A comparative study of culture methods and polymerase chain reaction assay for Salmonella detection in poultry feed
The present work compared 2 culture methods and PCR assay for the detection of motile and non-motile Salmonella strains using artificially contaminated poultry feed. The specificity was 1 in all methods. The accuracy and sensitivity were between 0.5 and 1 for motile Salmonella strains, whereas these...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Oxford Academic Press
2019
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| Acceso en línea: | https://academic.oup.com/ps/article/90/11/2606/1503580 http://hdl.handle.net/20.500.12123/6465 https://doi.org/10.3382/ps.2011-01548 |
| _version_ | 1855483816919957504 |
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| author | Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Colazo, Jose |
| author_browse | Bueno, Dante Javier Colazo, Jose Soria, Maria Cecilia Soria, Mario |
| author_facet | Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Colazo, Jose |
| author_sort | Soria, Maria Cecilia |
| collection | INTA Digital |
| description | The present work compared 2 culture methods and PCR assay for the detection of motile and non-motile Salmonella strains using artificially contaminated poultry feed. The specificity was 1 in all methods. The accuracy and sensitivity were between 0.5 and 1 for motile Salmonella strains, whereas these parameters were between 0 and 0.6 for non-motile Salmonella strains. The positive predictive value was 1 for tetrathionate (TT), PCR, and modified semisolid Rappaport-Vassiliadis (MSRV) methods in most of the strains studied. The negative predictive value of each method was very low for non-motile Salmonella strains. The detection level of motile strains was 8 to 20 cfu/25 g for all methods, whereas it was ≥104 cfu/25 g in culture methods for non-motile Salmonella strains. In general, the PCR method detected lower non-motile Salmonella contamination levels in feed than did culture methods. Extending incubation time of the enrichment medium to 6 d in the TT method did not improve the isolation rates. All selective plating media did not show any statistical differences in the parameters of performance studied. Kappa coefficients showed that there was good agreement between TT and MSRV methods, and MSRV and PCR methods for motile Salmonella strains in poultry feed samples. The agreement was fair between TT and PCR methods for these strains. For non-motile Salmonella strains, there was poor (TT and MSRV methods), slight (PCR and TT methods), and fair (MSRV and PCR methods) agreement. The TT, MSRV, and PCR methods are similar in terms of accuracy, sensitivity, specificity, positive predictive value, and negative predictive value for different motile Salmonella strains in poultry feed. For non-motile Salmonella strains, the use of the PCR method improves the same parameters, described before, in this matrix. The difference in detection levels obtained with the methods used for motile and nonmotile Salmonella strains and the difficulty to detect these last strains represent a potential problem, when a poultry feed sample is considered negative for the presence of Salmonella. |
| format | Artículo |
| id | INTA6465 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford Academic Press |
| publisherStr | Oxford Academic Press |
| record_format | dspace |
| spelling | INTA64652019-12-06T13:56:44Z A comparative study of culture methods and polymerase chain reaction assay for Salmonella detection in poultry feed Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Colazo, Jose Aves de Corral Enfermedades de los Animales Salmonella Técnicas de Cultivo PCR Alimentación Avícola Poultry Animal Diseases Culture Techniques Poultry Feeding The present work compared 2 culture methods and PCR assay for the detection of motile and non-motile Salmonella strains using artificially contaminated poultry feed. The specificity was 1 in all methods. The accuracy and sensitivity were between 0.5 and 1 for motile Salmonella strains, whereas these parameters were between 0 and 0.6 for non-motile Salmonella strains. The positive predictive value was 1 for tetrathionate (TT), PCR, and modified semisolid Rappaport-Vassiliadis (MSRV) methods in most of the strains studied. The negative predictive value of each method was very low for non-motile Salmonella strains. The detection level of motile strains was 8 to 20 cfu/25 g for all methods, whereas it was ≥104 cfu/25 g in culture methods for non-motile Salmonella strains. In general, the PCR method detected lower non-motile Salmonella contamination levels in feed than did culture methods. Extending incubation time of the enrichment medium to 6 d in the TT method did not improve the isolation rates. All selective plating media did not show any statistical differences in the parameters of performance studied. Kappa coefficients showed that there was good agreement between TT and MSRV methods, and MSRV and PCR methods for motile Salmonella strains in poultry feed samples. The agreement was fair between TT and PCR methods for these strains. For non-motile Salmonella strains, there was poor (TT and MSRV methods), slight (PCR and TT methods), and fair (MSRV and PCR methods) agreement. The TT, MSRV, and PCR methods are similar in terms of accuracy, sensitivity, specificity, positive predictive value, and negative predictive value for different motile Salmonella strains in poultry feed. For non-motile Salmonella strains, the use of the PCR method improves the same parameters, described before, in this matrix. The difference in detection levels obtained with the methods used for motile and nonmotile Salmonella strains and the difficulty to detect these last strains represent a potential problem, when a poultry feed sample is considered negative for the presence of Salmonella. EEA Concepción del Uruguay Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Colazo, Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina 2019-12-06T13:55:18Z 2019-12-06T13:55:18Z 2011-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/ps/article/90/11/2606/1503580 http://hdl.handle.net/20.500.12123/6465 0032-5791 1525-3171 https://doi.org/10.3382/ps.2011-01548 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Oxford Academic Press Poultry Science 90 (11) : 2606–2618 (November 2011) |
| spellingShingle | Aves de Corral Enfermedades de los Animales Salmonella Técnicas de Cultivo PCR Alimentación Avícola Poultry Animal Diseases Culture Techniques Poultry Feeding Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Colazo, Jose A comparative study of culture methods and polymerase chain reaction assay for Salmonella detection in poultry feed |
| title | A comparative study of culture methods and polymerase chain reaction assay for Salmonella detection in poultry feed |
| title_full | A comparative study of culture methods and polymerase chain reaction assay for Salmonella detection in poultry feed |
| title_fullStr | A comparative study of culture methods and polymerase chain reaction assay for Salmonella detection in poultry feed |
| title_full_unstemmed | A comparative study of culture methods and polymerase chain reaction assay for Salmonella detection in poultry feed |
| title_short | A comparative study of culture methods and polymerase chain reaction assay for Salmonella detection in poultry feed |
| title_sort | comparative study of culture methods and polymerase chain reaction assay for salmonella detection in poultry feed |
| topic | Aves de Corral Enfermedades de los Animales Salmonella Técnicas de Cultivo PCR Alimentación Avícola Poultry Animal Diseases Culture Techniques Poultry Feeding |
| url | https://academic.oup.com/ps/article/90/11/2606/1503580 http://hdl.handle.net/20.500.12123/6465 https://doi.org/10.3382/ps.2011-01548 |
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