Risk factors of S. aureus intramammary infection in pre partum dairy heifers under grazing conditions and molecular characterization of isolates from heifers and cows
The aims of the research reported here were to identify potential risk factors associated with the presence of Staphylococcus aureus intramammary infection (IMI) in pre partum dairy heifers on 17 dairy farms from three provinces of Argentina and to characterize, at molecular level, isolates from tho...
| Autores principales: | , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Cambridge University Press
2020
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7022 https://doi.org/10.1017/S0022029919001018 |
| _version_ | 1855035779940614144 |
|---|---|
| author | Camussone, Cecilia Molineri, Ana Ines Signorini, Marcelo Neder, Veronica Elizabeth Vitulich, Carlos Alberto Calvinho, Luis Fernando |
| author_browse | Calvinho, Luis Fernando Camussone, Cecilia Molineri, Ana Ines Neder, Veronica Elizabeth Signorini, Marcelo Vitulich, Carlos Alberto |
| author_facet | Camussone, Cecilia Molineri, Ana Ines Signorini, Marcelo Neder, Veronica Elizabeth Vitulich, Carlos Alberto Calvinho, Luis Fernando |
| author_sort | Camussone, Cecilia |
| collection | INTA Digital |
| description | The aims of the research reported here were to identify potential risk factors associated with the presence of Staphylococcus aureus intramammary infection (IMI) in pre partum dairy heifers on 17 dairy farms from three provinces of Argentina and to characterize, at molecular level, isolates from those heifers and lactating cows from two selected herds. A total of 1474 heifers and 4878 lactating cows were studied. The prevalence of Staphylococcus aureus IMI in the heifers, heifers at quarter level and lactating cow mammary quarters was 14.41, 4.82, and 14.65%, respectively. Univariate analysis showed the key variables associated with S. aureus IMI presence in the heifers were: S. aureus IMI prevalence in cows of the lactating herd, the time calves stayed with their dam after birth, the calf rearing system, the place of rearing (own farm or other dairy farm) and fly control on the farm. None of the variables included in the multivariable analysis was associated with the presence of S. aureus IMI in the pre partum heifers, probably due to low variability among management practices used by the farms for rearing the heifer calves. At the molecular level, S. aureus isolates were grouped into three main PFGE clusters and several genotypes within the clusters. Isolates from mammary secretion of pre partum heifers and milk of lactating cows comprised different PFGE clusters in both herds, although two exceptions occurred. The absence of gene fnbpB, which codifies for a virulence factor protein involved in cell invasion by S. aureus, was significantly more frequent in pre partum heifer secretion isolates than in isolates from lactating cow milk. These results suggest that, under these management conditions, isolates from mammary secretions of pre partum heifers do not originate from the milk of lactating cows, but rather other sources to which the heifer is exposed. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7022 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | INTA70222020-04-02T12:39:03Z Risk factors of S. aureus intramammary infection in pre partum dairy heifers under grazing conditions and molecular characterization of isolates from heifers and cows Camussone, Cecilia Molineri, Ana Ines Signorini, Marcelo Neder, Veronica Elizabeth Vitulich, Carlos Alberto Calvinho, Luis Fernando Vacas Lecheras Novilla Factores de Riesgo Biología Molecular Dairy Cows Heifers Risk Factors Staphylococcus Aureus Molecular Biology Intramammary Infection Molecular Characterization Infección Intramamaria Caracterización Molecular The aims of the research reported here were to identify potential risk factors associated with the presence of Staphylococcus aureus intramammary infection (IMI) in pre partum dairy heifers on 17 dairy farms from three provinces of Argentina and to characterize, at molecular level, isolates from those heifers and lactating cows from two selected herds. A total of 1474 heifers and 4878 lactating cows were studied. The prevalence of Staphylococcus aureus IMI in the heifers, heifers at quarter level and lactating cow mammary quarters was 14.41, 4.82, and 14.65%, respectively. Univariate analysis showed the key variables associated with S. aureus IMI presence in the heifers were: S. aureus IMI prevalence in cows of the lactating herd, the time calves stayed with their dam after birth, the calf rearing system, the place of rearing (own farm or other dairy farm) and fly control on the farm. None of the variables included in the multivariable analysis was associated with the presence of S. aureus IMI in the pre partum heifers, probably due to low variability among management practices used by the farms for rearing the heifer calves. At the molecular level, S. aureus isolates were grouped into three main PFGE clusters and several genotypes within the clusters. Isolates from mammary secretion of pre partum heifers and milk of lactating cows comprised different PFGE clusters in both herds, although two exceptions occurred. The absence of gene fnbpB, which codifies for a virulence factor protein involved in cell invasion by S. aureus, was significantly more frequent in pre partum heifer secretion isolates than in isolates from lactating cow milk. These results suggest that, under these management conditions, isolates from mammary secretions of pre partum heifers do not originate from the milk of lactating cows, but rather other sources to which the heifer is exposed. EEA Rafaela Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Molineri, Ana Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Neder, Veronica Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Fil: Vitulich, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. 2020-04-02T12:29:44Z 2020-04-02T12:29:44Z 2020-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7022 0022-0299 1469-7629 (Online) https://doi.org/10.1017/S0022029919001018 eng info:eu-repo/semantics/restrictedAccess application/pdf Cambridge University Press Journal of dairy research 87 (1) : 82-88. (February 2020) |
| spellingShingle | Vacas Lecheras Novilla Factores de Riesgo Biología Molecular Dairy Cows Heifers Risk Factors Staphylococcus Aureus Molecular Biology Intramammary Infection Molecular Characterization Infección Intramamaria Caracterización Molecular Camussone, Cecilia Molineri, Ana Ines Signorini, Marcelo Neder, Veronica Elizabeth Vitulich, Carlos Alberto Calvinho, Luis Fernando Risk factors of S. aureus intramammary infection in pre partum dairy heifers under grazing conditions and molecular characterization of isolates from heifers and cows |
| title | Risk factors of S. aureus intramammary infection in pre partum dairy heifers under grazing conditions and molecular characterization of isolates from heifers and cows |
| title_full | Risk factors of S. aureus intramammary infection in pre partum dairy heifers under grazing conditions and molecular characterization of isolates from heifers and cows |
| title_fullStr | Risk factors of S. aureus intramammary infection in pre partum dairy heifers under grazing conditions and molecular characterization of isolates from heifers and cows |
| title_full_unstemmed | Risk factors of S. aureus intramammary infection in pre partum dairy heifers under grazing conditions and molecular characterization of isolates from heifers and cows |
| title_short | Risk factors of S. aureus intramammary infection in pre partum dairy heifers under grazing conditions and molecular characterization of isolates from heifers and cows |
| title_sort | risk factors of s aureus intramammary infection in pre partum dairy heifers under grazing conditions and molecular characterization of isolates from heifers and cows |
| topic | Vacas Lecheras Novilla Factores de Riesgo Biología Molecular Dairy Cows Heifers Risk Factors Staphylococcus Aureus Molecular Biology Intramammary Infection Molecular Characterization Infección Intramamaria Caracterización Molecular |
| url | http://hdl.handle.net/20.500.12123/7022 https://doi.org/10.1017/S0022029919001018 |
| work_keys_str_mv | AT camussonececilia riskfactorsofsaureusintramammaryinfectioninprepartumdairyheifersundergrazingconditionsandmolecularcharacterizationofisolatesfromheifersandcows AT molinerianaines riskfactorsofsaureusintramammaryinfectioninprepartumdairyheifersundergrazingconditionsandmolecularcharacterizationofisolatesfromheifersandcows AT signorinimarcelo riskfactorsofsaureusintramammaryinfectioninprepartumdairyheifersundergrazingconditionsandmolecularcharacterizationofisolatesfromheifersandcows AT nederveronicaelizabeth riskfactorsofsaureusintramammaryinfectioninprepartumdairyheifersundergrazingconditionsandmolecularcharacterizationofisolatesfromheifersandcows AT vitulichcarlosalberto riskfactorsofsaureusintramammaryinfectioninprepartumdairyheifersundergrazingconditionsandmolecularcharacterizationofisolatesfromheifersandcows AT calvinholuisfernando riskfactorsofsaureusintramammaryinfectioninprepartumdairyheifersundergrazingconditionsandmolecularcharacterizationofisolatesfromheifersandcows |