Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput
Aims Characterization of barley-tissue–colonization efficiency (Hordeum vulgare L.) by Paraburkholderia tropica MTo-293 after seed inoculation was studied in two plant-growth systems: (1) in flasks with semisolid agar-containing sterile medium, as a suitable environment to study plant-bacteria inter...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer International Publishing
2019
|
| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007%2Fs11104-019-04174-y http://hdl.handle.net/20.500.12123/6502 https://doi.org/10.1007/s11104-019-04174-y |
| _version_ | 1855035688259420160 |
|---|---|
| author | García, Sabrina Soledad Bernabeu, Pamela Romina Vio, Santiago Adolfo Cattelan, Natalia Garcia, Julia Elena Puente, Mariana Laura María Lina, Galar Prieto, Claudia Inés Luna, María Flavia |
| author_browse | Bernabeu, Pamela Romina Cattelan, Natalia Garcia, Julia Elena García, Sabrina Soledad Luna, María Flavia María Lina, Galar Prieto, Claudia Inés Puente, Mariana Laura Vio, Santiago Adolfo |
| author_facet | García, Sabrina Soledad Bernabeu, Pamela Romina Vio, Santiago Adolfo Cattelan, Natalia Garcia, Julia Elena Puente, Mariana Laura María Lina, Galar Prieto, Claudia Inés Luna, María Flavia |
| author_sort | García, Sabrina Soledad |
| collection | INTA Digital |
| description | Aims Characterization of barley-tissue–colonization efficiency (Hordeum vulgare L.) by Paraburkholderia tropica MTo-293 after seed inoculation was studied in two plant-growth systems: (1) in flasks with semisolid agar-containing sterile medium, as a suitable environment to study plant-bacteria interaction and also optimize molecular-biology techniques, and (2) in a potsubstrate system as an approach to understand their potential behavior as bioinput. Methods Culture-dependent techniques were implemented to quantify surface and endophytic bacterial populations in plant tissues. Culture-independent techniques were employed to detect and localize the inoculated bacteria in tissue samples along with evaluating the biofilm-forming capability by epifluorescent, confocallaser-
scanning, and scanning-electron microscopy. Plant-growth parameters were measured to evaluate the effects of the inoculated acbteria on the development of the barley plants. Results Paraburkholderia tropica grew as a biofilm on both abiotic and biotic surfaces and efficiently colonized barley roots and stems in plants grown in flasks in presence of other microorganisms. The bacteria was localized on root surfaces, hairs, and central-cylinder areas. Paraburkholderia tropica also colonized the roots and stems in plants grown in the pot-substrate system. Although no endophytic root colonization occurred, the presence of the inoculated bacteria improved the aerial weight. A nested PCR detected P. tropica in the tissue samples. Conclusions Paraburkholderia tropica MTo-293 was characterized as an efficient biofilm-forming and barley- tissue–colonizing bacterium despite the presence of other microorganisms, but root endophytic colonization resulted dependent on the plant-growth system. Molecular-biology techniques were optimized, and also, its presence was correlated with plant-growth–promoting activity. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6502 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer International Publishing |
| publisherStr | Springer International Publishing |
| record_format | dspace |
| spelling | INTA65022019-12-12T18:39:03Z Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput García, Sabrina Soledad Bernabeu, Pamela Romina Vio, Santiago Adolfo Cattelan, Natalia Garcia, Julia Elena Puente, Mariana Laura María Lina, Galar Prieto, Claudia Inés Luna, María Flavia Barley Cebada Biofilm Bioinput Colonization Plant-growth–promoting bacteria Paraburkholderia Aims Characterization of barley-tissue–colonization efficiency (Hordeum vulgare L.) by Paraburkholderia tropica MTo-293 after seed inoculation was studied in two plant-growth systems: (1) in flasks with semisolid agar-containing sterile medium, as a suitable environment to study plant-bacteria interaction and also optimize molecular-biology techniques, and (2) in a potsubstrate system as an approach to understand their potential behavior as bioinput. Methods Culture-dependent techniques were implemented to quantify surface and endophytic bacterial populations in plant tissues. Culture-independent techniques were employed to detect and localize the inoculated bacteria in tissue samples along with evaluating the biofilm-forming capability by epifluorescent, confocallaser- scanning, and scanning-electron microscopy. Plant-growth parameters were measured to evaluate the effects of the inoculated acbteria on the development of the barley plants. Results Paraburkholderia