Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV)
Bovine leukemia virus (BLV) is a retrovirus that affects cattle causing a lymphoproliferative disease. BLV infection has been associated with misbalance of the immune response causing a higher incidence of other infections. Mastitis is one of the most important conditions that affect milk production...
| Main Authors: | , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2019
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| Subjects: | |
| Online Access: | https://www.sciencedirect.com/science/article/pii/S0168170218302508 http://hdl.handle.net/20.500.12123/6496 https://doi.org/10.1016/j.virusres.2018.07.013 |
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| author | Martinez Cuesta, Lucia Lendez, Pamela Anahi Barone, Lucas Perez, Sandra Elizabeth Dolcini, Guillermina Laura Ceriani, Maria Carolina Nieto Farias, Maria Victoria |
| author_browse | Barone, Lucas Ceriani, Maria Carolina Dolcini, Guillermina Laura Lendez, Pamela Anahi Martinez Cuesta, Lucia Nieto Farias, Maria Victoria Perez, Sandra Elizabeth |
| author_facet | Martinez Cuesta, Lucia Lendez, Pamela Anahi Barone, Lucas Perez, Sandra Elizabeth Dolcini, Guillermina Laura Ceriani, Maria Carolina Nieto Farias, Maria Victoria |
| author_sort | Martinez Cuesta, Lucia |
| collection | INTA Digital |
| description | Bovine leukemia virus (BLV) is a retrovirus that affects cattle causing a lymphoproliferative disease. BLV infection has been associated with misbalance of the immune response causing a higher incidence of other infections. Mastitis is one of the most important conditions that affect milk production in cattle. The aim of this study was to stably infect a bovine mammary epithelial cell line (MAC-T). MAC-T cell line was successfully infected with BLV and the infection was confirmed by nested PCR, qPCR, immunocytochemistry, western blot and transmission electron microscopy. This is the first report of a bovine mammary epithelial cell line stably infected with BLV. This new cell line could be used as an in vitro model to study the effect of BLV on the immune
response in the mammary gland and the relationship with other agents causing mastitis. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6496 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA64962019-12-12T14:53:55Z Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV) Martinez Cuesta, Lucia Lendez, Pamela Anahi Barone, Lucas Perez, Sandra Elizabeth Dolcini, Guillermina Laura Ceriani, Maria Carolina Nieto Farias, Maria Victoria Bovine Leukaemia Virus Infection Virus Leucemia Bovina Mmastitis infección MAC-T Bovine leukemia virus (BLV) is a retrovirus that affects cattle causing a lymphoproliferative disease. BLV infection has been associated with misbalance of the immune response causing a higher incidence of other infections. Mastitis is one of the most important conditions that affect milk production in cattle. The aim of this study was to stably infect a bovine mammary epithelial cell line (MAC-T). MAC-T cell line was successfully infected with BLV and the infection was confirmed by nested PCR, qPCR, immunocytochemistry, western blot and transmission electron microscopy. This is the first report of a bovine mammary epithelial cell line stably infected with BLV. This new cell line could be used as an in vitro model to study the effect of BLV on the immune response in the mammary gland and the relationship with other agents causing mastitis. Instituto de Virología Fil: Martinez Cuesta, Lucia. Consejo Nacional de Investigaciones Cientificas y Tecnicas (Conicet). Centro de Investigación Veterinaria de Tandil. Laboratorio de Virología. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Lendez, Pamela Anahi. Consejo Nacional de Investigaciones Cientificas y Tecnicas (Conicet). Centro de Investigación Veterinaria de Tandil. Laboratorio de Virología. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Barone, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Pérez, Sandra Elizabeth. Consejo Nacional de Investigaciones Cientificas y Tecnicas (Conicet). Centro de Investigación Veterinaria de Tandil. Laboratorio de Virología. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas (Conicet). Centro de Investigación Veterinaria de Tandil. Laboratorio de Virología. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas (Conicet). Centro de Investigación Veterinaria de Tandil. Laboratorio de Virología. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Nieto Farias, Maria Victoria. Consejo Nacional de Investigaciones Cientificas y Tecnicas (Conicet). Centro de Investigación Veterinaria de Tandil. Laboratorio de Virología. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina 2019-12-12T14:45:07Z 2019-12-12T14:45:07Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0168170218302508 http://hdl.handle.net/20.500.12123/6496 0168-1702 https://doi.org/10.1016/j.virusres.2018.07.013 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Volume 256 : 11-16 (September 2018) |
| spellingShingle | Bovine Leukaemia Virus Infection Virus Leucemia Bovina Mmastitis infección MAC-T Martinez Cuesta, Lucia Lendez, Pamela Anahi Barone, Lucas Perez, Sandra Elizabeth Dolcini, Guillermina Laura Ceriani, Maria Carolina Nieto Farias, Maria Victoria Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV) |
| title | Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV) |
| title_full | Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV) |
| title_fullStr | Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV) |
| title_full_unstemmed | Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV) |
| title_short | Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV) |
| title_sort | stable infection of a bovine mammary epithelial cell line mac t with bovine leukemia virus blv |
| topic | Bovine Leukaemia Virus Infection Virus Leucemia Bovina Mmastitis infección MAC-T |
| url | https://www.sciencedirect.com/science/article/pii/S0168170218302508 http://hdl.handle.net/20.500.12123/6496 https://doi.org/10.1016/j.virusres.2018.07.013 |
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