Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil
A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina. Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium. Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Microbiology Society
2020
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/6684 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003686#tab2 https://doi.org/10.1099/ijsem.0.003686 |
| _version_ | 1855483861176156160 |
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| author | Ghio, Silvina Sauka, Diego Hernan Ferrari, Alejandro E. Piccinni, Florencia Elizabeth Ontañon, Ornella Mailén Campos, Eleonora |
| author_browse | Campos, Eleonora Ferrari, Alejandro E. Ghio, Silvina Ontañon, Ornella Mailén Piccinni, Florencia Elizabeth Sauka, Diego Hernan |
| author_facet | Ghio, Silvina Sauka, Diego Hernan Ferrari, Alejandro E. Piccinni, Florencia Elizabeth Ontañon, Ornella Mailén Campos, Eleonora |
| author_sort | Ghio, Silvina |
| collection | INTA Digital |
| description | A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina. Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium. Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %). Chemotaxonomic analysis revealed that strain A59Tpossesses meso-diaminopimelic acid in the cell wall. It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %). According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %). However, phylogenetic studies based on the housekeeping gyrB gene placed A59T in a separate branch from all other related type strains. Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species. The DNA G+C content was estimated as 46.09 mol%, based on genome sequencing. On the basis of these results, A59T represents a new species of the genus Paenibacillus, and we propose the name Paenibacillusxylanivorans sp. nov. The type strain is A59T (=DSM 107920T=NCIMB 15123T). |
| format | Artículo |
| id | INTA6684 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Microbiology Society |
| publisherStr | Microbiology Society |
| record_format | dspace |
| spelling | INTA66842020-01-15T15:45:30Z Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil Ghio, Silvina Sauka, Diego Hernan Ferrari, Alejandro E. Piccinni, Florencia Elizabeth Ontañon, Ornella Mailén Campos, Eleonora Forest Soils Suelo Forestal Paenibacillus Bacteria Paenibacillus Xylanivorans A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina. Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium. Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %). Chemotaxonomic analysis revealed that strain A59Tpossesses meso-diaminopimelic acid in the cell wall. It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C15 : 0 (35.1 %), with a moderate amount of C16 : 0 (6.9 %). According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638T (99.1 %) and is closely related to Paenibacillus tundrae A10bT (98.8 %). However, phylogenetic studies based on the housekeeping gyrB gene placed A59T in a separate branch from all other related type strains. Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species. The DNA G+C content was estimated as 46.09 mol%, based on genome sequencing. On the basis of these results, A59T represents a new species of the genus Paenibacillus, and we propose the name Paenibacillusxylanivorans sp. nov. The type strain is A59T (=DSM 107920T=NCIMB 15123T). Instituto de Biotecnología Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ferrari, Alejandro E. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo; Argentina Fil: Piccinni, Florencia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-01-15T15:35:55Z 2020-01-15T15:35:55Z 2019-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6684 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003686#tab2 1466-5026 1466-5034 (Online) https://doi.org/10.1099/ijsem.0.003686 eng info:eu-repograntAgreement/INTA/PNAIyAV/1130034/AR./Desarrollo de procesos para la transformación de biomasa en bioenergía. info:eu-repo/semantics/restrictedAccess application/pdf Microbiology Society International journal of systematic and evolutionary microbiology 69 (12) : 3818-3823. (December 2019) |
| spellingShingle | Forest Soils Suelo Forestal Paenibacillus Bacteria Paenibacillus Xylanivorans Ghio, Silvina Sauka, Diego Hernan Ferrari, Alejandro E. Piccinni, Florencia Elizabeth Ontañon, Ornella Mailén Campos, Eleonora Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil |
| title | Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil |
| title_full | Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil |
| title_fullStr | Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil |
| title_full_unstemmed | Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil |
| title_short | Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil |
| title_sort | paenibacillus xylanivorans sp nov a xylan degrading bacterium isolated from decaying forest soil |
| topic | Forest Soils Suelo Forestal Paenibacillus Bacteria Paenibacillus Xylanivorans |
| url | http://hdl.handle.net/20.500.12123/6684 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003686#tab2 https://doi.org/10.1099/ijsem.0.003686 |
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