Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and n...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Springer
2020
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/6685 https://link.springer.com/article/10.1007%2Fs00425-019-03178-3 https://doi.org/10.1007/s00425-019-03178-3 |
| _version_ | 1855035720414003200 |
|---|---|
| author | Acevedo, Raúl Maximiliano Avico, Edgardo H. Gonzalez, Sergio Alberto Rodrigues Salvador, Acácio Rivarola, Maximo Lisandro Paniego, Norma Beatriz Nunes Nesi, Adriano Ruiz, Oscar Adolfo Sansberro, Pedro Alfonso |
| author_browse | Acevedo, Raúl Maximiliano Avico, Edgardo H. Gonzalez, Sergio Alberto Nunes Nesi, Adriano Paniego, Norma Beatriz Rivarola, Maximo Lisandro Rodrigues Salvador, Acácio Ruiz, Oscar Adolfo Sansberro, Pedro Alfonso |
| author_facet | Acevedo, Raúl Maximiliano Avico, Edgardo H. Gonzalez, Sergio Alberto Rodrigues Salvador, Acácio Rivarola, Maximo Lisandro Paniego, Norma Beatriz Nunes Nesi, Adriano Ruiz, Oscar Adolfo Sansberro, Pedro Alfonso |
| author_sort | Acevedo, Raúl Maximiliano |
| collection | INTA Digital |
| description | Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6685 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA66852020-04-07T17:17:13Z Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves Acevedo, Raúl Maximiliano Avico, Edgardo H. Gonzalez, Sergio Alberto Rodrigues Salvador, Acácio Rivarola, Maximo Lisandro Paniego, Norma Beatriz Nunes Nesi, Adriano Ruiz, Oscar Adolfo Sansberro, Pedro Alfonso Abiotic Stress Transcription Plant Response Drought Resistance Plant Water Relations Estrés Abiótico Ilex Paraguariensis Transcripción Mate Respuesta de la Planta Resistencia a la Sequía Relaciones Planta Agua Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species. Instituto de Biotecnología Fil: Acevedo, Raúl M. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste. Facultad de Ciencias Agrarias. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: Avico, Edgardo H. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste. Facultad de Ciencias Agrarias. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rodrigues Salvador, Acácio. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; Brasil Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nunes Nesi, Adriano. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; Brasil Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina 2020-01-16T11:33:33Z 2020-01-16T11:33:33Z 2019-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6685 https://link.springer.com/article/10.1007%2Fs00425-019-03178-3 0032-0935 1432-2048 (Online) https://doi.org/10.1007/s00425-019-03178-3 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Planta 250 (2) : 445-462. (August 2019) |
| spellingShingle | Abiotic Stress Transcription Plant Response Drought Resistance Plant Water Relations Estrés Abiótico Ilex Paraguariensis Transcripción Mate Respuesta de la Planta Resistencia a la Sequía Relaciones Planta Agua Acevedo, Raúl Maximiliano Avico, Edgardo H. Gonzalez, Sergio Alberto Rodrigues Salvador, Acácio Rivarola, Maximo Lisandro Paniego, Norma Beatriz Nunes Nesi, Adriano Ruiz, Oscar Adolfo Sansberro, Pedro Alfonso Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves |
| title | Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves |
| title_full | Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves |
| title_fullStr | Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves |
| title_full_unstemmed | Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves |
| title_short | Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves |
| title_sort | transcript and metabolic adjustments triggered by drought in ilex paraguariensis leaves |
| topic | Abiotic Stress Transcription Plant Response Drought Resistance Plant Water Relations Estrés Abiótico Ilex Paraguariensis Transcripción Mate Respuesta de la Planta Resistencia a la Sequía Relaciones Planta Agua |
| url | http://hdl.handle.net/20.500.12123/6685 https://link.springer.com/article/10.1007%2Fs00425-019-03178-3 https://doi.org/10.1007/s00425-019-03178-3 |
| work_keys_str_mv | AT acevedoraulmaximiliano transcriptandmetabolicadjustmentstriggeredbydroughtinilexparaguariensisleaves AT avicoedgardoh transcriptandmetabolicadjustmentstriggeredbydroughtinilexparaguariensisleaves AT gonzalezsergioalberto transcriptandmetabolicadjustmentstriggeredbydroughtinilexparaguariensisleaves AT rodriguessalvadoracacio transcriptandmetabolicadjustmentstriggeredbydroughtinilexparaguariensisleaves AT rivarolamaximolisandro transcriptandmetabolicadjustmentstriggeredbydroughtinilexparaguariensisleaves AT paniegonormabeatriz transcriptandmetabolicadjustmentstriggeredbydroughtinilexparaguariensisleaves AT nunesnesiadriano transcriptandmetabolicadjustmentstriggeredbydroughtinilexparaguariensisleaves AT ruizoscaradolfo transcriptandmetabolicadjustmentstriggeredbydroughtinilexparaguariensisleaves AT sansberropedroalfonso transcriptandmetabolicadjustmentstriggeredbydroughtinilexparaguariensisleaves |