratio of resistant plants against susceptible plants, as expected for a dominant gene. In order to advance in our study we propose, as objective, to locate in the soybean genetic map the resistance to Diaporthe phaseolorum var. meridionalis. The Bulked Segregant Analyses and the genetic linkage study identified a region of chromosome 6 of the genetic map of soybean, located at 13.3 cM from the Satt433 locus associated with resistance to SCS. The soybean experimental line MJ19RR was selected as the best source of resistance, and it is available in the active bank of soybean germplasm of INTA, for the genetic control of this disease. The results obtained in this work represent a first approximation for the understanding of the genetic basis of resistance to SCS. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7333 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo |
| publisherStr | Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo |
| record_format | dspace |
| spelling | INTA73332020-06-29T19:38:05Z SSR markers linked to stem canker resistance in soybean Glycine max Gilli, Javier Ramon Vellicce, Gabriel Ricardo Bernardi, Clarisa Noelia Marcadores Genéticos Soja Glycine max Diaporthe phaseolorum Resistencia a la Enfermedad Genetic Markers Soybeans Disease Resistance Marcadores Moleculares In this work, we studied 40 samples of Diaporthe phaseolorum var. meridionalis (Dpm), causal agent of stem canker in soybeans (SCS). In the susceptible genotype Golondrina65 the isolate RSF12 showed the highest percentage of the dead plant index (DP = 85.7 %) and was used to characterize all known sources of resistance to Dpm. The soybean MJ19RR experimental line showed the best behaviour against this isolate with a DP = 2.4 % and was used to develop a segregating population with the suscep¬tible cultivar FT-2001. In the F2 generation, the chi-square test determined a 3:1 ratio of resistant plants against susceptible plants, as expected for a dominant gene. In order to advance in our study we propose, as objective, to locate in the soybean genetic map the resistance to Diaporthe phaseolorum var. meridionalis. The Bulked Segregant Analyses and the genetic linkage study identified a region of chromosome 6 of the genetic map of soybean, located at 13.3 cM from the Satt433 locus associated with resistance to SCS. The soybean experimental line MJ19RR was selected as the best source of resistance, and it is available in the active bank of soybean germplasm of INTA, for the genetic control of this disease. The results obtained in this work represent a first approximation for the understanding of the genetic basis of resistance to SCS. En este trabajo estudiamos 40 muestras de Diaporthe phaseolorum var. meridionalis(Dpm), agente causal del cancro del tallo en soja (CTS). En el control susceptible Golon-drina65, el aislado RSF12 presentó el mayor porcentaje del índice de plantas muertas (DP = 85,7 %) y fue utilizado para caracterizar las fuentes de resistencia conocidas al Dpm. La línea experimental de soja MJ19RR mostró el mejor comportamiento frente a este aislado, con un valor de DP= 2,4 %, y fue utilizada para desarrollar una población segre-gante con el cultivar susceptible FT-2001. En la generación F2 la prueba de chi-cuadrado determinó una proporción 3:1 de plantas resistentes versus plantas susceptibles, como se espera para un gen dominante. Para avanzar en nuestro estudio, proponemos como objetivo localizar en el mapa genético de soja la resistencia a Diaporthe phaseolorum var. meridionalis. El Bulked Segregant Analysesy el estudio de ligamiento genético identifi-caron una región del cromosoma 6 del mapa genético de soja, a 13,3 cM del locus Satt433, asociada a la resistencia al CTS. Además la línea experimental de soja MJ19RR fue selec-cionada como la mejor fuente de resistencia disponible en el banco activo de germo-plasma de soja de INTA para el control genético de esta enfermedad. Los resultados obtenidos en este trabajo representan una primera aproximación para la comprensión de las bases genéticas de la resistencia al CTS. EEA Marcos Juárez Fil: Gilli, Javier Ramon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Vellicce, Gabriel Ricardo. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina; Argentina Fil: Bernardi, Clarisa Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina 2020-05-29T15:04:04Z 2020-05-29T15:04:04Z 2020 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2933 http://hdl.handle.net/20.500.12123/7333 1853-8665 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo Revista de la Facultad de Ciencias Agrarias / Universidad Nacional de Cuyo 52 (1) : 26-39 (2020) |
| spellingShingle | Marcadores Genéticos Soja Glycine max Diaporthe phaseolorum Resistencia a la Enfermedad Genetic Markers Soybeans Disease Resistance Marcadores Moleculares Gilli, Javier Ramon Vellicce, Gabriel Ricardo Bernardi, Clarisa Noelia SSR markers linked to stem canker resistance in soybean Glycine max |
| title | SSR markers linked to stem canker resistance in soybean Glycine max |
| title_full | SSR markers linked to stem canker resistance in soybean Glycine max |
| title_fullStr | SSR markers linked to stem canker resistance in soybean Glycine max |
| title_full_unstemmed | SSR markers linked to stem canker resistance in soybean Glycine max |
| title_short | SSR markers linked to stem canker resistance in soybean Glycine max |
| title_sort | ssr markers linked to stem canker resistance in soybean glycine max |
| topic | Marcadores Genéticos Soja Glycine max Diaporthe phaseolorum Resistencia a la Enfermedad Genetic Markers Soybeans Disease Resistance Marcadores Moleculares |
| url | http://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2933 http://hdl.handle.net/20.500.12123/7333 |
| work_keys_str_mv | AT gillijavierramon ssrmarkerslinkedtostemcankerresistanceinsoybeanglycinemax AT velliccegabrielricardo ssrmarkerslinkedtostemcankerresistanceinsoybeanglycinemax AT bernardiclarisanoelia ssrmarkerslinkedtostemcankerresistanceinsoybeanglycinemax |