Adaptation of genetically monomorphic bacteria : evolution of copper resistance through multiple horizontal gene transfers of complex and versatile mobile genetic elements
Copper-based antimicrobial compounds are widely used to control plant bacterial pathogens. Pathogens have adapted in response to this selective pressure. Xanthomonas citri pv. citri, a major citrus pathogen causing Asiatic citrus canker, was first reported to carry plasmid-encoded copper resistance...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/638 http://onlinelibrary.wiley.com/doi/10.1111/mec.14007/epdf?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=www.google.com.ar&purchase_site_license=LICENSE_DENIED |
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| author | Richard, Damien Ravigné, V. Rieux, A. Facon, B. Boyer, Claudine Boyer, K. Grygiel, P. Javegny, S. Terville, M. Canteros, Blanca Isabel Robène, I. Vernière, Christian Chabirand, A. Lefeuvre, Pierre Pruvost, Olivier |
| author_browse | Boyer, Claudine Boyer, K. Canteros, Blanca Isabel Chabirand, A. Facon, B. Grygiel, P. Javegny, S. Lefeuvre, Pierre Pruvost, Olivier Ravigné, V. Richard, Damien Rieux, A. Robène, I. Terville, M. Vernière, Christian |
| author_facet | Richard, Damien Ravigné, V. Rieux, A. Facon, B. Boyer, Claudine Boyer, K. Grygiel, P. Javegny, S. Terville, M. Canteros, Blanca Isabel Robène, I. Vernière, Christian Chabirand, A. Lefeuvre, Pierre Pruvost, Olivier |
| author_sort | Richard, Damien |
| collection | INTA Digital |
| description | Copper-based antimicrobial compounds are widely used to control plant bacterial pathogens. Pathogens have adapted in response to this selective pressure. Xanthomonas citri pv. citri, a major citrus pathogen causing Asiatic citrus canker, was first reported to carry plasmid-encoded copper resistance in Argentina. This phenotype was conferred by the copLAB gene system. The emergence of resistant strains has since been reported in R_eunion and Martinique. Using microsatellite-based genotyping and copLAB PCR, we demonstrated that the genetic structure of the copper-resistant strains from these three regions was made up of two distant clusters and varied for the detection of copLAB amplicons. In order to investigate this pattern more closely, we sequenced six copper-resistant X. citri pv. citri strains from Argentina, Martinique and R_eunion, together with reference copper-resistant Xanthomonas and Stenotrophomonas strains using long-read sequencing technology. Genes involved in copper resistance were found to be strain dependent with the novel identification in X. citri pv. citri of copABCD and a cus heavy metal efflux resistance–nodulation–division system. The genes providing the adaptive trait were part of a mobile genetic element similar to Tn3-like transposons and included in a conjugative plasmid. This indicates the system’s great versatility. The mining of all available bacterial genomes suggested that, within the bacterial community, the spread of copper resistance associated with mobile elements and their plasmid environments was primarily restricted to the Xanthomonadaceae family. |
| format | Artículo |
| id | INTA638 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA6382019-03-22T12:12:25Z Adaptation of genetically monomorphic bacteria : evolution of copper resistance through multiple horizontal gene transfers of complex and versatile mobile genetic elements Richard, Damien Ravigné, V. Rieux, A. Facon, B. Boyer, Claudine Boyer, K. Grygiel, P. Javegny, S. Terville, M. Canteros, Blanca Isabel Robène, I. Vernière, Christian Chabirand, A. Lefeuvre, Pierre Pruvost, Olivier Enfermedades de las Plantas Genética Resistencia a la Enfermedad Cobre Plant Diseases Genetics Disease Resistance Copper Copper-based antimicrobial compounds are widely used to control plant bacterial pathogens. Pathogens have adapted in response to this selective pressure. Xanthomonas citri pv. citri, a major citrus pathogen causing Asiatic citrus canker, was first reported to carry plasmid-encoded copper resistance in Argentina. This phenotype was conferred by the copLAB gene system. The emergence of resistant strains has since been reported in R_eunion and Martinique. Using microsatellite-based genotyping and copLAB PCR, we demonstrated that the genetic structure of the copper-resistant strains from these