Stratification temperature affects the In Vitro germination of immature Prunus embryos
The rapid multiplication of clonal plants is desirable to shorten crossing programs in fruit tree breeding. In vitro stratification and germination allowed us to shorten the time and to improve the germination rates by embryo rescue. In this work, two stratification temperatures (0º and 4ºC) were co...
| Autores principales: | , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Español |
| Publicado: |
ISHS
2019
|
| Materias: | |
| Acceso en línea: | https://www.actahort.org/books/658/658_18.htm http://hdl.handle.net/20.500.12123/6490 https://doi.org/10.17660/ActaHortic.2004.658.18 |
| _version_ | 1855483823185199104 |
|---|---|
| author | Daorden, Maria Elena Marin, Juan Antonio Arbeloa, Arancha |
| author_browse | Arbeloa, Arancha Daorden, Maria Elena Marin, Juan Antonio |
| author_facet | Daorden, Maria Elena Marin, Juan Antonio Arbeloa, Arancha |
| author_sort | Daorden, Maria Elena |
| collection | INTA Digital |
| description | The rapid multiplication of clonal plants is desirable to shorten crossing programs in fruit tree breeding. In vitro stratification and germination allowed us to shorten the time and to improve the germination rates by embryo rescue. In this work, two stratification temperatures (0º and 4ºC) were compared and related to the development of interspecific hybrid embryos. The influence of embryo size was also determined, in order to optimise the process. Data showed that germination took place in 91% of the seeds when the stratification temperature was 4ºC. During stratification, embryo weight and embryo-weight increment were assessed at 30 and 60 days after sowing. Stratification temperature showed a significant effect in both embryo weight and embryo-weight increment 60 days after sowing, while embryo size showed a smaller influence. Embryo culture and stratification at 4 ºC allowed a satisfactory germination of immature seeds produced in crosses from breeding programs. |
| format | Artículo |
| id | INTA6490 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Español |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | ISHS |
| publisherStr | ISHS |
| record_format | dspace |
| spelling | INTA64902019-12-12T11:21:14Z Stratification temperature affects the In Vitro germination of immature Prunus embryos Daorden, Maria Elena Marin, Juan Antonio Arbeloa, Arancha Prunus Cultivo de Embriones Embriones Vegetales Germinación Semillas Frutales Fitomejoramiento Embryo Culture Plant Embryos Germination Seeds Fruit Crops Plant Breeding Estratificación in Vitro Híbridos Interespecíficos In vitro Stratification Interspecific Hybrids The rapid multiplication of clonal plants is desirable to shorten crossing programs in fruit tree breeding. In vitro stratification and germination allowed us to shorten the time and to improve the germination rates by embryo rescue. In this work, two stratification temperatures (0º and 4ºC) were compared and related to the development of interspecific hybrid embryos. The influence of embryo size was also determined, in order to optimise the process. Data showed that germination took place in 91% of the seeds when the stratification temperature was 4ºC. During stratification, embryo weight and embryo-weight increment were assessed at 30 and 60 days after sowing. Stratification temperature showed a significant effect in both embryo weight and embryo-weight increment 60 days after sowing, while embryo size showed a smaller influence. Embryo culture and stratification at 4 ºC allowed a satisfactory germination of immature seeds produced in crosses from breeding programs. EEA San Pedro Fil: Daorden, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Marin, Juan Antonio. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental de Aula Dei. Zaragoza; España Fil: Arbeloa, Arancha. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental de Aula Dei. Zaragoza; España 2019-12-12T11:11:37Z 2019-12-12T11:11:37Z 2004 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.actahort.org/books/658/658_18.htm http://hdl.handle.net/20.500.12123/6490 978-90-66051-90-4 0567-7572 2406-6168 https://doi.org/10.17660/ActaHortic.2004.658.18 spa info:eu-repo/semantics/restrictedAccess application/pdf ISHS Acta horticulturae 658 :135-140 (Oct. 2004) |
| spellingShingle | Prunus Cultivo de Embriones Embriones Vegetales Germinación Semillas Frutales Fitomejoramiento Embryo Culture Plant Embryos Germination Seeds Fruit Crops Plant Breeding Estratificación in Vitro Híbridos Interespecíficos In vitro Stratification Interspecific Hybrids Daorden, Maria Elena Marin, Juan Antonio Arbeloa, Arancha Stratification temperature affects the In Vitro germination of immature Prunus embryos |
| title | Stratification temperature affects the In Vitro germination of immature Prunus embryos |
| title_full | Stratification temperature affects the In Vitro germination of immature Prunus embryos |
| title_fullStr | Stratification temperature affects the In Vitro germination of immature Prunus embryos |
| title_full_unstemmed | Stratification temperature affects the In Vitro germination of immature Prunus embryos |
| title_short | Stratification temperature affects the In Vitro germination of immature Prunus embryos |
| title_sort | stratification temperature affects the in vitro germination of immature prunus embryos |
| topic | Prunus Cultivo de Embriones Embriones Vegetales Germinación Semillas Frutales Fitomejoramiento Embryo Culture Plant Embryos Germination Seeds Fruit Crops Plant Breeding Estratificación in Vitro Híbridos Interespecíficos In vitro Stratification Interspecific Hybrids |
| url | https://www.actahort.org/books/658/658_18.htm http://hdl.handle.net/20.500.12123/6490 https://doi.org/10.17660/ActaHortic.2004.658.18 |
| work_keys_str_mv | AT daordenmariaelena stratificationtemperatureaffectstheinvitrogerminationofimmatureprunusembryos AT marinjuanantonio stratificationtemperatureaffectstheinvitrogerminationofimmatureprunusembryos AT arbeloaarancha stratificationtemperatureaffectstheinvitrogerminationofimmatureprunusembryos |