Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities
Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product s...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Academia Brasileira de Ciências
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/6671 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620&lng=en&nrm=iso&tlng=en http://dx.doi.org/10.1590/0001-3765201920180568 |
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| author | Caminata Landriel, Soledad Castillo, Julieta D. L. M. Taboga, Oscar Alberto Ferrarotti, Susana Alicia Gottlieb, Alexandra Marina Costa, Hernán |
| author_browse | Caminata Landriel, Soledad Castillo, Julieta D. L. M. Costa, Hernán Ferrarotti, Susana Alicia Gottlieb, Alexandra Marina Taboga, Oscar Alberto |
| author_facet | Caminata Landriel, Soledad Castillo, Julieta D. L. M. Taboga, Oscar Alberto Ferrarotti, Susana Alicia Gottlieb, Alexandra Marina Costa, Hernán |
| author_sort | Caminata Landriel, Soledad |
| collection | INTA Digital |
| description | Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6671 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Academia Brasileira de Ciências |
| publisherStr | Academia Brasileira de Ciências |
| record_format | dspace |
| spelling | INTA66712020-01-14T13:37:17Z Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities Caminata Landriel, Soledad Castillo, Julieta D. L. M. Taboga, Oscar Alberto Ferrarotti, Susana Alicia Gottlieb, Alexandra Marina Costa, Hernán Paenibacillus Glicosidasas Marcadores Genéticos Actividad Enzimática Filogenia Glycosidases Genetic Markers Enzyme Activity Phylogeny Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases. Instituto de Biotecnología Fil: Caminata Landriel, Soledad. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina Fil: Castillo, Julieta D. L. M. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina. Fil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas.Laboratorio de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-01-14T13:30:43Z 2020-01-14T13:30:43Z 2019-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6671 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620&lng=en&nrm=iso&tlng=en 1678-2690 http://dx.doi.org/10.1590/0001-3765201920180568 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências 91 (3) : e20180568. (Octubre 2019) |
| spellingShingle | Paenibacillus Glicosidasas Marcadores Genéticos Actividad Enzimática Filogenia Glycosidases Genetic Markers Enzyme Activity Phylogeny Caminata Landriel, Soledad Castillo, Julieta D. L. M. Taboga, Oscar Alberto Ferrarotti, Susana Alicia Gottlieb, Alexandra Marina Costa, Hernán Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities |
| title | Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities |
| title_full | Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities |
| title_fullStr | Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities |
| title_full_unstemmed | Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities |
| title_short | Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities |
| title_sort | molecular identification of a cyclodextrin glycosyltransferase producing microorganism and phylogenetic assessment of enzymatic activities |
| topic | Paenibacillus Glicosidasas Marcadores Genéticos Actividad Enzimática Filogenia Glycosidases Genetic Markers Enzyme Activity Phylogeny |
| url | http://hdl.handle.net/20.500.12123/6671 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620&lng=en&nrm=iso&tlng=en http://dx.doi.org/10.1590/0001-3765201920180568 |
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