Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Rea...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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The Japanese Society of Veterinary Science
2019
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| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_article http://hdl.handle.net/20.500.12123/6567 https://doi.org/10.1292/jvms.19-0214 |
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| author | Bacanelli, Gisele Olarte, Larissa C. Silva, Marcio Roberto Rodrigues, Rudielle A. Carneiro, Paulo A. M. Kannene, John B. Pasquatti, Taynara N. Takatani, Haruo Zumarraga, Martin Jose Etges, Rodrigo N. Araujo, Flabio Ribeiro de Verbisck, Newton V. |
| author_browse | Araujo, Flabio Ribeiro de Bacanelli, Gisele Carneiro, Paulo A. M. Etges, Rodrigo N. Kannene, John B. Olarte, Larissa C. Pasquatti, Taynara N. Rodrigues, Rudielle A. Silva, Marcio Roberto Takatani, Haruo Verbisck, Newton V. Zumarraga, Martin Jose |
| author_facet | Bacanelli, Gisele Olarte, Larissa C. Silva, Marcio Roberto Rodrigues, Rudielle A. Carneiro, Paulo A. M. Kannene, John B. Pasquatti, Taynara N. Takatani, Haruo Zumarraga, Martin Jose Etges, Rodrigo N. Araujo, Flabio Ribeiro de Verbisck, Newton V. |
| author_sort | Bacanelli, Gisele |
| collection | INTA Digital |
| description | In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6567 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | The Japanese Society of Veterinary Science |
| publisherStr | The Japanese Society of Veterinary Science |
| record_format | dspace |
| spelling | INTA65672019-12-26T11:09:42Z Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae Bacanelli, Gisele Olarte, Larissa C. Silva, Marcio Roberto Rodrigues, Rudielle A. Carneiro, Paulo A. M. Kannene, John B. Pasquatti, Taynara N. Takatani, Haruo Zumarraga, Martin Jose Etges, Rodrigo N. Araujo, Flabio Ribeiro de Verbisck, Newton V. Tuberculosis Infeccion por Mycobacterium bovis Tratamiento Ionizante Desorción Espectrometría Infeccion Mycobacterium tuberculosis Bovina Mycobacterium bovis Infections Ionization Treatment Desorption Spectrometry Mycobacterium tuberculosis Infections Bovinae In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. Instituto de Biotecnología Fil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; Brasil Fil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; Brasil Fil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasil Fil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; Brasil Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos Fil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil Fil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; Brasil Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Etges, Rodrigo N. Secretary of Agriculture, Livestock and Irrigation; Brasil Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil Fil: Verbisck, Newton V. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil 2019-12-26T10:52:17Z 2019-12-26T10:52:17Z 2019-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_article http://hdl.handle.net/20.500.12123/6567 1347-7439 https://doi.org/10.1292/jvms.19-0214 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf The Japanese Society of Veterinary Science The Journal of Veterinary Medical Science 81 (10) : 1400-1408 (Octubre 2019) |
| spellingShingle | Tuberculosis Infeccion por Mycobacterium bovis Tratamiento Ionizante Desorción Espectrometría Infeccion Mycobacterium tuberculosis Bovina Mycobacterium bovis Infections Ionization Treatment Desorption Spectrometry Mycobacterium tuberculosis Infections Bovinae Bacanelli, Gisele Olarte, Larissa C. Silva, Marcio Roberto Rodrigues, Rudielle A. Carneiro, Paulo A. M. Kannene, John B. Pasquatti, Taynara N. Takatani, Haruo Zumarraga, Martin Jose Etges, Rodrigo N. Araujo, Flabio Ribeiro de Verbisck, Newton V. Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
| title | Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
| title_full | Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
| title_fullStr | Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
| title_full_unstemmed | Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
| title_short | Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
| title_sort | matrix assisted laser desorption ionization time of flight mass spectrometry identification of mycobacterium bovis in bovinae |
| topic | Tuberculosis Infeccion por Mycobacterium bovis Tratamiento Ionizante Desorción Espectrometría Infeccion Mycobacterium tuberculosis Bovina Mycobacterium bovis Infections Ionization Treatment Desorption Spectrometry Mycobacterium tuberculosis Infections Bovinae |
| url | https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_article http://hdl.handle.net/20.500.12123/6567 https://doi.org/10.1292/jvms.19-0214 |
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