Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
Phosphatidylinositol 3-phosphate (PtdIns3P) is a signaling phospholipid, which regulates several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3P mode of action, specifically through effector proteins have been...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7067 https://link.springer.com/article/10.1007%2Fs00425-020-03354-w https://doi.org/10.1007/s00425-020-03354-w |
| _version_ | 1855035790807007232 |
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| author | Agudelo Romero, Patricia Margarida Fortes, Ana Suárez, Trinidad Lascano, Hernan Ramiro Saavedra, Laura |
| author_browse | Agudelo Romero, Patricia Lascano, Hernan Ramiro Margarida Fortes, Ana Saavedra, Laura Suárez, Trinidad |
| author_facet | Agudelo Romero, Patricia Margarida Fortes, Ana Suárez, Trinidad Lascano, Hernan Ramiro Saavedra, Laura |
| author_sort | Agudelo Romero, Patricia |
| collection | INTA Digital |
| description | Phosphatidylinositol 3-phosphate (PtdIns3P) is a signaling phospholipid, which regulates several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3P mode of action, specifically through effector proteins have been partially explored in plants, with main focus on Arabidopsis thaliana. In this study, we searched for genes coding for PtdIns3P-binding proteins such as FYVE and PHOX domain-containing sequences from different photosynthetic organisms to gather evolutionary insights on these phosphoinositide binding domains, followed by an in silico characterization of the FYVE and PHOX gene families in the moss Physcomitrella patens. Phylogenetic analysis showed that PpFYVE proteins can be grouped in 7 subclasses, with an additional subclass whose FYVE domain was lost during evolution to higher plants. On the other hand, PpPHOX proteins are classified into 5 subclasses. Expression analyses based on RNAseq data together with the analysis of cis-acting regulatory elements and transcription factor (TF) binding sites in promoter regions suggest the importance of these proteins in regulating stress responses but mainly developmental processes in P. patens. The results provide valuable information and robust candidate genes for future functional analysis aiming to further explore the role of this signaling pathway mainly during growth and development of tip growing cells and during the transition from 2 to 3D growth. These studies would identify ancestral regulatory players undertaken during plant evolution. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7067 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA70672020-04-13T14:00:29Z Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens Agudelo Romero, Patricia Margarida Fortes, Ana Suárez, Trinidad Lascano, Hernan Ramiro Saavedra, Laura Bryophyta Fosfatidilinositoles Genética Phosphatidylinositols Genetics Physcomitrella patens Musgos Mosses Phosphatidylinositol 3-phosphate (PtdIns3P) is a signaling phospholipid, which regulates several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3P mode of action, specifically through effector proteins have been partially explored in plants, with main focus on Arabidopsis thaliana. In this study, we searched for genes coding for PtdIns3P-binding proteins such as FYVE and PHOX domain-containing sequences from different photosynthetic organisms to gather evolutionary insights on these phosphoinositide binding domains, followed by an in silico characterization of the FYVE and PHOX gene families in the moss Physcomitrella patens. Phylogenetic analysis showed that PpFYVE proteins can be grouped in 7 subclasses, with an additional subclass whose FYVE domain was lost during evolution to higher plants. On the other hand, PpPHOX proteins are classified into 5 subclasses. Expression analyses based on RNAseq data together with the analysis of cis-acting regulatory elements and transcription factor (TF) binding sites in promoter regions suggest the importance of these proteins in regulating stress responses but mainly developmental processes in P. patens. The results provide valuable information and robust candidate genes for future functional analysis aiming to further explore the role of this signaling pathway mainly during growth and development of tip growing cells and during the transition from 2 to 3D growth. These studies would identify ancestral regulatory players undertaken during plant evolution. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Agudelo Romero, Patricia. University of Western Australia. Institute of Agriculture; Australia. University of Western Australia. ARC Centre of Excellence in Plant Energy Biology; Australia Fil: Margarida Fortes, Ana. Universidade de Lisboa. Faculdade de Ciências. BioISI-Biosystems and Integrative Sciences Institute; Portugal Fil: Suárez, Trinidad. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Saavedra, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas (IIByT). Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina 2020-04-13T13:53:12Z 2020-04-13T13:53:12Z 2020-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7067 https://link.springer.com/article/10.1007%2Fs00425-020-03354-w 0032-0935 1432-2048 https://doi.org/10.1007/s00425-020-03354-w eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Planta 251 : Article number 62 (2020) |
| spellingShingle | Bryophyta Fosfatidilinositoles Genética Phosphatidylinositols Genetics Physcomitrella patens Musgos Mosses Agudelo Romero, Patricia Margarida Fortes, Ana Suárez, Trinidad Lascano, Hernan Ramiro Saavedra, Laura Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens |
| title | Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens |
| title_full | Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens |
| title_fullStr | Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens |
| title_full_unstemmed | Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens |
| title_short | Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens |
| title_sort | evolutionary insights into fyve and phox effector proteins from the moss physcomitrella patens |
| topic | Bryophyta Fosfatidilinositoles Genética Phosphatidylinositols Genetics Physcomitrella patens Musgos Mosses |
| url | http://hdl.handle.net/20.500.12123/7067 https://link.springer.com/article/10.1007%2Fs00425-020-03354-w https://doi.org/10.1007/s00425-020-03354-w |
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