Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism
In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
The American Society of Plant Biologists
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7126 http://www.plantcell.org/content/31/9/2070 https://doi.org/10.1105/tpc.18.00929 |
| _version_ | 1855483745322139648 |
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| author | Vanhaelewyn, Lucas Viczián, András Prinsen, Els Bernula, Péter Serrano, Alejandro Miguel Arana, María Veronica Ballaré, Carlos Luis Nagy, Ferenc Van Der Straeten, Dominique Vandenbussche, Filip |
| author_browse | Arana, María Veronica Ballaré, Carlos Luis Bernula, Péter Nagy, Ferenc Prinsen, Els Serrano, Alejandro Miguel Van Der Straeten, Dominique Vandenbussche, Filip Vanhaelewyn, Lucas Viczián, András |
| author_facet | Vanhaelewyn, Lucas Viczián, András Prinsen, Els Bernula, Péter Serrano, Alejandro Miguel Arana, María Veronica Ballaré, Carlos Luis Nagy, Ferenc Van Der Straeten, Dominique Vandenbussche, Filip |
| author_sort | Vanhaelewyn, Lucas |
| collection | INTA Digital |
| description | In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8) serves as the predominant photoreceptor of UVB– induced phototropic responses in Arabidopsis (Arabidopsis thaliana) inflorescence stems. We examined the molecular mechanisms underlying this response and our findings support the Blaauw theory (Blaauw, 1919), suggesting rapid differential growth through unilateral photomorphogenic growth inhibition. UVR8-dependent UV-B light perception occurs mainly in the epidermis and cortex, but deeper tissues such as endodermis can also contribute. Within stems, a spatial difference of UVR8 signal causes a transcript and protein increase of transcription factors ELONGATED HYPOCOTYL5 (HY5) and its homolog HY5 HOMOLOG at the UV-B–exposed side. The irradiated side shows (1) strong activation of flavonoid synthesis genes and flavonoid accumulation; (2) increased gibberellin (GA)2-oxidase expression, diminished GA1 levels, and accumulation of the DELLA protein REPRESSOR OF GA1; and (3) increased expression of the auxin transport regulator
PINOID, contributing to diminished auxin signaling. Together, the data suggest a mechanism of phototropin-independent inflorescence phototropism through multiple, locally UVR8-regulated hormone pathways . |
| format | Artículo |
| id | INTA7126 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | The American Society of Plant Biologists |
| publisherStr | The American Society of Plant Biologists |
| record_format | dspace |
| spelling | INTA71262020-04-22T18:53:37Z Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism Vanhaelewyn, Lucas Viczián, András Prinsen, Els Bernula, Péter Serrano, Alejandro Miguel Arana, María Veronica Ballaré, Carlos Luis Nagy, Ferenc Van Der Straeten, Dominique Vandenbussche, Filip Fisiología Vegetal Fototropismo Arabidopsis Thaliana Fotorreceptores Plant Physiology Phototropism Photoreceptors UVR8 In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8) serves as the predominant photoreceptor of UVB– induced phototropic responses in Arabidopsis (Arabidopsis thaliana) inflorescence stems. We examined the molecular mechanisms underlying this response and our findings support the Blaauw theory (Blaauw, 1919), suggesting rapid differential growth through unilateral photomorphogenic growth inhibition. UVR8-dependent UV-B light perception occurs mainly in the epidermis and cortex, but deeper tissues such as endodermis can also contribute. Within stems, a spatial difference of UVR8 signal causes a transcript and protein increase of transcription factors ELONGATED HYPOCOTYL5 (HY5) and its homolog HY5 HOMOLOG at the UV-B–exposed side. The irradiated side shows (1) strong activation of flavonoid synthesis genes and flavonoid accumulation; (2) increased gibberellin (GA)2-oxidase expression, diminished GA1 levels, and accumulation of the DELLA protein REPRESSOR OF GA1; and (3) increased expression of the auxin transport regulator PINOID, contributing to diminished auxin signaling. Together, the data suggest a mechanism of phototropin-independent inflorescence phototropism through multiple, locally UVR8-regulated hormone pathways . Estación Experimental Agropecuaria Bariloche Fil: Vanhaelewyn, Lucas. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; Bélgica Fil: Viczián, András. Institute of Plant Biology. Biological Research Centre; Hungría Fil: Prinsen, Els. University of Antwerp. Department of Biology; Bélgica Fil: Bernula, Péter. Institute of Plant Biology. Biological Research Centre. University of Szeged. Faculty of Science and Informatics; Hungría Fil: Serrano, Alejandro Miguel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto Argentino de Investigaciones de Zonas Aridas (IADIZA); Argentina Fil: Arana, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Ballaré, Carlos Luis. Universidad Nacional de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiologicas y Ecologicas Vinculadas a la Agricultura (IFEVA); Argentina Fil: Nagy, Ferenc. Institute of Plant Biology. Biological Research Centre; Hungría Fil: Van Der Straeten, Dominique. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; Bélgica Fil: Vandenbussche, Filip. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; Bélgica 2020-04-22T18:37:31Z 2020-04-22T18:37:31Z 2019-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7126 http://www.plantcell.org/content/31/9/2070 1040-4651 1532-298X https://doi.org/10.1105/tpc.18.00929 eng info:eu-repo/semantics/restrictedAccess application/pdf The American Society of Plant Biologists The Plant Cell 31: 2070–2088 (Septiembre 2019) |
| spellingShingle | Fisiología Vegetal Fototropismo Arabidopsis Thaliana Fotorreceptores Plant Physiology Phototropism Photoreceptors UVR8 Vanhaelewyn, Lucas Viczián, András Prinsen, Els Bernula, Péter Serrano, Alejandro Miguel Arana, María Veronica Ballaré, Carlos Luis Nagy, Ferenc Van Der Straeten, Dominique Vandenbussche, Filip Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism |
| title | Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism |
| title_full | Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism |
| title_fullStr | Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism |
| title_full_unstemmed | Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism |
| title_short | Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism |
| title_sort | differential uvr8 signal across the stem controls uv b induced inflorescence phototropism |
| topic | Fisiología Vegetal Fototropismo Arabidopsis Thaliana Fotorreceptores Plant Physiology Phototropism Photoreceptors UVR8 |
| url | http://hdl.handle.net/20.500.12123/7126 http://www.plantcell.org/content/31/9/2070 https://doi.org/10.1105/tpc.18.00929 |
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