Molecular characterization of pestiviruses in fetal bovine sera originating from Argentina: evidence of circulation of HoBi-like viruses

Serological evidence suggests that HoBi-like viruses, an emerging species within the Pestivirus genus of the Flaviviridae family, are in circulation in Argentina. While HoBi-like viruses were first isolated from Brazilian fetal bovine serum (FBS), no survey of Argentine FBS has been conducted. There...

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Main Authors: Pecora, Andrea, Perez Aguirreburiald, Maria Sol, Ridpath, Julia, Dus Santos, Maria Jose
Format: Artículo
Language:Inglés
Published: Frontiers Media 2020
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/6691
https://www.frontiersin.org/articles/10.3389/fvets.2019.00359/full
https://doi.org/10.3389/fvets.2019.00359
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author Pecora, Andrea
Perez Aguirreburiald, Maria Sol
Ridpath, Julia
Dus Santos, Maria Jose
author_browse Dus Santos, Maria Jose
Pecora, Andrea
Perez Aguirreburiald, Maria Sol
Ridpath, Julia
author_facet Pecora, Andrea
Perez Aguirreburiald, Maria Sol
Ridpath, Julia
Dus Santos, Maria Jose
author_sort Pecora, Andrea
collection INTA Digital
description Serological evidence suggests that HoBi-like viruses, an emerging species within the Pestivirus genus of the Flaviviridae family, are in circulation in Argentina. While HoBi-like viruses were first isolated from Brazilian fetal bovine serum (FBS), no survey of Argentine FBS has been conducted. Therefore, 124 local samples of non-irradiated FBS originating from Argentina were surveyed for the presence of pestiviruses using RT-PCR. Amplicons from pestivirus positive samples were genotyped. Four samples were positive for HoBi virus-specific RT-PCR, while the BVDV-positive samples (n = 45) were classified as BVDV-1b (82.2%), BVDV-1a (13.3%), and BVDV-2 (4.5%). Virus isolation and serological profile assessment were performed for the four HoBi-positive FBS lots. These results confirm the circulation of HoBi-like virus in some regions of the Argentinean territory, highlighting the need to review the diagnostic techniques currently used in the clinical cases suspected of BVDV and in contamination control protocols for adventitious agents in cells and biotechnological products.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
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spelling INTA66912020-01-16T15:33:41Z Molecular characterization of pestiviruses in fetal bovine sera originating from Argentina: evidence of circulation of HoBi-like viruses Pecora, Andrea Perez Aguirreburiald, Maria Sol Ridpath, Julia Dus Santos, Maria Jose Pestivirus Técnicas de Diagnosis Encuestas Argentina Biología Molecular Pestivirus de la Diarrea Bovina Diagnostic Techniques Surveys Molecular Biology Bovine Diarrhoea Pestivirus Molecular Characterization Caracterización Molecular Serological evidence suggests that HoBi-like viruses, an emerging species within the Pestivirus genus of the Flaviviridae family, are in circulation in Argentina. While HoBi-like viruses were first isolated from Brazilian fetal bovine serum (FBS), no survey of Argentine FBS has been conducted. Therefore, 124 local samples of non-irradiated FBS originating from Argentina were surveyed for the presence of pestiviruses using RT-PCR. Amplicons from pestivirus positive samples were genotyped. Four samples were positive for HoBi virus-specific RT-PCR, while the BVDV-positive samples (n = 45) were classified as BVDV-1b (82.2%), BVDV-1a (13.3%), and BVDV-2 (4.5%). Virus isolation and serological profile assessment were performed for the four HoBi-positive FBS lots. These results confirm the circulation of HoBi-like virus in some regions of the Argentinean territory, highlighting the need to review the diagnostic techniques currently used in the clinical cases suspected of BVDV and in contamination control protocols for adventitious agents in cells and biotechnological products. Instituto de Virología Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. University of Minnesota. Center for Animal Health and Food Safety; Estados Unidos Fil: Ridpath, Julia. Ridpath Consulting; Estados Unidos Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-01-16T15:26:42Z 2020-01-16T15:26:42Z 2019-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6691 https://www.frontiersin.org/articles/10.3389/fvets.2019.00359/full 2297-1769 https://doi.org/10.3389/fvets.2019.00359 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in veterinary sciences 6 : 359. (October 2019)
spellingShingle Pestivirus
Técnicas de Diagnosis
Encuestas
Argentina
Biología Molecular
Pestivirus de la Diarrea Bovina
Diagnostic Techniques
Surveys
Molecular Biology
Bovine Diarrhoea Pestivirus
Molecular Characterization
Caracterización Molecular
Pecora, Andrea
Perez Aguirreburiald, Maria Sol
Ridpath, Julia
Dus Santos, Maria Jose
Molecular characterization of pestiviruses in fetal bovine sera originating from Argentina: evidence of circulation of HoBi-like viruses
title Molecular characterization of pestiviruses in fetal bovine sera originating from Argentina: evidence of circulation of HoBi-like viruses
title_full Molecular characterization of pestiviruses in fetal bovine sera originating from Argentina: evidence of circulation of HoBi-like viruses
title_fullStr Molecular characterization of pestiviruses in fetal bovine sera originating from Argentina: evidence of circulation of HoBi-like viruses
title_full_unstemmed Molecular characterization of pestiviruses in fetal bovine sera originating from Argentina: evidence of circulation of HoBi-like viruses
title_short Molecular characterization of pestiviruses in fetal bovine sera originating from Argentina: evidence of circulation of HoBi-like viruses
title_sort molecular characterization of pestiviruses in fetal bovine sera originating from argentina evidence of circulation of hobi like viruses
topic Pestivirus
Técnicas de Diagnosis
Encuestas
Argentina
Biología Molecular
Pestivirus de la Diarrea Bovina
Diagnostic Techniques
Surveys
Molecular Biology
Bovine Diarrhoea Pestivirus
Molecular Characterization
Caracterización Molecular
url http://hdl.handle.net/20.500.12123/6691
https://www.frontiersin.org/articles/10.3389/fvets.2019.00359/full
https://doi.org/10.3389/fvets.2019.00359
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