Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita
Genotypes of Lycopersicon peruvianum (L.) Mill. and L. peruvianum var. glandulosum (Rick), selected from accessions that possess resistance to Meloidogyne incognita [(Kofoid and White) Chitwood] at high soil temperature (30C), were used as male parents in crosses with L. esculentum (Mill.) susceptib...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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American Society for Horticultural Science
2020
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| Materias: | |
| Acceso en línea: | https://journals.ashs.org/jashs/view/journals/jashs/116/6/article-p1082.xml http://hdl.handle.net/20.500.12123/7184 https://doi.org/10.21273/JASHS.116.6.1082 |
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| author | Cap, Guillermo Bartolome Roberts, P.A. Thomason, I.J. Murashige, T. |
| author_browse | Cap, Guillermo Bartolome Murashige, T. Roberts, P.A. Thomason, I.J. |
| author_facet | Cap, Guillermo Bartolome Roberts, P.A. Thomason, I.J. Murashige, T. |
| author_sort | Cap, Guillermo Bartolome |
| collection | INTA Digital |
| description | Genotypes of Lycopersicon peruvianum (L.) Mill. and L. peruvianum var. glandulosum (Rick), selected from accessions that possess resistance to Meloidogyne incognita [(Kofoid and White) Chitwood] at high soil temperature (30C), were used as male parents in crosses with L. esculentum (Mill.) susceptible cultivars UC82, Lukullus, Tropic, and male-sterile line ms-31, respectively. The incongruity barrier between the two plant species was overcome by embryo callus and embryo cloning techniques. Hybridity of the F, progeny obtained from each cross was confirmed by differences in leaf and flower morphology, plant growth habits, and by acid phosphatase isozyme phenotypes using polyacrylamide gel electrophoresis. In greenhouse inoculation experiments, F1 plants were highly resistant to M. incognita in soil at 25 and 30C. These results confirmed the successful transfer and expression of heat-stable resistance to M. incognita from L. peruvianum to hybrids with L. esculentum as a preliminary step to introgressing additional root-knot nematode resistance into tomato. |
| format | Artículo |
| id | INTA7184 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | American Society for Horticultural Science |
| publisherStr | American Society for Horticultural Science |
| record_format | dspace |
| spelling | INTA71842020-05-04T13:39:58Z Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita Cap, Guillermo Bartolome Roberts, P.A. Thomason, I.J. Murashige, T. Tomate Solanum peruvianum Híbridos Cultivo de Embriones Genotipos Meloidogyne incognita Resistencia a la Enfermedad Tomatoes Hybrids Embryo Culture Genotypes Disease Resistance Lycopersicon esculentum Lycopersicon peruvianum Genotypes of Lycopersicon peruvianum (L.) Mill. and L. peruvianum var. glandulosum (Rick), selected from accessions that possess resistance to Meloidogyne incognita [(Kofoid and White) Chitwood] at high soil temperature (30C), were used as male parents in crosses with L. esculentum (Mill.) susceptible cultivars UC82, Lukullus, Tropic, and male-sterile line ms-31, respectively. The incongruity barrier between the two plant species was overcome by embryo callus and embryo cloning techniques. Hybridity of the F, progeny obtained from each cross was confirmed by differences in leaf and flower morphology, plant growth habits, and by acid phosphatase isozyme phenotypes using polyacrylamide gel electrophoresis. In greenhouse inoculation experiments, F1 plants were highly resistant to M. incognita in soil at 25 and 30C. These results confirmed the successful transfer and expression of heat-stable resistance to M. incognita from L. peruvianum to hybrids with L. esculentum as a preliminary step to introgressing additional root-knot nematode resistance into tomato. EEA AMBA Fil: Cap, Guillermo Bartolome. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Área Metropolitana de Buenos Aires. Agencia de Extensión Rural La Plata; Argentina Fil: Roberts, P.A. University of California, Riverside. Department of Hematology; Estados Unidos Fil: Thomason, I.J. University of California, Riverside. Department of Hematology; Estados Unidos Fil: Murashige, T. University of California, Riverside. Department of Hematology; Estados Unidos 2020-05-04T13:36:49Z 2020-05-04T13:36:49Z 1991-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://journals.ashs.org/jashs/view/journals/jashs/116/6/article-p1082.xml http://hdl.handle.net/20.500.12123/7184 0003-1062 2327-9788 https://doi.org/10.21273/JASHS.116.6.1082 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Society for Horticultural Science Journal of the American Society for Horticultural Science 116 (6) : 1082–1088 (November 1991) |
| spellingShingle | Tomate Solanum peruvianum Híbridos Cultivo de Embriones Genotipos Meloidogyne incognita Resistencia a la Enfermedad Tomatoes Hybrids Embryo Culture Genotypes Disease Resistance Lycopersicon esculentum Lycopersicon peruvianum Cap, Guillermo Bartolome Roberts, P.A. Thomason, I.J. Murashige, T. Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita |
| title | Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita |
| title_full | Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita |
| title_fullStr | Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita |
| title_full_unstemmed | Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita |
| title_short | Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita |
| title_sort | embryo culture of lycopersicon esculentum l peruvianum hybrid genotypes possessing heat stable resistance to meloidogyne incognita |
| topic | Tomate Solanum peruvianum Híbridos Cultivo de Embriones Genotipos Meloidogyne incognita Resistencia a la Enfermedad Tomatoes Hybrids Embryo Culture Genotypes Disease Resistance Lycopersicon esculentum Lycopersicon peruvianum |
| url | https://journals.ashs.org/jashs/view/journals/jashs/116/6/article-p1082.xml http://hdl.handle.net/20.500.12123/7184 https://doi.org/10.21273/JASHS.116.6.1082 |
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