In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus.
Aspergillus flavus is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once arvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7283 https://www.mdpi.com/2223-747/9/4/438 https://doi.org/10.3390/plants9040438 |
| _version_ | 1855483976452407296 |
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| author | Di Ciaccio, Lucía Soledad Catalano, Alejandro V. López, Paula G. Rojas, Dante Emanuel Cristos, Diego Sebastian Fortunato, Renee Hersilia Salvat, Adriana Elisabeth |
| author_browse | Catalano, Alejandro V. Cristos, Diego Sebastian Di Ciaccio, Lucía Soledad Fortunato, Renee Hersilia López, Paula G. Rojas, Dante Emanuel Salvat, Adriana Elisabeth |
| author_facet | Di Ciaccio, Lucía Soledad Catalano, Alejandro V. López, Paula G. Rojas, Dante Emanuel Cristos, Diego Sebastian Fortunato, Renee Hersilia Salvat, Adriana Elisabeth |
| author_sort | Di Ciaccio, Lucía Soledad |
| collection | INTA Digital |
| description | Aspergillus flavus is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once arvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of Peltophorum dubium, a species from northern Argentina
(Oriental Chaco), against A. flavus. The antifungal activities of di erent collection sites are reported.
The extracts exhibited a minimum inhibitory concentration of 125 g/mL, and the di erences between the treatments and the inoculum control were 11 mm of P. dubium A and 10 mm of P. dubium F in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions.
The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and aidzein. |
| format | Artículo |
| id | INTA7283 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA72832020-05-21T11:57:40Z In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus. Di Ciaccio, Lucía Soledad Catalano, Alejandro V. López, Paula G. Rojas, Dante Emanuel Cristos, Diego Sebastian Fortunato, Renee Hersilia Salvat, Adriana Elisabeth Aspergillus flavus Flavonoides Flavonoids Plant Bioactivity Evans Blue Active Extract Characterization Bioactividad de la Planta Evans Azul Caracterización de Extracto Activo Aspergillus flavus is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once arvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of Peltophorum dubium, a species from northern Argentina (Oriental Chaco), against A. flavus. The antifungal activities of di erent collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 g/mL, and the di erences between the treatments and the inoculum control were 11 mm of P. dubium A and 10 mm of P. dubium F in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and aidzein. Fil: Di Ciaccio, Lucía Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Catalano, Alejandra V. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Farmacognosia; Argentina Fil: López, Paula G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Farmacognosia; Argentina Fil: Rojas, Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina Fil: Fortunato, Renee Hersilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Salvat, Adriana Elisabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Cristos, Diego Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina 2020-05-21T11:37:33Z 2020-05-21T11:37:33Z 2020-04-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7283 https://www.mdpi.com/2223-747/9/4/438 2223-7747 https://doi.org/10.3390/plants9040438 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Plants 9 (4) : 438 (2020) |
| spellingShingle | Aspergillus flavus Flavonoides Flavonoids Plant Bioactivity Evans Blue Active Extract Characterization Bioactividad de la Planta Evans Azul Caracterización de Extracto Activo Di Ciaccio, Lucía Soledad Catalano, Alejandro V. López, Paula G. Rojas, Dante Emanuel Cristos, Diego Sebastian Fortunato, Renee Hersilia Salvat, Adriana Elisabeth In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus. |
| title | In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus. |
| title_full | In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus. |
| title_fullStr | In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus. |
| title_full_unstemmed | In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus. |
| title_short | In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus. |
| title_sort | in vitro antifungal activity of peltophorum dubium spreg taub extracts against aspergillus flavus |
| topic | Aspergillus flavus Flavonoides Flavonoids Plant Bioactivity Evans Blue Active Extract Characterization Bioactividad de la Planta Evans Azul Caracterización de Extracto Activo |
| url | http://hdl.handle.net/20.500.12123/7283 https://www.mdpi.com/2223-747/9/4/438 https://doi.org/10.3390/plants9040438 |
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