three regions was made up of two distant clusters and varied for the detection of copLAB amplicons. In order to investigate this pattern more closely, we sequenced six copper-resistant X. citri pv. citri strains from Argentina, Martinique and R_eunion, together with reference copper-resistant Xanthomonas and Stenotrophomonas strains using long-read sequencing technology. Genes involved in copper resistance were found to be strain dependent with the novel identification in X. citri pv. citri of copABCD and a cus heavy metal efflux resistance–nodulation–division system. The genes providing the adaptive trait were part of a mobile genetic element similar to Tn3-like transposons and included in a conjugative plasmid. This indicates the system’s great versatility. The mining of all available bacterial genomes suggested that, within the bacterial community, the spread of copper resistance associated with mobile elements and their plasmid environments was primarily restricted to the Xanthomonadaceae family. EEA Bella Vista Fil: Richard, Damien. CIRAD, UMR PVBMT; Francia. ANSES, Plant Health Laboratory; Francia. Université de la Réunion; Francia Fil: Ravigné, V. CIRAD, UMR PVBMT; Francia Fil: Rieux, A. CIRAD, UMR PVBMT; Francia Fil: Facon, B. Institut Nationale de la Recherche Agronomique; Francia Fil: Boyer, Claudine. CIRAD, UMR PVBMT; Francia Fil: Boyer, K. CIRAD, UMR PVBMT; Francia Fil: Grygiel, P. CIRAD, UMR PVBMT; Francia Fil: Javegny, S. CIRAD, UMR PVBMT; Francia Fil: Terville, M. CIRAD, UMR PVBMT; Francia Fil: Canteros, Blanca Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina Fil: Robène, I. CIRAD, UMR PVBMT; Francia Fil: Vernière, Christian. CIRAD, UMR PVBMT; Francia. CIRAD, UMR BGPI, Montpellier, Francia Fil: Chabirand, A. ANSES, Plant Health Laboratory; Francia Fil: Pruvost, Olivier. CIRAD, UMR PVBMT; Francia Fil: Lefeuvre, Pierre. CIRAD, UMR PVBMT; Francia 2017-07-11T15:10:23Z 2017-07-11T15:10:23Z 2017-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/638 http://onlinelibrary.wiley.com/doi/10.1111/mec.14007/epdf?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=www.google.com.ar&purchase_site_license=LICENSE_DENIED 1365-294X eng info:eu-repo/semantics/restrictedAccess application/pdf Molecular ecology 26 (7) : 2131-2149. (2017) |
| spellingShingle | Enfermedades de las Plantas Genética Resistencia a la Enfermedad Cobre Plant Diseases Genetics Disease Resistance Copper Richard, Damien Ravigné, V. Rieux, A. Facon, B. Boyer, Claudine Boyer, K. Grygiel, P. Javegny, S. Terville, M. Canteros, Blanca Isabel Robène, I. Vernière, Christian Chabirand, A. Lefeuvre, Pierre Pruvost, Olivier Adaptation of genetically monomorphic bacteria : evolution of copper resistance through multiple horizontal gene transfers of complex and versatile mobile genetic elements |
| title | Adaptation of genetically monomorphic bacteria : evolution of copper resistance through multiple horizontal gene transfers of complex and versatile mobile genetic elements |
| title_full | Adaptation of genetically monomorphic bacteria : evolution of copper resistance through multiple horizontal gene transfers of complex and versatile mobile genetic elements |
| title_fullStr | Adaptation of genetically monomorphic bacteria : evolution of copper resistance through multiple horizontal gene transfers of complex and versatile mobile genetic elements |
| title_full_unstemmed | Adaptation of genetically monomorphic bacteria : evolution of copper resistance through multiple horizontal gene transfers of complex and versatile mobile genetic elements |
| title_short | Adaptation of genetically monomorphic bacteria : evolution of copper resistance through multiple horizontal gene transfers of complex and versatile mobile genetic elements |
| title_sort | adaptation of genetically monomorphic bacteria evolution of copper resistance through multiple horizontal gene transfers of complex and versatile mobile genetic elements |
| topic | Enfermedades de las Plantas Genética Resistencia a la Enfermedad Cobre Plant Diseases Genetics Disease Resistance Copper |
| url | http://hdl.handle.net/20.500.12123/638 http://onlinelibrary.wiley.com/doi/10.1111/mec.14007/epdf?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=www.google.com.ar&purchase_site_license=LICENSE_DENIED |
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