Clinal variation along precipitation gradients in Patagonian temperate forests: unravelling demographic and selection signatures in three Nothofagus spp.
Past demographic changes and current selection pressures determine the genetic variation displayed by Nothofagus species along rainfall gradients. Based on the diversity trends observed at candidate genes associated to drought stress, we inferred a differential species’ adaptive potential. Context...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer Nature
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7039 https://link.springer.com/article/10.1007/s13595-019-0908-x https://doi.org/10.1007/s13595-019-0908-x |
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| author | Soliani, Carolina Azpilicueta, Maria Marta Arana, María Veronica Marchelli, Paula |
| author_browse | Arana, María Veronica Azpilicueta, Maria Marta Marchelli, Paula Soliani, Carolina |
| author_facet | Soliani, Carolina Azpilicueta, Maria Marta Arana, María Veronica Marchelli, Paula |
| author_sort | Soliani, Carolina |
| collection | INTA Digital |
| description | Past demographic changes and current selection pressures determine the genetic variation displayed by Nothofagus species along rainfall gradients. Based on the diversity trends observed at candidate genes associated to drought stress, we inferred a differential species’ adaptive potential.
Context Clinal genetic variation in natural populations could reflect either recent demographic history or the evolution of adapted genotypes along heterogeneous environments.
Aims We describe genetic variation patterns in three Nothofagus species of South American temperate forests, growing along steep rainfall gradients. Our hypothesis is that the selection pressure along this gradient reinforces the genetic structure previously shaped by Pleistocene climate oscillations.
Methods We screened variation along gradients at putative adaptive markers: candidate genes involved in response to drought, and EST-SSRs linked to drought stress genes. Genomic SSRs (gSSRs) were used to decouple the incidence of demographic events in the genetic structure.
Results Genetic diversity at SSRs agreed with the putative location of cryptic Pleistocene refugia in Nothofagus. In addition, each species showed different trends for nucleotide diversity at candidate genes. Unbiased heterozygosity significantly correlated with precipitation at EST-SSRs in Nothofagus nervosa. We found evidences of balancing selection and several SNPs departed from neutral expectations.
Conclusions Nothofagus genetic variability shows a strong imprint of demographic changes that reveals refugia location for the
species during Pleistocene. This variability is modelled by environmental conditions across natural gradients, which impose selection pressure at genome regions related to stress response, providing clues about inter-specific differences in adaptive potential to water deficit. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7039 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INTA70392020-04-06T12:18:29Z Clinal variation along precipitation gradients in Patagonian temperate forests: unravelling demographic and selection signatures in three Nothofagus spp. Soliani, Carolina Azpilicueta, Maria Marta Arana, María Veronica Marchelli, Paula Nothofagus Variación Genética Estrés de Sequía Resistencia a la Sequía Bosques Genetic Variation Drought Stress Drought Resistance Forests Genética Forestal Gradientes Naturales Past demographic changes and current selection pressures determine the genetic variation displayed by Nothofagus species along rainfall gradients. Based on the diversity trends observed at candidate genes associated to drought stress, we inferred a differential species’ adaptive potential. Context Clinal genetic variation in natural populations could reflect either recent demographic history or the evolution of adapted genotypes along heterogeneous environments. Aims We describe genetic variation patterns in three Nothofagus species of South American temperate forests, growing along steep rainfall gradients. Our hypothesis is that the selection pressure along this gradient reinforces the genetic structure previously shaped by Pleistocene climate oscillations. Methods We screened variation along gradients at putative adaptive markers: candidate genes involved in response to drought, and EST-SSRs linked to drought stress genes. Genomic SSRs (gSSRs) were used to decouple the incidence of demographic events in the genetic structure. Results Genetic diversity at SSRs agreed with the putative location of cryptic Pleistocene refugia in Nothofagus. In addition, each species showed different trends for nucleotide diversity at candidate genes. Unbiased heterozygosity significantly correlated with precipitation at EST-SSRs in Nothofagus nervosa. We found evidences of balancing selection and several SNPs departed from neutral expectations. Conclusions Nothofagus genetic variability shows a strong imprint of demographic changes that reveals refugia location for the species during Pleistocene. This variability is modelled by environmental conditions across natural gradients, which impose selection pressure at genome regions related to stress response, providing clues about inter-specific differences in adaptive potential to water deficit. Fil: Soliani, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Azpilicueta, Maria Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Arana, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina 2020-04-06T12:09:40Z 2020-04-06T12:09:40Z 2020-01-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7039 https://link.springer.com/article/10.1007/s13595-019-0908-x 1286-4560 1297-966X https://doi.org/10.1007/s13595-019-0908-x eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Nature Annals of Forest Science 77 : 4 (January 2020) |
| spellingShingle | Nothofagus Variación Genética Estrés de Sequía Resistencia a la Sequía Bosques Genetic Variation Drought Stress Drought Resistance Forests Genética Forestal Gradientes Naturales Soliani, Carolina Azpilicueta, Maria Marta Arana, María Veronica Marchelli, Paula Clinal variation along precipitation gradients in Patagonian temperate forests: unravelling demographic and selection signatures in three Nothofagus spp. |
| title | Clinal variation along precipitation gradients in Patagonian temperate forests: unravelling demographic and selection signatures in three Nothofagus spp. |
| title_full | Clinal variation along precipitation gradients in Patagonian temperate forests: unravelling demographic and selection signatures in three Nothofagus spp. |
| title_fullStr | Clinal variation along precipitation gradients in Patagonian temperate forests: unravelling demographic and selection signatures in three Nothofagus spp. |
| title_full_unstemmed | Clinal variation along precipitation gradients in Patagonian temperate forests: unravelling demographic and selection signatures in three Nothofagus spp. |
| title_short | Clinal variation along precipitation gradients in Patagonian temperate forests: unravelling demographic and selection signatures in three Nothofagus spp. |
| title_sort | clinal variation along precipitation gradients in patagonian temperate forests unravelling demographic and selection signatures in three nothofagus spp |
| topic | Nothofagus Variación Genética Estrés de Sequía Resistencia a la Sequía Bosques Genetic Variation Drought Stress Drought Resistance Forests Genética Forestal Gradientes Naturales |
| url | http://hdl.handle.net/20.500.12123/7039 https://link.springer.com/article/10.1007/s13595-019-0908-x https://doi.org/10.1007/s13595-019-0908-x |
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