Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Asian-Australasian Association of Animal Production Societies (AAAP)
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/6913 https://www.ajas.info/current/index.php?vol=12&no=8&special= https://doi.org/10.5713/ajas.19.0163 |
| _version_ | 1855035760761110528 |
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| author | Coria, María Sumampa Reineri, Pablo Sebastian Pighin, Darío Gabriel Barrionuevo, María Guadalupe Carranza, Pedro Gabriel Grigioni, Gabriela Maria Palma, Gustavo Adolfo |
| author_browse | Barrionuevo, María Guadalupe Carranza, Pedro Gabriel Coria, María Sumampa Grigioni, Gabriela Maria Palma, Gustavo Adolfo Pighin, Darío Gabriel Reineri, Pablo Sebastian |
| author_facet | Coria, María Sumampa Reineri, Pablo Sebastian Pighin, Darío Gabriel Barrionuevo, María Guadalupe Carranza, Pedro Gabriel Grigioni, Gabriela Maria Palma, Gustavo Adolfo |
| author_sort | Coria, María Sumampa |
| collection | INTA Digital |
| description | Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization.
Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography.
Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01).
Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6913 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Asian-Australasian Association of Animal Production Societies (AAAP) |
| publisherStr | Asian-Australasian Association of Animal Production Societies (AAAP) |
| record_format | dspace |
| spelling | INTA69132023-04-14T10:14:14Z Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers Coria, María Sumampa Reineri, Pablo Sebastian Pighin, Darío Gabriel Barrionuevo, María Guadalupe Carranza, Pedro Gabriel Grigioni, Gabriela Maria Palma, Gustavo Adolfo Beef Cattle Cattle Feeding Calpain Meat Quality Tenderness Ganado de Carne Alimentación de Ganado Vacuno Calpaina Calidad de la Carne Ternura Calpain System Meat Tenderness Sistema de Calpaína Terneza de la Carne Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins. Fil: Coria, María Sumampa. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina. Fil: Reineri, Pablo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero. Campo Experimental Francisco Cantos; Argentina. Fil: Pighin, Darío Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Universidad de Morón; Argentina. Fil: Barrionuevo, María Guadalupe. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Carranza, Pedro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Transferencia Santiago del Estero; Argentina. Fil: Grigioni, Gabriela Maria. Consejo Nacional de Investigaciones Cientiíficas y Ténicas; Argentina.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Universidad de Morón; Argentina. Fil: Palma, Gustavo Adolfo. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina. 2020-03-10T14:33:10Z 2020-03-10T14:33:10Z 2019-08-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6913 https://www.ajas.info/current/index.php?vol=12&no=8&special= 1011-2367 1976-5517 (Online) https://doi.org/10.5713/ajas.19.0163 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Asian-Australasian Association of Animal Production Societies (AAAP) Asian-Australasian journal animal sciences 12 (8) :1171-1176. (2019) |
| spellingShingle | Beef Cattle Cattle Feeding Calpain Meat Quality Tenderness Ganado de Carne Alimentación de Ganado Vacuno Calpaina Calidad de la Carne Ternura Calpain System Meat Tenderness Sistema de Calpaína Terneza de la Carne Coria, María Sumampa Reineri, Pablo Sebastian Pighin, Darío Gabriel Barrionuevo, María Guadalupe Carranza, Pedro Gabriel Grigioni, Gabriela Maria Palma, Gustavo Adolfo Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers |
| title | Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers |
| title_full | Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers |
| title_fullStr | Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers |
| title_full_unstemmed | Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers |
| title_short | Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers |
| title_sort | feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of braford steers |
| topic | Beef Cattle Cattle Feeding Calpain Meat Quality Tenderness Ganado de Carne Alimentación de Ganado Vacuno Calpaina Calidad de la Carne Ternura Calpain System Meat Tenderness Sistema de Calpaína Terneza de la Carne |
| url | http://hdl.handle.net/20.500.12123/6913 https://www.ajas.info/current/index.php?vol=12&no=8&special= https://doi.org/10.5713/ajas.19.0163 |
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