Detection of bovine leukemia virus RNA in blood samples of naturally infected dairy cattle
The viral expression in vivo, in bovine leukemia virus (BLV)-infected cattle, is considered to be restricted to extremely low levels, and the mitosis of infected B lymphocytes is regarded as the main mode of virus persistence within the infected host. In this study, the presence of BLV RNA in whole...
| Autores principales: | , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/6315 https://www.mdpi.com/2306-7381/6/3/66 https://doi.org/10.3390/vetsci6030066 |
| _version_ | 1855035653568331776 |
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| author | Alvarez, Irene Porta, Natalia Gabriela Trono, Karina Gabriela |
| author_browse | Alvarez, Irene Porta, Natalia Gabriela Trono, Karina Gabriela |
| author_facet | Alvarez, Irene Porta, Natalia Gabriela Trono, Karina Gabriela |
| author_sort | Alvarez, Irene |
| collection | INTA Digital |
| description | The viral expression in vivo, in bovine leukemia virus (BLV)-infected cattle, is considered to be restricted to extremely low levels, and the mitosis of infected B lymphocytes is regarded as the main mode of virus persistence within the infected host. In this study, the presence of BLV RNA in whole blood from seven asymptomatic cows naturally infected with BLV during one year, including a complete milking cycle and two delivery time points, was investigated by nested-PCR using the oligonucleotides complementary to the tax and pol gene. BLV RNA was detected in four cows at different time points, especially in high blood proviral load cows and around delivery time. This study describes for the first time the detection of free BLV RNA in blood from BLV-infected asymptomatic cows. The results obtained suggest the occurrence of persistent low-level expression of the tax and pol genes that could be a result of viral reactivation, within the asymptomatic period. This finding may be important in the pathogenesis of BLV infection, associated with the delivery period. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6315 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA63152025-07-10T11:48:53Z Detection of bovine leukemia virus RNA in blood samples of naturally infected dairy cattle Alvarez, Irene Porta, Natalia Gabriela Trono, Karina Gabriela Bovine Leukaemia Virus Blood Sampling Dairy Cattle RNA Viruses Virus Leucemia Bovina Muestreo Sanguíneo Ganado de Leche The viral expression in vivo, in bovine leukemia virus (BLV)-infected cattle, is considered to be restricted to extremely low levels, and the mitosis of infected B lymphocytes is regarded as the main mode of virus persistence within the infected host. In this study, the presence of BLV RNA in whole blood from seven asymptomatic cows naturally infected with BLV during one year, including a complete milking cycle and two delivery time points, was investigated by nested-PCR using the oligonucleotides complementary to the tax and pol gene. BLV RNA was detected in four cows at different time points, especially in high blood proviral load cows and around delivery time. This study describes for the first time the detection of free BLV RNA in blood from BLV-infected asymptomatic cows. The results obtained suggest the occurrence of persistent low-level expression of the tax and pol genes that could be a result of viral reactivation, within the asymptomatic period. This finding may be important in the pathogenesis of BLV infection, associated with the delivery period. Instituto de Virología Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Porta, Natalia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-11-07T18:34:14Z 2019-11-07T18:34:14Z 2019-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6315 https://www.mdpi.com/2306-7381/6/3/66 2306-7381 https://doi.org/10.3390/vetsci6030066 eng info:eu-repograntAgreement/INTA/PNSA/1115054/AR./Enfermedades parasitarias, infecciosas y tóxico metabólicas que afectan la productividad de los bóvidos para producción de carne y leche. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Veterinary sciences 6 (3) : 66. (Septiembre 2019) |
| spellingShingle | Bovine Leukaemia Virus Blood Sampling Dairy Cattle RNA Viruses Virus Leucemia Bovina Muestreo Sanguíneo Ganado de Leche Alvarez, Irene Porta, Natalia Gabriela Trono, Karina Gabriela Detection of bovine leukemia virus RNA in blood samples of naturally infected dairy cattle |
| title | Detection of bovine leukemia virus RNA in blood samples of naturally infected dairy cattle |
| title_full | Detection of bovine leukemia virus RNA in blood samples of naturally infected dairy cattle |
| title_fullStr | Detection of bovine leukemia virus RNA in blood samples of naturally infected dairy cattle |
| title_full_unstemmed | Detection of bovine leukemia virus RNA in blood samples of naturally infected dairy cattle |
| title_short | Detection of bovine leukemia virus RNA in blood samples of naturally infected dairy cattle |
| title_sort | detection of bovine leukemia virus rna in blood samples of naturally infected dairy cattle |
| topic | Bovine Leukaemia Virus Blood Sampling Dairy Cattle RNA Viruses Virus Leucemia Bovina Muestreo Sanguíneo Ganado de Leche |
| url | http://hdl.handle.net/20.500.12123/6315 https://www.mdpi.com/2306-7381/6/3/66 https://doi.org/10.3390/vetsci6030066 |
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