Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages
The aim of this study was to evaluate and compare the ability to adhere/internalize, persist, and induce damage in mammary epithelial cells (MAC-T) of two Staphylococcus aureus strains with different adaptation genotypes (low and high) to the bovine mammary gland (MG). Also, the phagocytic and bacte...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/6764 https://www.sciencedirect.com/science/article/pii/S0882401019318546 https://doi.org/10.1016/j.micpath.2020.104017 |
| _version_ | 1855483875545841664 |
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| author | Sacco, Sofía Clara Velázquez, Natalia S. Renna, María Sol Beccaria, Camila Baravalle, Celina Pereyra, Elizabet Amanda Lorena Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet |
| author_browse | Baravalle, Celina Beccaria, Camila Calvinho, Luis Fernando Dallard, Bibiana Elisabet Monecke, Stefan Pereyra, Elizabet Amanda Lorena Renna, María Sol Sacco, Sofía Clara Velázquez, Natalia S. |
| author_facet | Sacco, Sofía Clara Velázquez, Natalia S. Renna, María Sol Beccaria, Camila Baravalle, Celina Pereyra, Elizabet Amanda Lorena Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet |
| author_sort | Sacco, Sofía Clara |
| collection | INTA Digital |
| description | The aim of this study was to evaluate and compare the ability to adhere/internalize, persist, and induce damage in mammary epithelial cells (MAC-T) of two Staphylococcus aureus strains with different adaptation genotypes (low and high) to the bovine mammary gland (MG). Also, the phagocytic and bactericidal capacity induced after the interaction between macrophages, isolated from mammary secretion, of both S. aureus strains was evaluated. Two isolates (designated 806 and 5011) from bovine intramammary infection (IMI) harboring genes involved in adherence and biofilm production, belonging to different capsular polysaccharide (CP) type, accessory gene regulator (agr) group, pulsotype (PT) and sequence type/clonal complex (ST/CC). Strains 806 and 5011 were associated with low (nonpersistent-NP) and high (persistent-P) adaptation to the MG, respectively. Strain 5011 (P), agr group I, cap8 positive and strong biofilm producer showed higher capacity to adhere/internalize in MAC-T compared with strain 806 (NP), characterized as agr group II, cap5 positive and weak biofilm producer. Strain 5011(P) could be recovered from MAC-T lysates up to 72 h pi; while strain 806 (NP) could be recovered only at 4 h pi. Strain 5011 (P) showed greater capacity to induce apoptosis compared with strain 806 (NP) at 4, 24 and 48 h pi. Macrophages infected with strain 5011 (P) showed a greater phagocytic capacity and higher percentage of intracellular reactive oxygen species (ROS) production than strain 806 (NP). No viable bacteria were isolated from macrophages lysates stimulated with any of the S. aureus strains at 2, 4, 8 and 24 h pi. The knowledge of the molecular profile of the S. aureus strains causing bovine mastitis in a herd could become a tool to expose the most prevalent virulence gene patterns and advance in the elucidation of the pathogenesis of chronic mastitis. |
| format | Artículo |
| id | INTA6764 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA67642020-02-14T12:39:06Z Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages Sacco, Sofía Clara Velázquez, Natalia S. Renna, María Sol Beccaria, Camila Baravalle, Celina Pereyra, Elizabet Amanda Lorena Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet Staphylococcus aureus Genotipos Enfermedades de los Animales Ganado Bovino Mastítis Bovina Macrofagos Genotypes Animal Diseases Cattle Bovine Mastitis Macrophages The aim of this study was to evaluate and compare the ability to adhere/internalize, persist, and induce damage in mammary epithelial cells (MAC-T) of two Staphylococcus aureus strains with different adaptation genotypes (low and high) to the bovine mammary gland (MG). Also, the phagocytic and bactericidal capacity induced after the interaction between macrophages, isolated from mammary secretion, of both S. aureus strains was evaluated. Two isolates (designated 806 and 5011) from bovine intramammary infection (IMI) harboring genes involved in adherence and biofilm production, belonging to different capsular polysaccharide (CP) type, accessory gene regulator (agr) group, pulsotype (PT) and sequence type/clonal complex (ST/CC). Strains 806 and 5011 were associated with low (nonpersistent-NP) and high (persistent-P) adaptation to the MG, respectively. Strain 5011 (P), agr group I, cap8 positive and strong biofilm producer showed higher capacity to adhere/internalize in MAC-T compared with strain 806 (NP), characterized as agr group II, cap5 positive and weak biofilm producer. Strain 5011(P) could be recovered from MAC-T lysates up to 72 h pi; while strain 806 (NP) could be recovered only at 4 h pi. Strain 5011 (P) showed greater capacity to induce apoptosis compared with strain 806 (NP) at 4, 24 and 48 h pi. Macrophages infected with strain 5011 (P) showed a greater phagocytic capacity and higher percentage of intracellular reactive oxygen species (ROS) production than strain 806 (NP). No viable bacteria were isolated from macrophages lysates stimulated with any of the S. aureus strains at 2, 4, 8 and 24 h pi. The knowledge of the molecular profile of the S. aureus strains causing bovine mastitis in a herd could become a tool to expose the most prevalent virulence gene patterns and advance in the elucidation of the pathogenesis of chronic mastitis. EEA Rafaela Fil: Sacco, Sofía Clara. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Velázquez, Natalia S. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Renna, María Sol. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Beccaría, Camila. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Baravalle, Celina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Pereyra, Elizabet Amanda Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Monecke, Stefan. Institute for Medical Microbiology and Hygiene; Alemania. Alere Technologies GmbH; Alemania Fil: Calvinho, Luis Fernando. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Clínicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina 2020-02-14T12:36:40Z 2020-02-14T12:36:40Z 2020-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6764 https://www.sciencedirect.com/science/article/pii/S0882401019318546 0882-4010 1096-1208 https://doi.org/10.1016/j.micpath.2020.104017 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Microbial Pathogenesis 142 : 104017 (2020) |
| spellingShingle | Staphylococcus aureus Genotipos Enfermedades de los Animales Ganado Bovino Mastítis Bovina Macrofagos Genotypes Animal Diseases Cattle Bovine Mastitis Macrophages Sacco, Sofía Clara Velázquez, Natalia S. Renna, María Sol Beccaria, Camila Baravalle, Celina Pereyra, Elizabet Amanda Lorena Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages |
| title | Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages |
| title_full | Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages |
| title_fullStr | Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages |
| title_full_unstemmed | Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages |
| title_short | Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages |
| title_sort | capacity of two staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages |
| topic | Staphylococcus aureus Genotipos Enfermedades de los Animales Ganado Bovino Mastítis Bovina Macrofagos Genotypes Animal Diseases Cattle Bovine Mastitis Macrophages |
| url | http://hdl.handle.net/20.500.12123/6764 https://www.sciencedirect.com/science/article/pii/S0882401019318546 https://doi.org/10.1016/j.micpath.2020.104017 |
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