A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content
The present work compared 2 culture methods and a PCR assay applied with 2 enrichment methods for the detection of motile and nonmotile Salmonella strains using artificially contaminated egg content. The specificity (Sp) was 1 in all methods. The sensitivity (Se), accuracy (Ac), positive predictive...
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| Format: | Artículo |
| Language: | Inglés |
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Oxford Academic Press
2019
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| Online Access: | https://academic.oup.com/ps/article/91/10/2668/1561306 http://hdl.handle.net/20.500.12123/6467 https://doi.org/10.3382/ps.2012-02253 |
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| author | Soria, Mario Soria, Maria Cecilia Bueno, Dante Javier |
| author_browse | Bueno, Dante Javier Soria, Maria Cecilia Soria, Mario |
| author_facet | Soria, Mario Soria, Maria Cecilia Bueno, Dante Javier |
| author_sort | Soria, Mario |
| collection | INTA Digital |
| description | The present work compared 2 culture methods and a PCR assay applied with 2 enrichment methods for the detection of motile and nonmotile Salmonella strains using artificially contaminated egg content. The specificity (Sp) was 1 in all methods. The sensitivity (Se), accuracy (Ac), positive predictive value (PPV), and negative predictive value (NPV) were 1 in both culture methods for motile and nonmotile strains. In reference to the PCR methods, Se and PPV were between 0 and 1, whereas Ac and NPV were between 0.14 and 1. The detection level of motile and nonmotile strains was 5 to 54 cfu per 25 mL for both culture methods, but some strains could not be detected by the PCR methods. Extending incubation time of the enrichment medium to 5 d in the tetrathionate broth (TT), and Muller-Kauffmann tetrathionate-novobiocin broth (MKTTn) methods did not improve the isolation rates. All selective plating media did not show any statistical differences in the parameters of performance studied. Kappa coefficients showed that there was an excellent agreement between the bacteriological methods for all Salmonella strains. The agreement was very good and good between the PCR methods, for motile and nonmotile strains, respectively. However, there was a poor agreement when the PCR and bacteriological methods were compared for motile and nonmotile Salmonella strains. The TT and MKTTn methods are similar in terms of Ac, Se, Sp, PPV, and NPV for different Salmonella strains in egg content. The use of the PCR method cannot improve the same parameters, described before, in this matrix. So, further studies are needed to improve the performance parameters and limit of detection in egg content for the PCR methods, so that test can be used in poultry and food industry. |
| format | Artículo |
| id | INTA6467 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford Academic Press |
| publisherStr | Oxford Academic Press |
| record_format | dspace |
| spelling | INTA64672019-12-06T14:35:14Z A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content Soria, Mario Soria, Maria Cecilia Bueno, Dante Javier Aves de Corral Enfermedades de los Animales Salmonella PCR Técnicas de Cultivo Huevos Poultry Animal Diseases Culture Techniques Eggs The present work compared 2 culture methods and a PCR assay applied with 2 enrichment methods for the detection of motile and nonmotile Salmonella strains using artificially contaminated egg content. The specificity (Sp) was 1 in all methods. The sensitivity (Se), accuracy (Ac), positive predictive value (PPV), and negative predictive value (NPV) were 1 in both culture methods for motile and nonmotile strains. In reference to the PCR methods, Se and PPV were between 0 and 1, whereas Ac and NPV were between 0.14 and 1. The detection level of motile and nonmotile strains was 5 to 54 cfu per 25 mL for both culture methods, but some strains could not be detected by the PCR methods. Extending incubation time of the enrichment medium to 5 d in the tetrathionate broth (TT), and Muller-Kauffmann tetrathionate-novobiocin broth (MKTTn) methods did not improve the isolation rates. All selective plating media did not show any statistical differences in the parameters of performance studied. Kappa coefficients showed that there was an excellent agreement between the bacteriological methods for all Salmonella strains. The agreement was very good and good between the PCR methods, for motile and nonmotile strains, respectively. However, there was a poor agreement when the PCR and bacteriological methods were compared for motile and nonmotile Salmonella strains. The TT and MKTTn methods are similar in terms of Ac, Se, Sp, PPV, and NPV for different Salmonella strains in egg content. The use of the PCR method cannot improve the same parameters, described before, in this matrix. So, further studies are needed to improve the performance parameters and limit of detection in egg content for the PCR methods, so that test can be used in poultry and food industry. EEA Concepción del Uruguay Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina 2019-12-06T14:32:44Z 2019-12-06T14:32:44Z 2012-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/ps/article/91/10/2668/1561306 http://hdl.handle.net/20.500.12123/6467 0032-5791 1525-3171 https://doi.org/10.3382/ps.2012-02253 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Oxford Academic Press Poultry Science 91 (10) : 2668–2676 (October 2012) |
| spellingShingle | Aves de Corral Enfermedades de los Animales Salmonella PCR Técnicas de Cultivo Huevos Poultry Animal Diseases Culture Techniques Eggs Soria, Mario Soria, Maria Cecilia Bueno, Dante Javier A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
| title | A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
| title_full | A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
| title_fullStr | A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
| title_full_unstemmed | A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
| title_short | A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
| title_sort | comparative study of culture methods and polymerase chain reaction for salmonella detection in egg content |
| topic | Aves de Corral Enfermedades de los Animales Salmonella PCR Técnicas de Cultivo Huevos Poultry Animal Diseases Culture Techniques Eggs |
| url | https://academic.oup.com/ps/article/91/10/2668/1561306 http://hdl.handle.net/20.500.12123/6467 https://doi.org/10.3382/ps.2012-02253 |
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