Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoc...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Brazilian Association of Veterinary Pathology
2019
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| Materias: | |
| Acceso en línea: | https://bjvp.org.br/wp-content/uploads/2019/07/v12-n2-1.pdf http://hdl.handle.net/20.500.12123/6311 |
| _version_ | 1855483787841896448 |
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| author | Jauregui, Gloria R. Fontana, Diego Micheloud, Juan Francisco Prieto, Claudio Delgado, Fernando Oscar |
| author_browse | Delgado, Fernando Oscar Fontana, Diego Jauregui, Gloria R. Micheloud, Juan Francisco Prieto, Claudio |
| author_facet | Jauregui, Gloria R. Fontana, Diego Micheloud, Juan Francisco Prieto, Claudio Delgado, Fernando Oscar |
| author_sort | Jauregui, Gloria R. |
| collection | INTA Digital |
| description | Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed. |
| format | Artículo |
| id | INTA6311 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Brazilian Association of Veterinary Pathology |
| publisherStr | Brazilian Association of Veterinary Pathology |
| record_format | dspace |
| spelling | INTA63112019-11-07T17:37:43Z Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples Jauregui, Gloria R. Fontana, Diego Micheloud, Juan Francisco Prieto, Claudio Delgado, Fernando Oscar Rabies Virus Immunohistochemistry Diagnostic Techniques Cattle Virus de la Rabia Inmunohistoquímica Técnicas de Diagnosis Ganado Bovino Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed. Instituto de Patobiología Fil: Jauregui, Gloria R. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fontana, Diego. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta. Grupo de Trabajo de Patología, Epidemiología e Investigación Diagnóstica. Área de Sanidad Animal; Argentina Fil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Universidad del Salvador. Carrera de Veterinaria; Argentina 2019-11-07T17:18:37Z 2019-11-07T17:18:37Z 2019-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://bjvp.org.br/wp-content/uploads/2019/07/v12-n2-1.pdf http://hdl.handle.net/20.500.12123/6311 1983-0246 eng info:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Brazilian Association of Veterinary Pathology Brazilian journal of veterinary pathology 12 (2) : 27–32.(Julio 2019) |
| spellingShingle | Rabies Virus Immunohistochemistry Diagnostic Techniques Cattle Virus de la Rabia Inmunohistoquímica Técnicas de Diagnosis Ganado Bovino Jauregui, Gloria R. Fontana, Diego Micheloud, Juan Francisco Prieto, Claudio Delgado, Fernando Oscar Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples |
| title | Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples |
| title_full | Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples |
| title_fullStr | Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples |
| title_full_unstemmed | Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples |
| title_short | Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples |
| title_sort | evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples |
| topic | Rabies Virus Immunohistochemistry Diagnostic Techniques Cattle Virus de la Rabia Inmunohistoquímica Técnicas de Diagnosis Ganado Bovino |
| url | https://bjvp.org.br/wp-content/uploads/2019/07/v12-n2-1.pdf http://hdl.handle.net/20.500.12123/6311 |
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