Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels
Background and Aims: Experimental evidence in the literature suggests that O2•− produced in the elongation zone of roots and leaves by plasma membrane NADPH oxidase activity is required for growth. This study explores whether growth changes along the root tip induced by hyperosmotic treatments in Ze...
| Main Authors: | , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Oxford Academic Press
2019
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/6474 https://academic.oup.com/aob/article/102/4/551/166437 https://doi.org/10.1093/aob/mcn141 |
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| author | Bustos, Dolores Angela Lascano, Hernan Ramiro Villasuso, Ana Laura Machado, Estela Senn, Eugenia Cordoba, Alicia Beatriz Taleisnik, Edith |
| author_browse | Bustos, Dolores Angela Cordoba, Alicia Beatriz Lascano, Hernan Ramiro Machado, Estela Senn, Eugenia Taleisnik, Edith Villasuso, Ana Laura |
| author_facet | Bustos, Dolores Angela Lascano, Hernan Ramiro Villasuso, Ana Laura Machado, Estela Senn, Eugenia Cordoba, Alicia Beatriz Taleisnik, Edith |
| author_sort | Bustos, Dolores Angela |
| collection | INTA Digital |
| description | Background and Aims: Experimental evidence in the literature suggests that O2•− produced in the elongation zone of roots and leaves by plasma membrane NADPH oxidase activity is required for growth. This study explores whether growth changes along the root tip induced by hyperosmotic treatments in Zea mays are associated with the distribution of apoplastic O2•−.
Methods: Stress treatments were imposed using 150 mM NaCl or 300 mM sorbitol. Root elongation rates and the spatial distribution of growth rates in the root tip were measured. Apoplastic O2•− was determined using nitro blue tetrazolium, and H2O2 was determined using 2′, 7′-dichlorofluorescin.
Key Results: In non-stressed plants, the distribution of accelerating growth and highest O2•− levels coincided along the root tip. Salt and osmotic stress of the same intensity had similar inhibitory effects on root elongation, but O2•− levels increased in sorbitol-treated roots and decreased in NaCl-treated roots.
Conclusions: The lack of association between apoplastic O2•− levels and root growth inhibition under hyper-osmotic stress leads us to hypothesize that under those conditions the role of apoplastic O2•− may be to participate in signalling processes, that convey information on the nature of the substrate that the growing root is exploring. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6474 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford Academic Press |
| publisherStr | Oxford Academic Press |
| record_format | dspace |
| spelling | INTA64742022-10-13T11:18:49Z Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels Bustos, Dolores Angela Lascano, Hernan Ramiro Villasuso, Ana Laura Machado, Estela Senn, Eugenia Cordoba, Alicia Beatriz Taleisnik, Edith Maíz Zea Mays Estrés Osmótico Enraizamiento Especies de Oxígeno Reactivo Maize Osmotic Stress Rooting Reactive Oxygen Species Estres Salino Background and Aims: Experimental evidence in the literature suggests that O2•− produced in the elongation zone of roots and leaves by plasma membrane NADPH oxidase activity is required for growth. This study explores whether growth changes along the root tip induced by hyperosmotic treatments in Zea mays are associated with the distribution of apoplastic O2•−. Methods: Stress treatments were imposed using 150 mM NaCl or 300 mM sorbitol. Root elongation rates and the spatial distribution of growth rates in the root tip were measured. Apoplastic O2•− was determined using nitro blue tetrazolium, and H2O2 was determined using 2′, 7′-dichlorofluorescin. Key Results: In non-stressed plants, the distribution of accelerating growth and highest O2•− levels coincided along the root tip. Salt and osmotic stress of the same intensity had similar inhibitory effects on root elongation, but O2•− levels increased in sorbitol-treated roots and decreased in NaCl-treated roots. Conclusions: The lack of association between apoplastic O2•− levels and root growth inhibition under hyper-osmotic stress leads us to hypothesize that under those conditions the role of apoplastic O2•− may be to participate in signalling processes, that convey information on the nature of the substrate that the growing root is exploring. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Fil: Villasuso, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físicas, Químicas y Naturales. Química Biológica; Argentina Fil: Machado, Estela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físicas, Químicas y Naturales. Química Biológica; Argentina Fil: Senn, Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Fil: Cordoba, Alicia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Fil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. 2019-12-09T14:30:58Z 2019-12-09T14:30:58Z 2008-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6474 https://academic.oup.com/aob/article/102/4/551/166437 0305-7364 1095-8290 https://doi.org/10.1093/aob/mcn141 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Oxford Academic Press Annals of Botany 102 (4) : 551–559 (October 2008) |
| spellingShingle | Maíz Zea Mays Estrés Osmótico Enraizamiento Especies de Oxígeno Reactivo Maize Osmotic Stress Rooting Reactive Oxygen Species Estres Salino Bustos, Dolores Angela Lascano, Hernan Ramiro Villasuso, Ana Laura Machado, Estela Senn, Eugenia Cordoba, Alicia Beatriz Taleisnik, Edith Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels |
| title | Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels |
| title_full | Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels |
| title_fullStr | Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels |
| title_full_unstemmed | Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels |
| title_short | Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels |
| title_sort | reductions in maize root tip elongation by salt and osmotic stress do not correlate with apoplastic o2• levels |
| topic | Maíz Zea Mays Estrés Osmótico Enraizamiento Especies de Oxígeno Reactivo Maize Osmotic Stress Rooting Reactive Oxygen Species Estres Salino |
| url | http://hdl.handle.net/20.500.12123/6474 https://academic.oup.com/aob/article/102/4/551/166437 https://doi.org/10.1093/aob/mcn141 |
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