Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vac...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S2405844019365338 http://hdl.handle.net/20.500.12123/6400 https://doi.org/10.1016/j.heliyon.2019.e02874 |
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| author | Serena, Maria Soledad Cappuccio, Javier Alejandro Metz, German Ernesto Aspitia, C.G. Dibarbora, Marina Gallo Calderon, Marina Beatriz Echeverria, Maria Gabriela |
| author_browse | Aspitia, C.G. Cappuccio, Javier Alejandro Dibarbora, Marina Echeverria, Maria Gabriela Gallo Calderon, Marina Beatriz Metz, German Ernesto Serena, Maria Soledad |
| author_facet | Serena, Maria Soledad Cappuccio, Javier Alejandro Metz, German Ernesto Aspitia, C.G. Dibarbora, Marina Gallo Calderon, Marina Beatriz Echeverria, Maria Gabriela |
| author_sort | Serena, Maria Soledad |
| collection | INTA Digital |
| description | Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms. |
| format | Artículo |
| id | INTA6400 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA64002019-11-26T14:41:10Z Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina Serena, Maria Soledad Cappuccio, Javier Alejandro Metz, German Ernesto Aspitia, C.G. Dibarbora, Marina Gallo Calderon, Marina Beatriz Echeverria, Maria Gabriela Cerdo Enfermedades de los Animales Parvovirus Porcino Cerdas Biotecnología Inmunología Swine Animal Diseases Porcine Parvovirus Sows Biotechnology Immunology Argentina Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms. EEA Marcos Juárez Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Metz, German Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Aspitia, C.G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Fil: Dibarbora, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Fil: Gallo Calderon, Marina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina Fil: Echeverria, Maria Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-11-26T14:38:12Z 2019-11-26T14:38:12Z 2019-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S2405844019365338 http://hdl.handle.net/20.500.12123/6400 2405-8440 https://doi.org/10.1016/j.heliyon.2019.e02874 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Heliyon 5 (11) : e02874 (November 2019) |
| spellingShingle | Cerdo Enfermedades de los Animales Parvovirus Porcino Cerdas Biotecnología Inmunología Swine Animal Diseases Porcine Parvovirus Sows Biotechnology Immunology Argentina Serena, Maria Soledad Cappuccio, Javier Alejandro Metz, German Ernesto Aspitia, C.G. Dibarbora, Marina Gallo Calderon, Marina Beatriz Echeverria, Maria Gabriela Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina |
| title | Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina |
| title_full | Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina |
| title_fullStr | Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina |
| title_full_unstemmed | Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina |
| title_short | Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina |
| title_sort | detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in argentina |
| topic | Cerdo Enfermedades de los Animales Parvovirus Porcino Cerdas Biotecnología Inmunología Swine Animal Diseases Porcine Parvovirus Sows Biotechnology Immunology Argentina |
| url | https://www.sciencedirect.com/science/article/pii/S2405844019365338 http://hdl.handle.net/20.500.12123/6400 https://doi.org/10.1016/j.heliyon.2019.e02874 |
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