Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
Kainic acid (KA) is an analog of the neurotransmitter glutamate and is widely used as an excitotoxic agent to lesion spinal cord networks, thus, providing an interesting model to learn basic mechanisms of spinal cord injury. The present work was aimed to evaluate motor and sensory performance of rat...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Español |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/6708 https://www.sciencedirect.com/science/article/pii/S0161813X15000820 https://doi.org/10.1016/j.neuro.2015.05.006 |
| _version_ | 1855035725360136192 |
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| author | Nishida, Fabián Zanuzzi, Carolina Natalia Martinez, Agustin Barbeito, Claudio Gustavo Portiansky, Enrique Leo |
| author_browse | Barbeito, Claudio Gustavo Martinez, Agustin Nishida, Fabián Portiansky, Enrique Leo Zanuzzi, Carolina Natalia |
| author_facet | Nishida, Fabián Zanuzzi, Carolina Natalia Martinez, Agustin Barbeito, Claudio Gustavo Portiansky, Enrique Leo |
| author_sort | Nishida, Fabián |
| collection | INTA Digital |
| description | Kainic acid (KA) is an analog of the neurotransmitter glutamate and is widely used as an excitotoxic agent to lesion spinal cord networks, thus, providing an interesting model to learn basic mechanisms of spinal cord injury. The present work was aimed to evaluate motor and sensory performance of rats and analyze morphometric parameters of spinal cord neurons after KA injection. Animals were injected either with 0.75, 1 or 1.25 mM of KA at the C5 segment of the cervical spinal cord. Motor and sensory
performance of the rats were evaluate at day 0 (before injection) and at days 1, 2, 3 and 7 post-injection (pi) and compared with those of saline-treated and non-operated animals. Animals were sacrificed at each time point for morphometric and histopathological analysis and compared among groups. All KAtreated animals showed a significant impairment at the motor and sensory tests for the ipsilateral forelimb in a concentration-dependent manner in comparison to saline-treated and non-operated animals. Neuronal cell count showed a significant loss of neurons at C4, C5 and C6 cervical segments when compared with those of saline-treated and non-operated animals. The contralateral side of the cervical segments in KA-treated rats remained unchanged. Some improvement at the motor and sensory tests was observed in animals injected with 0.75 and 1 mM KA. Moreover, a mild increase in the neuronal
count of the damaged segments was also recorded. The improvement recorded in the motor and sensory tests by day 7 pi may be a consequence of a neuron repairing mechanism triggered soon after the KA excitotoxic effect. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6708 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Español |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA67082020-02-10T14:33:52Z Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord Nishida, Fabián Zanuzzi, Carolina Natalia Martinez, Agustin Barbeito, Claudio Gustavo Portiansky, Enrique Leo Sanidad Animal Rata Animal Health Rats Ácido Kaínico (KA) Kainic acid (KA) is an analog of the neurotransmitter glutamate and is widely used as an excitotoxic agent to lesion spinal cord networks, thus, providing an interesting model to learn basic mechanisms of spinal cord injury. The present work was aimed to evaluate motor and sensory performance of rats and analyze morphometric parameters of spinal cord neurons after KA injection. Animals were injected either with 0.75, 1 or 1.25 mM of KA at the C5 segment of the cervical spinal cord. Motor and sensory performance of the rats were evaluate at day 0 (before injection) and at days 1, 2, 3 and 7 post-injection (pi) and compared with those of saline-treated and non-operated animals. Animals were sacrificed at each time point for morphometric and histopathological analysis and compared among groups. All KAtreated animals showed a significant impairment at the motor and sensory tests for the ipsilateral forelimb in a concentration-dependent manner in comparison to saline-treated and non-operated animals. Neuronal cell count showed a significant loss of neurons at C4, C5 and C6 cervical segments when compared with those of saline-treated and non-operated animals. The contralateral side of the cervical segments in KA-treated rats remained unchanged. Some improvement at the motor and sensory tests was observed in animals injected with 0.75 and 1 mM KA. Moreover, a mild increase in the neuronal count of the damaged segments was also recorded. The improvement recorded in the motor and sensory tests by day 7 pi may be a consequence of a neuron repairing mechanism triggered soon after the KA excitotoxic effect. Estación Experimental Agropecuaria Bariloche Fil: Nishida, Fabián. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Laboratorio de Análisis de Imágenes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zanuzzi, Carolina Natalia. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias; Argentina. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Departamento de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Agustin. Instituto Nacional de Tecnología Aropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; Argentina Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias; Argentina. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Departamento de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Laboratorio de Análisis de Imágenes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-02-10T14:19:42Z 2020-02-10T14:19:42Z 2015-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6708 https://www.sciencedirect.com/science/article/pii/S0161813X15000820 0161-813X https://doi.org/10.1016/j.neuro.2015.05.006 spa info:eu-repo/semantics/restrictedAccess application/pdf Elsevier NeuroToxicology 49 : 68–78 (2015) |
| spellingShingle | Sanidad Animal Rata Animal Health Rats Ácido Kaínico (KA) Nishida, Fabián Zanuzzi, Carolina Natalia Martinez, Agustin Barbeito, Claudio Gustavo Portiansky, Enrique Leo Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord |
| title | Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord |
| title_full | Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord |
| title_fullStr | Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord |
| title_full_unstemmed | Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord |
| title_short | Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord |
| title_sort | functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord |
| topic | Sanidad Animal Rata Animal Health Rats Ácido Kaínico (KA) |
| url | http://hdl.handle.net/20.500.12123/6708 https://www.sciencedirect.com/science/article/pii/S0161813X15000820 https://doi.org/10.1016/j.neuro.2015.05.006 |
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