Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characte...
| Main Authors: | , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Oxford Academic Press
2019
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| Online Access: | http://hdl.handle.net/20.500.12123/6472 https://academic.oup.com/ps/article/96/8/2820/3096894 https://doi.org/10.3382/ps/pex053 |
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| author | Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Godano, Eduardo Ignacio Gómez, S.C. ViaButron, I.A. Padin, V.M. Rogé, Ariel Diego |
| author_browse | Bueno, Dante Javier Godano, Eduardo Ignacio Gómez, S.C. Padin, V.M. Rogé, Ariel Diego Soria, Maria Cecilia Soria, Mario ViaButron, I.A. |
| author_facet | Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Godano, Eduardo Ignacio Gómez, S.C. ViaButron, I.A. Padin, V.M. Rogé, Ariel Diego |
| author_sort | Soria, Maria Cecilia |
| collection | INTA Digital |
| description | The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6472 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford Academic Press |
| publisherStr | Oxford Academic Press |
| record_format | dspace |
| spelling | INTA64722023-09-18T13:13:42Z Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Godano, Eduardo Ignacio Gómez, S.C. ViaButron, I.A. Padin, V.M. Rogé, Ariel Diego Gallina Ponedora Enfermedades de los Animales Salmonella Técnicas de Cultivo PCR Layer Chickens Animal Diseases Culture Techniques The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence. EEA Concepción del Uruguay Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Godano, E.I. Tecnovo S.A.; Argentina Fil: Gómez, S.C. Fundación ArgenINTA, Paraná; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Concepción del Uruguay; Argentina Fil: ViaButron, I.A.. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Fil: Padin, V.M. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; Argentina Fil: Rogé, Ariel D. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; Argentina 2019-12-09T12:24:49Z 2019-12-09T12:24:49Z 2017-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6472 https://academic.oup.com/ps/article/96/8/2820/3096894 0032-5791 1525-3171 https://doi.org/10.3382/ps/pex053 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Oxford Academic Press Poultry Science 96 (8) : 2820–2830 (August 2017) |
| spellingShingle | Gallina Ponedora Enfermedades de los Animales Salmonella Técnicas de Cultivo PCR Layer Chickens Animal Diseases Culture Techniques Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Godano, Eduardo Ignacio Gómez, S.C. ViaButron, I.A. Padin, V.M. Rogé, Ariel Diego Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
| title | Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
| title_full | Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
| title_fullStr | Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
| title_full_unstemmed | Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
| title_short | Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
| title_sort | salmonella spp contamination in commercial layer hen farms using different types of samples and detection methods |
| topic | Gallina Ponedora Enfermedades de los Animales Salmonella Técnicas de Cultivo PCR Layer Chickens Animal Diseases Culture Techniques |
| url | http://hdl.handle.net/20.500.12123/6472 https://academic.oup.com/ps/article/96/8/2820/3096894 https://doi.org/10.3382/ps/pex053 |
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