Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion
The lack of naturally occurring resistance to citrus psorosis virus (CPsV) necessitates a transgenic approach for the development of CPsV-resistant citrus. To evaluate the feasibility of conferring resistance to a non-transgenic scion, we have assembled citrus plants by grafting combining a non-tran...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/6776 http://link.springer.com/10.1007/s11248-020-00191-1 https://doi.org/10.1007/s11248-020-00191-1 |
| _version_ | 1855035735830167552 |
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| author | de Francesco, Agustina Simeone, Melina Gomez, Claudio Andres Costa, Norma Beatriz Garcia, Maria Laura |
| author_browse | Costa, Norma Beatriz Garcia, Maria Laura Gomez, Claudio Andres Simeone, Melina de Francesco, Agustina |
| author_facet | de Francesco, Agustina Simeone, Melina Gomez, Claudio Andres Costa, Norma Beatriz Garcia, Maria Laura |
| author_sort | de Francesco, Agustina |
| collection | INTA Digital |
| description | The lack of naturally occurring resistance to citrus psorosis virus (CPsV) necessitates a transgenic approach for the development of CPsV-resistant citrus. To evaluate the feasibility of conferring resistance to a non-transgenic scion, we have assembled citrus plants by grafting combining a non-transgenic Sweet Orange as scion, CPsV-resistant transgenic Sweet Orange lines expressing intron-hairpin (ihp) RNA derived from the viral coat protein (ihpCP) as interstock, and a non-transgenic citrus as rootstock. We demonstrated that ihpCP-transcripts translocate through the graft from interstock to scion, triggering the silencing of coat protein mRNA target. Two independent CPsV challenge assays showed that expression of ihpCP in the interstock provides resistance against CPsV in the interstock, and different levels of protection in the non-tg scion, depending of the virus delivery site. These results indicated that grafting is a promising biotechnological alternative to protect woody plants against virus infections in vegetative propagated plants. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6776 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA67762021-03-08T14:27:09Z Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion de Francesco, Agustina Simeone, Melina Gomez, Claudio Andres Costa, Norma Beatriz Garcia, Maria Laura Citrus Naranja Dulce Plantas Transgénicas Resistencia a la Enfermedad Virus de las Plantas Sweet Oranges Transgenic Plants Disease Resistance Plant Viruses Virus de la Psoriasis de los Cítricos Citrus Psorosis Virus The lack of naturally occurring resistance to citrus psorosis virus (CPsV) necessitates a transgenic approach for the development of CPsV-resistant citrus. To evaluate the feasibility of conferring resistance to a non-transgenic scion, we have assembled citrus plants by grafting combining a non-transgenic Sweet Orange as scion, CPsV-resistant transgenic Sweet Orange lines expressing intron-hairpin (ihp) RNA derived from the viral coat protein (ihpCP) as interstock, and a non-transgenic citrus as rootstock. We demonstrated that ihpCP-transcripts translocate through the graft from interstock to scion, triggering the silencing of coat protein mRNA target. Two independent CPsV challenge assays showed that expression of ihpCP in the interstock provides resistance against CPsV in the interstock, and different levels of protection in the non-tg scion, depending of the virus delivery site. These results indicated that grafting is a promising biotechnological alternative to protect woody plants against virus infections in vegetative propagated plants. EEA Concordia Fil: de Francesco, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. University of California at Riverside. Department of Microbiology and Plant Pathology; Estados Unidos Fil: Simeone, M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Gomez, Claudio Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Costa, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina 2020-02-17T12:59:35Z 2020-02-17T12:59:35Z 2020-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6776 http://link.springer.com/10.1007/s11248-020-00191-1 0962-8819 1573-9368 https://doi.org/10.1007/s11248-020-00191-1 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Transgenic Research : 1-14 (January 2020) |
| spellingShingle | Citrus Naranja Dulce Plantas Transgénicas Resistencia a la Enfermedad Virus de las Plantas Sweet Oranges Transgenic Plants Disease Resistance Plant Viruses Virus de la Psoriasis de los Cítricos Citrus Psorosis Virus de Francesco, Agustina Simeone, Melina Gomez, Claudio Andres Costa, Norma Beatriz Garcia, Maria Laura Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion |
| title | Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion |
| title_full | Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion |
| title_fullStr | Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion |
| title_full_unstemmed | Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion |
| title_short | Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion |
| title_sort | transgenic sweet orange expressing hairpin cp mrna in the interstock confers tolerance to citrus psorosis virus in the non transgenic scion |
| topic | Citrus Naranja Dulce Plantas Transgénicas Resistencia a la Enfermedad Virus de las Plantas Sweet Oranges Transgenic Plants Disease Resistance Plant Viruses Virus de la Psoriasis de los Cítricos Citrus Psorosis Virus |
| url | http://hdl.handle.net/20.500.12123/6776 http://link.springer.com/10.1007/s11248-020-00191-1 https://doi.org/10.1007/s11248-020-00191-1 |
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