Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress
In this study, we characterized the role of Rv2617c in the virulence of Mycobacterium tuberculosis. Rv2617c is a protein of unknown function unique to M. tuberculosis complex (MTC) and Mycobacterium leprae. In vitro, this protein interacts with the virulence factor P36 (also named Erp) and KdpF, a p...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Taylor & Francis
2020
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/6682 https://www.tandfonline.com/doi/full/10.1080/21505594.2019.1693714 https://doi.org/10.1080/21505594.2019.1693714 |
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| author | Forrellad, Marina Andrea Vazquez, Cristina Lourdes Blanco, Federico Carlos Klepp, Laura Ines Garcia, Elizabeth Andrea Rocha, Rosana Valeria Villafañe, Luciana Maria Bigi, María Mercedes Gutierrez, Maximiliano Gabriel Bigi, Fabiana |
| author_browse | Bigi, Fabiana Bigi, María Mercedes Blanco, Federico Carlos Forrellad, Marina Andrea Garcia, Elizabeth Andrea Gutierrez, Maximiliano Gabriel Klepp, Laura Ines Rocha, Rosana Valeria Vazquez, Cristina Lourdes Villafañe, Luciana Maria |
| author_facet | Forrellad, Marina Andrea Vazquez, Cristina Lourdes Blanco, Federico Carlos Klepp, Laura Ines Garcia, Elizabeth Andrea Rocha, Rosana Valeria Villafañe, Luciana Maria Bigi, María Mercedes Gutierrez, Maximiliano Gabriel Bigi, Fabiana |
| author_sort | Forrellad, Marina Andrea |
| collection | INTA Digital |
| description | In this study, we characterized the role of Rv2617c in the virulence of Mycobacterium tuberculosis. Rv2617c is a protein of unknown function unique to M. tuberculosis complex (MTC) and Mycobacterium leprae. In vitro, this protein interacts with the virulence factor P36 (also named Erp) and KdpF, a protein linked to nitrosative stress. Here, we showed that knockout of the Rv2617c gene in M. tuberculosis CDC1551 reduced the replication of the pathogen in a mouse model of infection and favored the trafficking of mycobacteria to phagolysosomes. We also demonstrated that Rv2617c and P36 are required for resistance to in vitro hydrogen peroxide treatment in M. tuberculosis and Mycobacterium bovis, respectively. These findings indicate Rv2617c and P36 act in concert to prevent bacterial damage upon oxidative stress. |
| format | Artículo |
| id | INTA6682 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA66822021-12-09T10:43:16Z Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress Forrellad, Marina Andrea Vazquez, Cristina Lourdes Blanco, Federico Carlos Klepp, Laura Ines Garcia, Elizabeth Andrea Rocha, Rosana Valeria Villafañe, Luciana Maria Bigi, María Mercedes Gutierrez, Maximiliano Gabriel Bigi, Fabiana Mycobacterium Tuberculosis Estrés Oxidativo Virulencia Patogenicidad Oxidative Stress Virulence Pathogenicity Rv2617c Erp In this study, we characterized the role of Rv2617c in the virulence of Mycobacterium tuberculosis. Rv2617c is a protein of unknown function unique to M. tuberculosis complex (MTC) and Mycobacterium leprae. In vitro, this protein interacts with the virulence factor P36 (also named Erp) and KdpF, a protein linked to nitrosative stress. Here, we showed that knockout of the Rv2617c gene in M. tuberculosis CDC1551 reduced the replication of the pathogen in a mouse model of infection and favored the trafficking of mycobacteria to phagolysosomes. We also demonstrated that Rv2617c and P36 are required for resistance to in vitro hydrogen peroxide treatment in M. tuberculosis and Mycobacterium bovis, respectively. These findings indicate Rv2617c and P36 act in concert to prevent bacterial damage upon oxidative stress. Instituto de Biotecnología Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rocha, Rosana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Villafañe, Luciana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bigi, María Mercedes. Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía; Argentina Fil: Gutierrez, Maximiliano Gabriel. The Francis Crick Institute, Host-Pathogen Interactions in Tuberculosis Laboratory; Reino Unido Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de investigaciones Científicas y Tecnológicas; Argentina 2020-01-15T13:51:04Z 2020-01-15T13:51:04Z 2019-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6682 https://www.tandfonline.com/doi/full/10.1080/21505594.2019.1693714 2150-5608 (Online) 2150-5594 https://doi.org/10.1080/21505594.2019.1693714 eng info:eu-repograntAgreement/INTA/PNBIO/1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor & Francis Virulence 10 (1) : 1026-1033. (Diciembre 2019) |
| spellingShingle | Mycobacterium Tuberculosis Estrés Oxidativo Virulencia Patogenicidad Oxidative Stress Virulence Pathogenicity Rv2617c Erp Forrellad, Marina Andrea Vazquez, Cristina Lourdes Blanco, Federico Carlos Klepp, Laura Ines Garcia, Elizabeth Andrea Rocha, Rosana Valeria Villafañe, Luciana Maria Bigi, María Mercedes Gutierrez, Maximiliano Gabriel Bigi, Fabiana Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress |
| title | Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress |
| title_full | Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress |
| title_fullStr | Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress |
| title_full_unstemmed | Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress |
| title_short | Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress |
| title_sort | rv2617c and p36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress |
| topic | Mycobacterium Tuberculosis Estrés Oxidativo Virulencia Patogenicidad Oxidative Stress Virulence Pathogenicity Rv2617c Erp |
| url | http://hdl.handle.net/20.500.12123/6682 https://www.tandfonline.com/doi/full/10.1080/21505594.2019.1693714 https://doi.org/10.1080/21505594.2019.1693714 |
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