Detection of swainsonine-producing endophytes in Patagonian Astragalus species
Swainsonine has been identified as the toxin in legumes belonging to the genera Astragalus and Oxytropis throughout the world including China, North America, and South America. Several South American Astragalus species have been reported to contain swainsonine; however, data is lacking to support th...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/6518 https://www.sciencedirect.com/science/article/pii/S0041010119304702 https://doi.org/10.1016/j.toxicon.2019.09.020 |
| _version_ | 1855483829257502720 |
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| author | Martinez, Agustin Robles, Carlos Alejandro Roper, Jessie M. Gardner, Dale R. Neyaz, Marwah S. Joelson, Natalia Zoe Cook, Daniel |
| author_browse | Cook, Daniel Gardner, Dale R. Joelson, Natalia Zoe Martinez, Agustin Neyaz, Marwah S. Robles, Carlos Alejandro Roper, Jessie M. |
| author_facet | Martinez, Agustin Robles, Carlos Alejandro Roper, Jessie M. Gardner, Dale R. Neyaz, Marwah S. Joelson, Natalia Zoe Cook, Daniel |
| author_sort | Martinez, Agustin |
| collection | INTA Digital |
| description | Swainsonine has been identified as the toxin in legumes belonging to the genera Astragalus and Oxytropis throughout the world including China, North America, and South America. Several South American Astragalus species have been reported to contain swainsonine; however, data is lacking to support the presence of a fungal symbiont in South American Astragalus species as has been shown for North American and Chinese Astragalus and Oxytropis species. The objective of this study was to investigate several South American species that have been reported to contain swainsonine for the presence of the fungal symbiont using culturing and PCR. Swainsonine was detected in field collections of A. pehuenches, A. illinii and A. chamissonis but not A. moyanoi, which is consistent with reports of toxicity regarding these species. The symbiont Alternaria section Undifilum was detected by PCR in all three species that contained swainsonine but not in A. moyanoi. A fungal symbiont was isolated from seeds of Astragalus pehuenches and A. illinii. The isolated symbiont from both respective species produced swainsonine in vitro, and was demonstrated to belong to the genus Alternaria section Undifilum by analysis of the nuclear ribosomal DNA. It is highly likely that Alternaria section Undifilum isolates will be
associated with other South American Astragalus species that are reported to contain swainsonine. |
| format | Artículo |
| id | INTA6518 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA65182025-02-19T14:22:35Z Detection of swainsonine-producing endophytes in Patagonian Astragalus species Martinez, Agustin Robles, Carlos Alejandro Roper, Jessie M. Gardner, Dale R. Neyaz, Marwah S. Joelson, Natalia Zoe Cook, Daniel Astragalus Toxicidad Toxicidad por Ingestión Fitotoxicidad Envenenamiento Sanidad Animal Toxicity Ingestion Toxicity Phytotoxicity Poisoning Animal Health Swainsonine Región Patagónica Swainsonine has been identified as the toxin in legumes belonging to the genera Astragalus and Oxytropis throughout the world including China, North America, and South America. Several South American Astragalus species have been reported to contain swainsonine; however, data is lacking to support the presence of a fungal symbiont in South American Astragalus species as has been shown for North American and Chinese Astragalus and Oxytropis species. The objective of this study was to investigate several South American species that have been reported to contain swainsonine for the presence of the fungal symbiont using culturing and PCR. Swainsonine was detected in field collections of A. pehuenches, A. illinii and A. chamissonis but not A. moyanoi, which is consistent with reports of toxicity regarding these species. The symbiont Alternaria section Undifilum was detected by PCR in all three species that contained swainsonine but not in A. moyanoi. A fungal symbiont was isolated from seeds of Astragalus pehuenches and A. illinii. The isolated symbiont from both respective species produced swainsonine in vitro, and was demonstrated to belong to the genus Alternaria section Undifilum by analysis of the nuclear ribosomal DNA. It is highly likely that Alternaria section Undifilum isolates will be associated with other South American Astragalus species that are reported to contain swainsonine. Estación Experimental Agropecuaria Bariloche Fil: Martinez, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; Argentina Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; Argentina Fil: Roper, Jessie M. USDA. ARS Poisonous Plant Research; Estados Unidos Fil: Gardner, Dale R. USDA. ARS Poisonous Plant Research; Estados Unidos Fil: Neyaz, Marwah S. New Mexico State University. Department of Entomology, Plant Pathology and Weed Science; Mexico Fil: Joelson, Natalia Zoe. Universidad Nacional del Comahue; Argentina Fil: Cook, Daniel. USDA. ARS Poisonous Plant Research; Estados Unidos 2019-12-16T12:55:45Z 2019-12-16T12:55:45Z 2019-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6518 https://www.sciencedirect.com/science/article/pii/S0041010119304702 0041-0101 https://doi.org/10.1016/j.toxicon.2019.09.020 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Toxicon 171 (5) : 1-6 (Diciembre 2019) |
| spellingShingle | Astragalus Toxicidad Toxicidad por Ingestión Fitotoxicidad Envenenamiento Sanidad Animal Toxicity Ingestion Toxicity Phytotoxicity Poisoning Animal Health Swainsonine Región Patagónica Martinez, Agustin Robles, Carlos Alejandro Roper, Jessie M. Gardner, Dale R. Neyaz, Marwah S. Joelson, Natalia Zoe Cook, Daniel Detection of swainsonine-producing endophytes in Patagonian Astragalus species |
| title | Detection of swainsonine-producing endophytes in Patagonian Astragalus species |
| title_full | Detection of swainsonine-producing endophytes in Patagonian Astragalus species |
| title_fullStr | Detection of swainsonine-producing endophytes in Patagonian Astragalus species |
| title_full_unstemmed | Detection of swainsonine-producing endophytes in Patagonian Astragalus species |
| title_short | Detection of swainsonine-producing endophytes in Patagonian Astragalus species |
| title_sort | detection of swainsonine producing endophytes in patagonian astragalus species |
| topic | Astragalus Toxicidad Toxicidad por Ingestión Fitotoxicidad Envenenamiento Sanidad Animal Toxicity Ingestion Toxicity Phytotoxicity Poisoning Animal Health Swainsonine Región Patagónica |
| url | http://hdl.handle.net/20.500.12123/6518 https://www.sciencedirect.com/science/article/pii/S0041010119304702 https://doi.org/10.1016/j.toxicon.2019.09.020 |
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