In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions
Garlic blue mold disease, caused by Penicillium allii, is responsible for important economic losses produced yearly in garlic field crops and in postharvest storage. The identification of genetic resistance sources may help in the management of this disease. The present study investigated in vivo an...
| Autores principales: | , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2019
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| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007/s10658-019-01875-z http://hdl.handle.net/20.500.12123/6396 https://doi.org/10.1007/s10658-019-01875-z |
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| author | Salinas Palmieri, Maria Cecilia Cavagnaro, Pablo |
| author_browse | Cavagnaro, Pablo Salinas Palmieri, Maria Cecilia |
| author_facet | Salinas Palmieri, Maria Cecilia Cavagnaro, Pablo |
| author_sort | Salinas Palmieri, Maria Cecilia |
| collection | INTA Digital |
| description | Garlic blue mold disease, caused by Penicillium allii, is responsible for important economic losses produced yearly in garlic field crops and in postharvest storage. The identification of genetic resistance sources may help in the management of this disease. The present study investigated in vivo and in vitro antifungal effects of a genetically-diverse garlic collection against P. allii in two growing seasons. Cloves of garlic accessions were inoculated with P. allii conidia, and lesion area and sporulation of the fungus in the clove were estimated as a means to characterize the accessions response to the infection. Significant and continuous variation was found among the accessions (p < 0.001), suggesting a quantitative mode of resistance against P. allii in this garlic collection. ‘Castaño’ and ‘Peteco’ were consistently the most resistant and susceptible genotypes, respectively. Culture media of the fungus supplemented with crude garlic extracts (CGEs) revealed significant inhibition of P. allii growth in vitro by all the garlic accessions, with fungal-growth inhibition being dose-dependent up to a concentration of 20–26% (v/v), beyond which the CGEs became fungicidal. These data indicate that garlic bulbs have antifungal compounds, which at high concentration can be fungicidal for P. allii. CGEs of ‘Castaño’ and a few other accessions were consistently the more inhibitory extracts. Total phenolics and pyruvate (an estimator of the total thiosulfinates content) concentration in the garlic bulbs was not associated with P. allii resistance in vivo, but their content in the CGEs correlated significantly and negatively with the fungal growth in vitro (r = −0.38 to −0.72), suggesting that these compounds have antifungal effect, but their content is not the main factor conditioning P. allii resistance in vivo. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6396 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA63962019-11-26T12:35:08Z In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions Salinas Palmieri, Maria Cecilia Cavagnaro, Pablo Ajo Experimentación in Vitro Experimentación in Vivo Penicillium Enfermedades de las Plantas Control de Enfermedades Resistencia a la Enfermedad Garlic In Vitro Experimentation In Vivo Experimentation Plant Diseases Disease Control Disease Resistance Penicillium allii Garlic blue mold disease, caused by Penicillium allii, is responsible for important economic losses produced yearly in garlic field crops and in postharvest storage. The identification of genetic resistance sources may help in the management of this disease. The present study investigated in vivo and in vitro antifungal effects of a genetically-diverse garlic collection against P. allii in two growing seasons. Cloves of garlic accessions were inoculated with P. allii conidia, and lesion area and sporulation of the fungus in the clove were estimated as a means to characterize the accessions response to the infection. Significant and continuous variation was found among the accessions (p < 0.001), suggesting a quantitative mode of resistance against P. allii in this garlic collection. ‘Castaño’ and ‘Peteco’ were consistently the most resistant and susceptible genotypes, respectively. Culture media of the fungus supplemented with crude garlic extracts (CGEs) revealed significant inhibition of P. allii growth in vitro by all the garlic accessions, with fungal-growth inhibition being dose-dependent up to a concentration of 20–26% (v/v), beyond which the CGEs became fungicidal. These data indicate that garlic bulbs have antifungal compounds, which at high concentration can be fungicidal for P. allii. CGEs of ‘Castaño’ and a few other accessions were consistently the more inhibitory extracts. Total phenolics and pyruvate (an estimator of the total thiosulfinates content) concentration in the garlic bulbs was not associated with P. allii resistance in vivo, but their content in the CGEs correlated significantly and negatively with the fungal growth in vitro (r = −0.38 to −0.72), suggesting that these compounds have antifungal effect, but their content is not the main factor conditioning P. allii resistance in vivo. EEA La Consulta Fil: Salinas Palmieri, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina 2019-11-26T12:32:41Z 2019-11-26T12:32:41Z 2019-11-14 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s10658-019-01875-z http://hdl.handle.net/20.500.12123/6396 0929-1873 1573-8469 https://doi.org/10.1007/s10658-019-01875-z eng info:eu-repo/semantics/restrictedAccess application/pdf Springer European Journal of Plant Pathology : 1-15 (First Online: 14 November 2019) |
| spellingShingle | Ajo Experimentación in Vitro Experimentación in Vivo Penicillium Enfermedades de las Plantas Control de Enfermedades Resistencia a la Enfermedad Garlic In Vitro Experimentation In Vivo Experimentation Plant Diseases Disease Control Disease Resistance Penicillium allii Salinas Palmieri, Maria Cecilia Cavagnaro, Pablo In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions |
| title | In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions |
| title_full | In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions |
| title_fullStr | In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions |
| title_full_unstemmed | In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions |
| title_short | In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions |
| title_sort | in vivo and in vitro screening for resistance against penicillium allii in garlic accessions |
| topic | Ajo Experimentación in Vitro Experimentación in Vivo Penicillium Enfermedades de las Plantas Control de Enfermedades Resistencia a la Enfermedad Garlic In Vitro Experimentation In Vivo Experimentation Plant Diseases Disease Control Disease Resistance Penicillium allii |
| url | https://link.springer.com/article/10.1007/s10658-019-01875-z http://hdl.handle.net/20.500.12123/6396 https://doi.org/10.1007/s10658-019-01875-z |
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