Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd
In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Instituto Nacional de Investigaciones Forestales, Agrícola y Pecuarias, México
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/6702 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias |
| _version_ | 1855035723135057920 |
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| author | Macias Rioseco, Melissa Caffarena, Rubén Fraga, Martín Silveira, Caroline Giannitti, Federico Canton, German Jose Hecker, Yanina Suanes, Alejandra Riet Correa, Franklin |
| author_browse | Caffarena, Rubén Canton, German Jose Fraga, Martín Giannitti, Federico Hecker, Yanina Macias Rioseco, Melissa Riet Correa, Franklin Silveira, Caroline Suanes, Alejandra |
| author_facet | Macias Rioseco, Melissa Caffarena, Rubén Fraga, Martín Silveira, Caroline Giannitti, Federico Canton, German Jose Hecker, Yanina Suanes, Alejandra Riet Correa, Franklin |
| author_sort | Macias Rioseco, Melissa |
| collection | INTA Digital |
| description | In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous epicarditis and peritonitis, and neutrophilic bronchopneumonia. Campylobacter fetus subsp. venerealis was detected by direct immunofluorescence, isolated and identified by PCR and sequencing of the 16S rDNA in the abomasal fluid and/or lung. Histologic examination of two other fetuses revealed nonsuppurative necrotizing encephalitis, lymphohistiocytic myositis and myocarditis, and lymphocytic interstitial nephritis. In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. Antibodies against N. caninum were detected by indirect immunofluorescence in 10 of 27 cows, with titers ranging from 1/200 to 1/3200. The results indicate that two abortigenic microorganisms may coexist and cause contemporaneous abortion in a herd. It is relevant to highlight the importance of performing multiple diagnostic tests in various aborted dams and fetuses from the same herd for the etiologic confirmation of bovine abortion syndrome |
| format | info:ar-repo/semantics/artículo |
| id | INTA6702 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Instituto Nacional de Investigaciones Forestales, Agrícola y Pecuarias, México |
| publisherStr | Instituto Nacional de Investigaciones Forestales, Agrícola y Pecuarias, México |
| record_format | dspace |
| spelling | INTA67022020-02-10T14:03:28Z Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd Macias Rioseco, Melissa Caffarena, Rubén Fraga, Martín Silveira, Caroline Giannitti, Federico Canton, German Jose Hecker, Yanina Suanes, Alejandra Riet Correa, Franklin Aborto Campylobacter Fetus Neospora Caninum Diagnóstico Ganado Bovino Abortion Diagnosis Cattle In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous epicarditis and peritonitis, and neutrophilic bronchopneumonia. Campylobacter fetus subsp. venerealis was detected by direct immunofluorescence, isolated and identified by PCR and sequencing of the 16S rDNA in the abomasal fluid and/or lung. Histologic examination of two other fetuses revealed nonsuppurative necrotizing encephalitis, lymphohistiocytic myositis and myocarditis, and lymphocytic interstitial nephritis. In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. Antibodies against N. caninum were detected by indirect immunofluorescence in 10 of 27 cows, with titers ranging from 1/200 to 1/3200. The results indicate that two abortigenic microorganisms may coexist and cause contemporaneous abortion in a herd. It is relevant to highlight the importance of performing multiple diagnostic tests in various aborted dams and fetuses from the same herd for the etiologic confirmation of bovine abortion syndrome En noviembre de 2015 ocurrió un brote de abortos en un hato lechero comercial de 650 vacas Holstein en el departamento de Florida, Uruguay. Cuarenta y cinco (45) vacas abortaron en un lapso de 3 semanas. Cinco fetos fueron sometidos a un examen patológico macro y microscópico y a pruebas microbiológicas. Un feto tenía epicarditis fibrinosa y peritonitis, así como bronconeumonía neutrofílica. Se detectó Campylobacter fetus subesp. venerealis utilizando inmunofluorescencia directa; se lo aisló e identificó mediante PCR y secuenciación del 16S rDNA en el líquido abomasal y en el pulmón. El examen histológico de otros dos fetos reveló encefalitis necrotizante no supurativa, miositis linfohistiocítica y miocarditis, y nefritis linfocítica intersticial. En estos fetos se detectó intralesionalmente el antígeno de N. caninum mediante análisis inmunohistoquímico, y se amplificó el ADN de N. caninum mediante PCR en tejido cerebral fijado con formalina y embebido en parafina. Se detectaron anticuerpos contra N. caninum mediante inmunofluorescencia indirecta en 10 de 27 vacas, con títulos de entre 1/200 y 1/3200. Los resultados indican que dos microorganismos abortígenos pueden coexistir y provocar abortos contemporáneos en un hato. Subrayamos la importancia de realizar pruebas diagnósticas múltiples en diversas madres abortadas y fetos del mismo hato para obtener una confirmación etiológica del síndrome de aborto bovino. EEA Balcarce Fil: Macías-Rioseco, Melissa. Instituto Nacional de Investigación Agropecuaria; Uruguay Fil: Caffarena, Rubén D. Instituto Nacional de Investigación Agropecuaria; Uruguay. Universidad de la República, Facultad de Veterinaria; Uruguay Fil: Fraga, Martín. Instituto Nacional de Investigación Agropecuaria; Uruguay Fil: Silveira, Caroline. Instituto Nacional de Investigación Agropecuaria; Uruguay Fil: Giannitti, Federico. Instituto Nacional de Investigación Agropecuaria; Uruguay. University of Minnesota, Veterinary Population Medicine Departament; Usa Fil: Cantón, Germán. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Hecker, Yanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Suanes, Alejandra. Ministerio de Ganadería Agricultura y Pesca. Montevideo. Dirección de Laboratorios Veterinarios; Uruguay. Fil: Riet-Correa, Franklin. Instituto Nacional de Investigación Agropecuaria; Uruguay 2020-02-10T13:40:20Z 2020-02-10T13:40:20Z 2019-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6702 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias 2448-6698 2007-1124 (Online) eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Instituto Nacional de Investigaciones Forestales, Agrícola y Pecuarias, México Revista mexicana de ciencias pecuarias 10 (4) :1054-1063. (2019) |
| spellingShingle | Aborto Campylobacter Fetus Neospora Caninum Diagnóstico Ganado Bovino Abortion Diagnosis Cattle Macias Rioseco, Melissa Caffarena, Rubén Fraga, Martín Silveira, Caroline Giannitti, Federico Canton, German Jose Hecker, Yanina Suanes, Alejandra Riet Correa, Franklin Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd |
| title | Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd |
| title_full | Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd |
| title_fullStr | Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd |
| title_full_unstemmed | Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd |
| title_short | Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd |
| title_sort | abortion outbreak caused by campylobacter fetus subspecies venerealis and neospora caninum in a bovine dairy herd |
| topic | Aborto Campylobacter Fetus Neospora Caninum Diagnóstico Ganado Bovino Abortion Diagnosis Cattle |
| url | http://hdl.handle.net/20.500.12123/6702 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias |
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