Molecular variability of watermelon mosaic virus isolates from Argentina
Watermelon mosaic virus (WMV) is an economically important virus of cucurbit crops in Argentina. The available information on genetic variability must be continuously updated. In this study, we assessed the molecular variability of WMV isolates in Argentina based on the partial sequences of the NIb-...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7065 https://link.springer.com/article/10.1007/s10658-020-01965-3 https://doi.org/10.1007/s10658-020-01965-3 |
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| author | Pozzi, Elizabeth Alicia Perotto, Maria Cecilia Bertin, S. Manglli, A. Luciani, Cecilia Elizabeth Conci, Vilma Cecilia Tomassoli, L. |
| author_browse | Bertin, S. Conci, Vilma Cecilia Luciani, Cecilia Elizabeth Manglli, A. Perotto, Maria Cecilia Pozzi, Elizabeth Alicia Tomassoli, L. |
| author_facet | Pozzi, Elizabeth Alicia Perotto, Maria Cecilia Bertin, S. Manglli, A. Luciani, Cecilia Elizabeth Conci, Vilma Cecilia Tomassoli, L. |
| author_sort | Pozzi, Elizabeth Alicia |
| collection | INTA Digital |
| description | Watermelon mosaic virus (WMV) is an economically important virus of cucurbit crops in Argentina. The available information on genetic variability must be continuously updated. In this study, we assessed the molecular variability of WMV isolates in Argentina based on the partial sequences of the NIb-CP region and compared them with isolates previously reported from around the world. Forty-six WMV isolates were obtained from naturally infected cucurbit crops collected from 10 provinces between 2011 and 2018. At the molecular level, WMV isolates were grouped into three distinct major phylogenetic groups based on genetic distance. Majority of the Argentine isolates belonged to the emerging group (G3), whereas one isolate was included in G1 and another cluster in G2. G3 was further divided into 5 subgroups, named EM1, EM2, EM3, EM4, and a new group “EMArg”, composed exclusively of Argentine isolates with high bootstrap support and high level of significance. Tajima’s D and Fu were significantly negative for G3 and EMArg, indicating that the population of this subgroup has recently expanded. AMOVA analysis showed that the isolates was not well correlated with their geographic origin. The fixation index (FST) value between the WMV isolates from the different provinces in Argentina was lower than 0.33, indicating a relatively frequent gene flow between provinces. In every group, the predominant evolutionary pressure was negative with a mean dN/dS < 1, suggesting a slow replacement fixation rate. One recombination event was detected involving isolates of EM2 cluster from Argentina. |
| format | Artículo |
| id | INTA7065 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA70652022-11-09T11:27:29Z Molecular variability of watermelon mosaic virus isolates from Argentina Pozzi, Elizabeth Alicia Perotto, Maria Cecilia Bertin, S. Manglli, A. Luciani, Cecilia Elizabeth Conci, Vilma Cecilia Tomassoli, L. Sandia Enfermedades de las Plantas Variación Genética Argentina Watermelons Plant Diseases Watermelon Mosaic Virus Genetic Variation Virus del Mosaico de la Sandia Watermelon mosaic virus (WMV) is an economically important virus of cucurbit crops in Argentina. The available information on genetic variability must be continuously updated. In this study, we assessed the molecular variability of WMV isolates in Argentina based on the partial sequences of the NIb-CP region and compared them with isolates previously reported from around the world. Forty-six WMV isolates were obtained from naturally infected cucurbit crops collected from 10 provinces between 2011 and 2018. At the molecular level, WMV isolates were grouped into three distinct major phylogenetic groups based on genetic distance. Majority of the Argentine isolates belonged to the emerging group (G3), whereas one isolate was included in G1 and another cluster in G2. G3 was further divided into 5 subgroups, named EM1, EM2, EM3, EM4, and a new group “EMArg”, composed exclusively of Argentine isolates with high bootstrap support and high level of significance. Tajima’s D and Fu were significantly negative for G3 and EMArg, indicating that the population of this subgroup has recently expanded. AMOVA analysis showed that the isolates was not well correlated with their geographic origin. The fixation index (FST) value between the WMV isolates from the different provinces in Argentina was lower than 0.33, indicating a relatively frequent gene flow between provinces. In every group, the predominant evolutionary pressure was negative with a mean dN/dS < 1, suggesting a slow replacement fixation rate. One recombination event was detected involving isolates of EM2 cluster from Argentina. Instituto de Patología Vegetal Fil: Pozzi, E.A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bertin, S. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; Italia Fil: Manglli, A. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; Italia Fil: Luciani, Cecilia E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tomassoli, L. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; Italia 2020-04-09T14:21:32Z 2020-04-09T14:21:32Z 2020-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7065 https://link.springer.com/article/10.1007/s10658-020-01965-3 0929-1873 1573-8469 https://doi.org/10.1007/s10658-020-01965-3 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer European Journal of Plant Pathology (2020) |
| spellingShingle | Sandia Enfermedades de las Plantas Variación Genética Argentina Watermelons Plant Diseases Watermelon Mosaic Virus Genetic Variation Virus del Mosaico de la Sandia Pozzi, Elizabeth Alicia Perotto, Maria Cecilia Bertin, S. Manglli, A. Luciani, Cecilia Elizabeth Conci, Vilma Cecilia Tomassoli, L. Molecular variability of watermelon mosaic virus isolates from Argentina |
| title | Molecular variability of watermelon mosaic virus isolates from Argentina |
| title_full | Molecular variability of watermelon mosaic virus isolates from Argentina |
| title_fullStr | Molecular variability of watermelon mosaic virus isolates from Argentina |
| title_full_unstemmed | Molecular variability of watermelon mosaic virus isolates from Argentina |
| title_short | Molecular variability of watermelon mosaic virus isolates from Argentina |
| title_sort | molecular variability of watermelon mosaic virus isolates from argentina |
| topic | Sandia Enfermedades de las Plantas Variación Genética Argentina Watermelons Plant Diseases Watermelon Mosaic Virus Genetic Variation Virus del Mosaico de la Sandia |
| url | http://hdl.handle.net/20.500.12123/7065 https://link.springer.com/article/10.1007/s10658-020-01965-3 https://doi.org/10.1007/s10658-020-01965-3 |
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