Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics
In northwestern Argentina (NWA), pepper crops are threatened by the emergence of begomoviruses due to the spread of its vector, Bemisia tabaci (Gennadius). The genus Begomovirus includes pathogens that can have a monopartite or bipartite genome and are occasionally associated with sub-viral particle...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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MDPI
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7002 https://www.mdpi.com/1999-4915/12/2/202 https://doi.org/10.3390/v12020202 |
| _version_ | 1855483921972592640 |
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| author | Bornancini, Veronica Andrea Irazoqui, Jose Matias Flores, Ceferino Rene Vaghi Medina, Carlos Gaston Amadio, Ariel Lopez Lambertini, Paola Maria |
| author_browse | Amadio, Ariel Bornancini, Veronica Andrea Flores, Ceferino Rene Irazoqui, Jose Matias Lopez Lambertini, Paola Maria Vaghi Medina, Carlos Gaston |
| author_facet | Bornancini, Veronica Andrea Irazoqui, Jose Matias Flores, Ceferino Rene Vaghi Medina, Carlos Gaston Amadio, Ariel Lopez Lambertini, Paola Maria |
| author_sort | Bornancini, Veronica Andrea |
| collection | INTA Digital |
| description | In northwestern Argentina (NWA), pepper crops are threatened by the emergence of begomoviruses due to the spread of its vector, Bemisia tabaci (Gennadius). The genus Begomovirus includes pathogens that can have a monopartite or bipartite genome and are occasionally associated with sub-viral particles called satellites. This study characterized the diversity of begomovirus and alphasatellite species infecting pepper in NWA using a metagenomic approach. Using RCA-NGS (rolling circle amplification-next generation sequencing), 19 full-length begomovirus genomes (DNA-A and DNA-B) and one alphasatellite were assembled. This ecogenomic approach revealed six begomoviruses in single infections: soybean blistering mosaic virus (SbBMV), tomato yellow spot virus (ToYSV), tomato yellow vein streak virus (ToYVSV), tomato dwarf leaf virus (ToDfLV), sida golden mosaic Brazil virus (SiGMBRV), and a new proposed species, named pepper blistering leaf virus (PepBLV). SbBMV was the most frequently detected species, followed by ToYSV. Moreover, a new alphasatellite associated with ToYSV, named tomato yellow spot alphasatellite 2 (ToYSA-2), was reported for the first time in Argentina. For the Americas, this was the first report of an alphasatellite found in a crop (pepper) and in a weed (Leonurus japonicus). We also detected intra-species and inter-species recombination |
| format | Artículo |
| id | INTA7002 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA70022020-03-27T20:26:31Z Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics Bornancini, Veronica Andrea Irazoqui, Jose Matias Flores, Ceferino Rene Vaghi Medina, Carlos Gaston Amadio, Ariel Lopez Lambertini, Paola Maria Begomovirus Geminiviridae Geminivirus Pimienta Virus Satélite Recombinación Pepper Satellite Viruses Recombination Geminialphasatellitinae In northwestern Argentina (NWA), pepper crops are threatened by the emergence of begomoviruses due to the spread of its vector, Bemisia tabaci (Gennadius). The genus Begomovirus includes pathogens that can have a monopartite or bipartite genome and are occasionally associated with sub-viral particles called satellites. This study characterized the diversity of begomovirus and alphasatellite species infecting pepper in NWA using a metagenomic approach. Using RCA-NGS (rolling circle amplification-next generation sequencing), 19 full-length begomovirus genomes (DNA-A and DNA-B) and one alphasatellite were assembled. This ecogenomic approach revealed six begomoviruses in single infections: soybean blistering mosaic virus (SbBMV), tomato yellow spot virus (ToYSV), tomato yellow vein streak virus (ToYVSV), tomato dwarf leaf virus (ToDfLV), sida golden mosaic Brazil virus (SiGMBRV), and a new proposed species, named pepper blistering leaf virus (PepBLV). SbBMV was the most frequently detected species, followed by ToYSV. Moreover, a new alphasatellite associated with ToYSV, named tomato yellow spot alphasatellite 2 (ToYSA-2), was reported for the first time in Argentina. For the Americas, this was the first report of an alphasatellite found in a crop (pepper) and in a weed (Leonurus japonicus). We also detected intra-species and inter-species recombination Instituto de Virología Fil: Bornancini, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Flores, Ceferino Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina Fil: Vaghi Medina, Carlos Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Lopez Lambertini, Paola Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2020-03-27T18:29:05Z 2020-03-27T18:29:05Z 2020-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7002 https://www.mdpi.com/1999-4915/12/2/202 1999-4915 https://doi.org/10.3390/v12020202 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Viruses 12 (2) : 202. (February 2020) |
| spellingShingle | Begomovirus Geminiviridae Geminivirus Pimienta Virus Satélite Recombinación Pepper Satellite Viruses Recombination Geminialphasatellitinae Bornancini, Veronica Andrea Irazoqui, Jose Matias Flores, Ceferino Rene Vaghi Medina, Carlos Gaston Amadio, Ariel Lopez Lambertini, Paola Maria Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics |
| title | Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics |
| title_full | Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics |
| title_fullStr | Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics |
| title_full_unstemmed | Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics |
| title_short | Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics |
| title_sort | reconstruction and characterization of full length begomovirus and alphasatellite genomes infecting pepper through metagenomics |
| topic | Begomovirus Geminiviridae Geminivirus Pimienta Virus Satélite Recombinación Pepper Satellite Viruses Recombination Geminialphasatellitinae |
| url | http://hdl.handle.net/20.500.12123/7002 https://www.mdpi.com/1999-4915/12/2/202 https://doi.org/10.3390/v12020202 |
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