Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles
Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/6681 https://www.sciencedirect.com/science/article/pii/S0378113519301099?via%3Dihub https://doi.org/10.1016/j.vetmic.2019.108482 |
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| author | Bigi, María Mercedes Vazquez, Cristina Lourdes Castelão, Ana Beatriz C. Garcia, Elizabeth Andrea Cataldi, Angel Adrian Jackson, Mary McNeil, Michael Soria, Marcelo Abel Zumarraga, Martin Jose Cabruja, Matias Gago, Gabriela Blanco, Federico Carlos Nishibe, Christiane Almeida Junior, Nalvo Franco de Araujo, Flabio Ribeiro de Bigi, Fabiana |
| author_browse | Almeida Junior, Nalvo Franco de Araujo, Flabio Ribeiro de Bigi, Fabiana Bigi, María Mercedes Blanco, Federico Carlos Cabruja, Matias Castelão, Ana Beatriz C. Cataldi, Angel Adrian Gago, Gabriela Garcia, Elizabeth Andrea Jackson, Mary McNeil, Michael Nishibe, Christiane Soria, Marcelo Abel Vazquez, Cristina Lourdes Zumarraga, Martin Jose |
| author_facet | Bigi, María Mercedes Vazquez, Cristina Lourdes Castelão, Ana Beatriz C. Garcia, Elizabeth Andrea Cataldi, Angel Adrian Jackson, Mary McNeil, Michael Soria, Marcelo Abel Zumarraga, Martin Jose Cabruja, Matias Gago, Gabriela Blanco, Federico Carlos Nishibe, Christiane Almeida Junior, Nalvo Franco de Araujo, Flabio Ribeiro de Bigi, Fabiana |
| author_sort | Bigi, María Mercedes |
| collection | INTA Digital |
| description | Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol
dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6681 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA66812020-01-15T13:11:10Z Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles Bigi, María Mercedes Vazquez, Cristina Lourdes Castelão, Ana Beatriz C. Garcia, Elizabeth Andrea Cataldi, Angel Adrian Jackson, Mary McNeil, Michael Soria, Marcelo Abel Zumarraga, Martin Jose Cabruja, Matias Gago, Gabriela Blanco, Federico Carlos Nishibe, Christiane Almeida Junior, Nalvo Franco de Araujo, Flabio Ribeiro de Bigi, Fabiana Mycobacterium Bovis Genomas Virulencia Mutación Patogenicidad Bovina Tuberculosis Infección por Mycobacterium Bovis Vacuna Genomes Virulence Mutation Pathogenicity Bovinae Mycobacterium Bovis Infections Vaccines Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines. Instituto de Biotecnología Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Castelão, Ana Beatriz C. Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia; Brasil Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jackson, Mary. Colorado State University. Dept. of Microbiology, Immunology and Pathology; Estados Unidos Fil: McNeil, Michael. Colorado State University. Dept. of Microbiology, Immunology and Pathology; Estados Unidos Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cabruja, Matias. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Gago, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nishibe, Christiane. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; Brasil Fil: Almeida Junior, Nalvo Franco de. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; Brasil Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de investigaciones Científicas y Tecnológicas; Argentina 2020-01-15T12:59:14Z 2020-01-15T12:59:14Z 2019-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6681 https://www.sciencedirect.com/science/article/pii/S0378113519301099?via%3Dihub 0378-1135 https://doi.org/10.1016/j.vetmic.2019.108482 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary microbiology 239 : 108482. (December 2019) |
| spellingShingle | Mycobacterium Bovis Genomas Virulencia Mutación Patogenicidad Bovina Tuberculosis Infección por Mycobacterium Bovis Vacuna Genomes Virulence Mutation Pathogenicity Bovinae Mycobacterium Bovis Infections Vaccines Bigi, María Mercedes Vazquez, Cristina Lourdes Castelão, Ana Beatriz C. Garcia, Elizabeth Andrea Cataldi, Angel Adrian Jackson, Mary McNeil, Michael Soria, Marcelo Abel Zumarraga, Martin Jose Cabruja, Matias Gago, Gabriela Blanco, Federico Carlos Nishibe, Christiane Almeida Junior, Nalvo Franco de Araujo, Flabio Ribeiro de Bigi, Fabiana Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles |
| title | Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles |
| title_full | Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles |
| title_fullStr | Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles |
| title_full_unstemmed | Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles |
| title_short | Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles |
| title_sort | analysing nonsynonymous mutations between two mycobacterium bovis strains with contrasting pathogenic profiles |
| topic | Mycobacterium Bovis Genomas Virulencia Mutación Patogenicidad Bovina Tuberculosis Infección por Mycobacterium Bovis Vacuna Genomes Virulence Mutation Pathogenicity Bovinae Mycobacterium Bovis Infections Vaccines |
| url | http://hdl.handle.net/20.500.12123/6681 https://www.sciencedirect.com/science/article/pii/S0378113519301099?via%3Dihub https://doi.org/10.1016/j.vetmic.2019.108482 |
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