Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Springer Nature
2020
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/6945 https://www.nature.com/articles/s41598-020-60850-5 https://doi.org/10.1038/s41598-020-60850-5 |
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| author | Romero Victorica, Matias Soria, Marcelo Abel Batista-García, Ramón Alberto Ceja-Navarro, Javier A. Vikram, Surendra Ortiz, Maximiliano Ontañon, Ornella Ghio, Silvina Martínez-Ávila, Liliana Quintero García, Omar Jasiel Etcheverry, Clara Campos, Eleonora Cowan, Donald Arneodo Larochette, Joel Demian Talia, Paola Mónica |
| author_browse | Arneodo Larochette, Joel Demian Batista-García, Ramón Alberto Campos, Eleonora Ceja-Navarro, Javier A. Cowan, Donald Etcheverry, Clara Ghio, Silvina Martínez-Ávila, Liliana Ontañon, Ornella Ortiz, Maximiliano Quintero García, Omar Jasiel Romero Victorica, Matias Soria, Marcelo Abel Talia, Paola Mónica Vikram, Surendra |
| author_facet | Romero Victorica, Matias Soria, Marcelo Abel Batista-García, Ramón Alberto Ceja-Navarro, Javier A. Vikram, Surendra Ortiz, Maximiliano Ontañon, Ornella Ghio, Silvina Martínez-Ávila, Liliana Quintero García, Omar Jasiel Etcheverry, Clara Campos, Eleonora Cowan, Donald Arneodo Larochette, Joel Demian Talia, Paola Mónica |
| author_sort | Romero Victorica, Matias |
| collection | INTA Digital |
| description | In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic
potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite
species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal
was to assess the microbial community compositions and metabolic capacity, and to identify genes
involved in lignocellulose degradation. Individuals from both termite species contained the same five
dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes)
although with different relative abundances. However, detected functional capacity varied, with C.
fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid
metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes
involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome
was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes.
These findings suggest an association between the primary food source and the predicted categories
of the enzymes present in the gut microbiomes of each species. To further investigate the termite
microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-
xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant
metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with
the optimum activity at 50 °C and a pH-activity range from 5 to 10. These characteristics suggest
that Xy110E may be a promising candidate for further development in lignocellulose deconstruction
applications. |
| format | Artículo |
| id | INTA6945 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INTA69452020-03-16T11:32:16Z Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes Romero Victorica, Matias Soria, Marcelo Abel Batista-García, Ramón Alberto Ceja-Navarro, Javier A. Vikram, Surendra Ortiz, Maximiliano Ontañon, Ornella Ghio, Silvina Martínez-Ávila, Liliana Quintero García, Omar Jasiel Etcheverry, Clara Campos, Eleonora Cowan, Donald Arneodo Larochette, Joel Demian Talia, Paola Mónica Genomics Nucleotide Sequence Genómica Secuencia Nucleotídica In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes. These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4- xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications. Instituto de Microbiología y Zoología Agrícola (IMyZA) Fil: Victorica, Matias Romero. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Batista-García, Ramón Alberto. Universidad Autónoma Estado Morelos; México Fil: Ceja-Navarro, Javier A. Lawrence Berkeley National Laboratory. Biological Systems and Engineering Division ; Estados Unidos Fil: Vikram, Surendra. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica Fil: Ortiz, Maximiliano. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica Fil: Ontañon, Ornella. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martínez-Ávila, Liliana. Universidad Autónoma Estado Morelos; México Fil: Quintero García, Omar Jasiel. Universidad Autónoma Estado Morelos; México Fil: Etcheverry, Clara. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Biología de los Invertebrados; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cowan, Donald. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-03-16T11:04:38Z 2020-03-16T11:04:38Z 2020-03-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6945 https://www.nature.com/articles/s41598-020-60850-5 2045-2322 https://doi.org/10.1038/s41598-020-60850-5 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Nature Scientific Reports 10 : 3864 (2020) |
| spellingShingle | Genomics Nucleotide Sequence Genómica Secuencia Nucleotídica Romero Victorica, Matias Soria, Marcelo Abel Batista-García, Ramón Alberto Ceja-Navarro, Javier A. Vikram, Surendra Ortiz, Maximiliano Ontañon, Ornella Ghio, Silvina Martínez-Ávila, Liliana Quintero García, Omar Jasiel Etcheverry, Clara Campos, Eleonora Cowan, Donald Arneodo Larochette, Joel Demian Talia, Paola Mónica Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes |
| title | Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes |
| title_full | Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes |
| title_fullStr | Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes |
| title_full_unstemmed | Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes |
| title_short | Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes |
| title_sort | neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes |
| topic | Genomics Nucleotide Sequence Genómica Secuencia Nucleotídica |
| url | http://hdl.handle.net/20.500.12123/6945 https://www.nature.com/articles/s41598-020-60850-5 https://doi.org/10.1038/s41598-020-60850-5 |
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