Comparison of 7 culture methods for Salmonella serovar Enteritidis and Salmonella serovar Typhimurium isolation in poultry feces
The present work compared 7 different culture methods and 3 selective-differential plating media for Salmonella ser. Enteritidis (SE) and S. ser. Typhimurium (ST) isolation using artificially contaminated poultry feces. The sensitivity (Se) and accuracy (AC) values increased when Modified Semisolid...
| Main Authors: | , , |
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| Format: | Artículo |
| Language: | Inglés |
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Oxford Academic Press
2019
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/6462 https://doi.org/10.3382/ps/pey259 |
| _version_ | 1855483816134574080 |
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| author | Rodriguez, Francisco Procura, Francisco Bueno, Dante Javier |
| author_browse | Bueno, Dante Javier Procura, Francisco Rodriguez, Francisco |
| author_facet | Rodriguez, Francisco Procura, Francisco Bueno, Dante Javier |
| author_sort | Rodriguez, Francisco |
| collection | INTA Digital |
| description | The present work compared 7 different culture methods and 3 selective-differential plating media for Salmonella ser. Enteritidis (SE) and S. ser. Typhimurium (ST) isolation using artificially contaminated poultry feces. The sensitivity (Se) and accuracy (AC) values increased when Modified Semisolid Rappaport Vassiliadis (MSRV) methods were used in place of the Tetrathionate (TT) or Tetrathionate Hajna broth (TTH) method in the enrichment step. However, there was no significant difference between the pre-enrichment incubation at 4 to 6 and 18 to 24 h for MSRV5 and MSRV24 methods, respectively. All Salmonella strains were recovered in the lowest dilutions tested for MSRV24 and 3 out of 4 for MSRV5 methods (2 to 10 cfu/25 g). The TT and TTH methods showed a detection limit between 2.2 × 101 and 1.0 × 106 cfu/25 g of fecal sample. The agreement was variable between the methods. However, there was a very good agreement between the MSRV5 and MSRV24 methods, and between tetrathionate direct (TTD, no pre-enrichment media used) and buffered peptone water 18 to 24 h Tetrathionate broth combination (TT24 method) for Salmonella strains. The 3 selective-differential plating media showed an agreement between fair and excellent. They performed a high Se and AC in the MSRV methods for Salmonella strains. There was a significant difference between center and periphery for MSRV methods, and there was a fair agreement between them for all strains. The MSRV methods are better than TT/TTH methods for the isolation of different strains of SE and ST in poultry fecal samples. The MSRV5 method can be used to reduce the time for the detection of SE and ST in these samples. Furthermore, a loopful of the periphery of the growth should be streaked onto differential-selective plating media, even in the absence of halo, to decrease the number of false negative results. |
| format | Artículo |
| id | INTA6462 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford Academic Press |
| publisherStr | Oxford Academic Press |
| record_format | dspace |
| spelling | INTA64622022-10-31T13:13:37Z Comparison of 7 culture methods for Salmonella serovar Enteritidis and Salmonella serovar Typhimurium isolation in poultry feces Rodriguez, Francisco Procura, Francisco Bueno, Dante Javier Enfermedades de los Animales Salmonella Serotipos Salmonella enteritidis Aves de Corral Heces Técnicas de Cultivo Animal Diseases Serotypes Poultry Faeces Culture Techniques The present work compared 7 different culture methods and 3 selective-differential plating media for Salmonella ser. Enteritidis (SE) and S. ser. Typhimurium (ST) isolation using artificially contaminated poultry feces. The sensitivity (Se) and accuracy (AC) values increased when Modified Semisolid Rappaport Vassiliadis (MSRV) methods were used in place of the Tetrathionate (TT) or Tetrathionate Hajna broth (TTH) method in the enrichment step. However, there was no significant difference between the pre-enrichment incubation at 4 to 6 and 18 to 24 h for MSRV5 and MSRV24 methods, respectively. All Salmonella strains were recovered in the lowest dilutions tested for MSRV24 and 3 out of 4 for MSRV5 methods (2 to 10 cfu/25 g). The TT and TTH methods showed a detection limit between 2.2 × 101 and 1.0 × 106 cfu/25 g of fecal sample. The agreement was variable between the methods. However, there was a very good agreement between the MSRV5 and MSRV24 methods, and between tetrathionate direct (TTD, no pre-enrichment media used) and buffered peptone water 18 to 24 h Tetrathionate broth combination (TT24 method) for Salmonella strains. The 3 selective-differential plating media showed an agreement between fair and excellent. They performed a high Se and AC in the MSRV methods for Salmonella strains. There was a significant difference between center and periphery for MSRV methods, and there was a fair agreement between them for all strains. The MSRV methods are better than TT/TTH methods for the isolation of different strains of SE and ST in poultry fecal samples. The MSRV5 method can be used to reduce the time for the detection of SE and ST in these samples. Furthermore, a loopful of the periphery of the growth should be streaked onto differential-selective plating media, even in the absence of halo, to decrease the number of false negative results. EEA Concepción del Uruguay Fil: Rodriguez, Francisco I. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Procura, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Basavilbaso; Argentina 2019-12-06T11:43:25Z 2019-12-06T11:43:25Z 2018-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6462 0032-5791 1525-3171 https://doi.org/10.3382/ps/pey259 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Oxford Academic Press Poultry Science 97 (11) : 3826–3836 (November 2018) |
| spellingShingle | Enfermedades de los Animales Salmonella Serotipos Salmonella enteritidis Aves de Corral Heces Técnicas de Cultivo Animal Diseases Serotypes Poultry Faeces Culture Techniques Rodriguez, Francisco Procura, Francisco Bueno, Dante Javier Comparison of 7 culture methods for Salmonella serovar Enteritidis and Salmonella serovar Typhimurium isolation in poultry feces |
| title | Comparison of 7 culture methods for Salmonella serovar Enteritidis and Salmonella serovar Typhimurium isolation in poultry feces |
| title_full | Comparison of 7 culture methods for Salmonella serovar Enteritidis and Salmonella serovar Typhimurium isolation in poultry feces |
| title_fullStr | Comparison of 7 culture methods for Salmonella serovar Enteritidis and Salmonella serovar Typhimurium isolation in poultry feces |
| title_full_unstemmed | Comparison of 7 culture methods for Salmonella serovar Enteritidis and Salmonella serovar Typhimurium isolation in poultry feces |
| title_short | Comparison of 7 culture methods for Salmonella serovar Enteritidis and Salmonella serovar Typhimurium isolation in poultry feces |
| title_sort | comparison of 7 culture methods for salmonella serovar enteritidis and salmonella serovar typhimurium isolation in poultry feces |
| topic | Enfermedades de los Animales Salmonella Serotipos Salmonella enteritidis Aves de Corral Heces Técnicas de Cultivo Animal Diseases Serotypes Poultry Faeces Culture Techniques |
| url | http://hdl.handle.net/20.500.12123/6462 https://doi.org/10.3382/ps/pey259 |
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