tropica grew as a biofilm on both abiotic and biotic surfaces and efficiently colonized barley roots and stems in plants grown in flasks in presence of other microorganisms. The bacteria was localized on root surfaces, hairs, and central-cylinder areas. Paraburkholderia tropica also colonized the roots and stems in plants grown in the pot-substrate system. Although no endophytic root colonization occurred, the presence of the inoculated bacteria improved the aerial weight. A nested PCR detected P. tropica in the tissue samples. Conclusions Paraburkholderia tropica MTo-293 was characterized as an efficient biofilm-forming and barley- tissue–colonizing bacterium despite the presence of other microorganisms, but root endophytic colonization resulted dependent on the plant-growth system. Molecular-biology techniques were optimized, and also, its presence was correlated with plant-growth–promoting activity. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Garcia, Sabrina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Bernabeu, Pamela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Vio, Santiago Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: María Lina, Galar. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Prieto, Claudia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI); Argentina Fil: Luna, María Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico (CCT) La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI). Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA)La Plata; Argentina 2019-12-12T18:32:55Z 2019-12-12T18:32:55Z 2019-06-26 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007%2Fs11104-019-04174-y http://hdl.handle.net/20.500.12123/6502 0032-079X 1573-5036 https://doi.org/10.1007/s11104-019-04174-y eng info:eu-repo/semantics/restrictedAccess application/pdf Springer International Publishing Plant Soil (26 June 2019) |
| spellingShingle | Barley Cebada Biofilm Bioinput Colonization Plant-growth–promoting bacteria Paraburkholderia García, Sabrina Soledad Bernabeu, Pamela Romina Vio, Santiago Adolfo Cattelan, Natalia Garcia, Julia Elena Puente, Mariana Laura María Lina, Galar Prieto, Claudia Inés Luna, María Flavia Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput |
| title | Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput |
| title_full | Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput |
| title_fullStr | Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput |
| title_full_unstemmed | Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput |
| title_short | Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput |
| title_sort | paraburkholderia tropica as a plant growth promoting bacterium in barley characterization of tissues colonization by culture dependent and independent techniques for use as an agronomic bioinput |
| topic | Barley Cebada Biofilm Bioinput Colonization Plant-growth–promoting bacteria Paraburkholderia |
| url | https://link.springer.com/article/10.1007%2Fs11104-019-04174-y http://hdl.handle.net/20.500.12123/6502 https://doi.org/10.1007/s11104-019-04174-y |
| work_keys_str_mv | AT garciasabrinasoledad paraburkholderiatropicaasaplantgrowthpromotingbacteriuminbarleycharacterizationoftissuescolonizationbyculturedependentandindependenttechniquesforuseasanagronomicbioinput AT bernabeupamelaromina paraburkholderiatropicaasaplantgrowthpromotingbacteriuminbarleycharacterizationoftissuescolonizationbyculturedependentandindependenttechniquesforuseasanagronomicbioinput AT viosantiagoadolfo paraburkholderiatropicaasaplantgrowthpromotingbacteriuminbarleycharacterizationoftissuescolonizationbyculturedependentandindependenttechniquesforuseasanagronomicbioinput AT cattelannatalia paraburkholderiatropicaasaplantgrowthpromotingbacteriuminbarleycharacterizationoftissuescolonizationbyculturedependentandindependenttechniquesforuseasanagronomicbioinput AT garciajuliaelena paraburkholderiatropicaasaplantgrowthpromotingbacteriuminbarleycharacterizationoftissuescolonizationbyculturedependentandindependenttechniquesforuseasanagronomicbioinput AT puentemarianalaura paraburkholderiatropicaasaplantgrowthpromotingbacteriuminbarleycharacterizationoftissuescolonizationbyculturedependentandindependenttechniquesforuseasanagronomicbioinput AT marialinagalar paraburkholderiatropicaasaplantgrowthpromotingbacteriuminbarleycharacterizationoftissuescolonizationbyculturedependentandindependenttechniquesforuseasanagronomicbioinput AT prietoclaudiaines paraburkholderiatropicaasaplantgrowthpromotingbacteriuminbarleycharacterizationoftissuescolonizationbyculturedependentandindependenttechniquesforuseasanagronomicbioinput AT lunamariaflavia paraburkholderiatropicaasaplantgrowthpromotingbacteriuminbarleycharacterizationoftissuescolonizationbyculturedependentandindependenttechniquesforuseasanagronomicbioinput |