A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water
The present work compared 2 culture methods and PCR assays for motile and nonmotile Salmonella detection using artificially contaminated poultry drinking water. The specificity was 1 for all methods studied. The accuracy and sensitivity were 1 for all motile strains, whereas these parameters were be...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Oxford Academic Press
2019
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| Acceso en línea: | https://academic.oup.com/ps/article/92/1/225/1554522 http://hdl.handle.net/20.500.12123/6468 https://doi.org/10.3382/ps.2012-02254 |
| _version_ | 1855483817734701056 |
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| author | Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier |
| author_browse | Bueno, Dante Javier Soria, Maria Cecilia Soria, Mario |
| author_facet | Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier |
| author_sort | Soria, Maria Cecilia |
| collection | INTA Digital |
| description | The present work compared 2 culture methods and PCR assays for motile and nonmotile Salmonella detection using artificially contaminated poultry drinking water. The specificity was 1 for all methods studied. The accuracy and sensitivity were 1 for all motile strains, whereas these parameters were between 0 and 0.7 for nonmotile Salmonella strains. The positive predictive value and negative predictive value were 1 for all motile Salmonella strains in the 3 methods used. Nonmotile Salmonella strains showed a positive predictive value of 1 in the PCR method. However, the positive predictive value was indeterminate in the tetrathionate (TT) methods for both strains tested and in the modified semisolid Rappaport-Vassiliadis (MSRV) method for Salmonella Pullorum. On the other hand, the negative predictive value was between 0.20 and 0.43 for the 3 methods. The detection level of motile strains was 4 to 7 cfu/25 mL for all methods. Nonmotile Salmonella strains could not be detected in the TT method, whereas only Salmonella Gallinarum could be recovered from 1.1 × 101 cfu/25 mL in the MSRV method. In relation to the molecular methods, PCR could detect these strains from 1.1 × 104 cfu/25 mL. Extending incubation time of the enrichment medium to 6 d in the TT method did not improve the isolation rates. In general, all selective plating media did not show any statistical differences in the parameters of performance studied. The kappa coefficient showed that there was an excellent agreement between the 3 methods for motile strains. For nonmotile strains, the agreement was poor between the MSRV and the PCR; there was no agreement when the TT method was compared with the MSRV and the PCR methods. The difference in detection levels obtained with the methods used for motile and nonmotile Salmonella strains and the difficulty in detecting these last strains represents a potential problem when a poultry water sample is considered negative for the presence of Salmonella. |
| format | Artículo |
| id | INTA6468 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford Academic Press |
| publisherStr | Oxford Academic Press |
| record_format | dspace |
| spelling | INTA64682019-12-06T14:52:54Z A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Aves de Corral Salmonella Agua Potable Enfermedades de los Animales Técnicas de Cultivo PCR Poultry Drinking Water Animal Diseases Culture Techniques The present work compared 2 culture methods and PCR assays for motile and nonmotile Salmonella detection using artificially contaminated poultry drinking water. The specificity was 1 for all methods studied. The accuracy and sensitivity were 1 for all motile strains, whereas these parameters were between 0 and 0.7 for nonmotile Salmonella strains. The positive predictive value and negative predictive value were 1 for all motile Salmonella strains in the 3 methods used. Nonmotile Salmonella strains showed a positive predictive value of 1 in the PCR method. However, the positive predictive value was indeterminate in the tetrathionate (TT) methods for both strains tested and in the modified semisolid Rappaport-Vassiliadis (MSRV) method for Salmonella Pullorum. On the other hand, the negative predictive value was between 0.20 and 0.43 for the 3 methods. The detection level of motile strains was 4 to 7 cfu/25 mL for all methods. Nonmotile Salmonella strains could not be detected in the TT method, whereas only Salmonella Gallinarum could be recovered from 1.1 × 101 cfu/25 mL in the MSRV method. In relation to the molecular methods, PCR could detect these strains from 1.1 × 104 cfu/25 mL. Extending incubation time of the enrichment medium to 6 d in the TT method did not improve the isolation rates. In general, all selective plating media did not show any statistical differences in the parameters of performance studied. The kappa coefficient showed that there was an excellent agreement between the 3 methods for motile strains. For nonmotile strains, the agreement was poor between the MSRV and the PCR; there was no agreement when the TT method was compared with the MSRV and the PCR methods. The difference in detection levels obtained with the methods used for motile and nonmotile Salmonella strains and the difficulty in detecting these last strains represents a potential problem when a poultry water sample is considered negative for the presence of Salmonella. EEA Concepción del Uruguay Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina 2019-12-06T14:51:32Z 2019-12-06T14:51:32Z 2013-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/ps/article/92/1/225/1554522 http://hdl.handle.net/20.500.12123/6468 0032-5791 1525-3171 https://doi.org/10.3382/ps.2012-02254 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Oxford Academic Press Poultry Science 92 (1) : 225–232 (January 2013) |
| spellingShingle | Aves de Corral Salmonella Agua Potable Enfermedades de los Animales Técnicas de Cultivo PCR Poultry Drinking Water Animal Diseases Culture Techniques Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water |
| title | A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water |
| title_full | A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water |
| title_fullStr | A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water |
| title_full_unstemmed | A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water |
| title_short | A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water |
| title_sort | comparative study of culture methods and pcr assay for salmonella detection in poultry drinking water |
| topic | Aves de Corral Salmonella Agua Potable Enfermedades de los Animales Técnicas de Cultivo PCR Poultry Drinking Water Animal Diseases Culture Techniques |
| url | https://academic.oup.com/ps/article/92/1/225/1554522 http://hdl.handle.net/20.500.12123/6468 https://doi.org/10.3382/ps.2012-02254 |
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