Enzyme-linked immunosorbent assay as complement of intradermal skin test for the detection of mycobacterium bovis infection in cattle
Diagnostic tests based on cell-mediated immunity are used in programs for the control and eradication of bovine tuberculosis (bTB), which is mainly caused by Mycobacterium bovis. Additional serological assays could be performed as an ancillary method to detect an infected animal that fails to produc...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S1472979218303512 http://hdl.handle.net/20.500.12123/6307 https://doi.org/10.1016/j.tube.2019.05.006 |
| _version_ | 1855035652467326976 |
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| author | Garbaccio, Sergio Gabriel Garro, Carlos Javier Delgado, Fernando Oscar Tejada, G. A. Eirin, Maria Emilia Huertas, Pablo Sebastian Leon, Emilio Arnaldo Zumarraga, Martin Jose |
| author_browse | Delgado, Fernando Oscar Eirin, Maria Emilia Garbaccio, Sergio Gabriel Garro, Carlos Javier Huertas, Pablo Sebastian Leon, Emilio Arnaldo Tejada, G. A. Zumarraga, Martin Jose |
| author_facet | Garbaccio, Sergio Gabriel Garro, Carlos Javier Delgado, Fernando Oscar Tejada, G. A. Eirin, Maria Emilia Huertas, Pablo Sebastian Leon, Emilio Arnaldo Zumarraga, Martin Jose |
| author_sort | Garbaccio, Sergio Gabriel |
| collection | INTA Digital |
| description | Diagnostic tests based on cell-mediated immunity are used in programs for the control and eradication of bovine tuberculosis (bTB), which is mainly caused by Mycobacterium bovis. Additional serological assays could be performed as an ancillary method to detect an infected animal that fails to produce an immune response against the intradermal reaction (IDR), the official bTB test. In this study, we evaluated the effectiveness of an enzyme-linked immunosorbent assay (ELISA) that uses bovine PPD as a capture antigen as a complement to the IDR in herds with confirmed cases of bTB. The study was conducted in two stages. First, a panel of 200 serum samples was analyzed by ELISA. The sensitivity and specificity obtained were 60% and 99%, respectively. The subsequent stage consisted of evaluating 7,494 bovines from 14 selected dairy farms. The number of animals yielding a IDR negative/ELISA positive result were 200. A necropsy analysis of 33 of these IDR negative/ELISA positive animals revealed that 30 (91%) presented granulomatous lesions and positive M. bovis isolation. This finding confirmed bTB in most cases. Altogether, the results obtained in the present study suggest that the combined use of IDR and ELISA is an effective strategy to improve the control of bTB in endemic herds. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6307 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA63072019-11-07T14:16:19Z Enzyme-linked immunosorbent assay as complement of intradermal skin test for the detection of mycobacterium bovis infection in cattle Garbaccio, Sergio Gabriel Garro, Carlos Javier Delgado, Fernando Oscar Tejada, G. A. Eirin, Maria Emilia Huertas, Pablo Sebastian Leon, Emilio Arnaldo Zumarraga, Martin Jose Mycobacterium Bovis Infections Skin Tests Cattle Infección por Mycobacterium Bovis ELISA Tuberculosis Pruebas Cutáneas Ganado Bovino Diagnostic tests based on cell-mediated immunity are used in programs for the control and eradication of bovine tuberculosis (bTB), which is mainly caused by Mycobacterium bovis. Additional serological assays could be performed as an ancillary method to detect an infected animal that fails to produce an immune response against the intradermal reaction (IDR), the official bTB test. In this study, we evaluated the effectiveness of an enzyme-linked immunosorbent assay (ELISA) that uses bovine PPD as a capture antigen as a complement to the IDR in herds with confirmed cases of bTB. The study was conducted in two stages. First, a panel of 200 serum samples was analyzed by ELISA. The sensitivity and specificity obtained were 60% and 99%, respectively. The subsequent stage consisted of evaluating 7,494 bovines from 14 selected dairy farms. The number of animals yielding a IDR negative/ELISA positive result were 200. A necropsy analysis of 33 of these IDR negative/ELISA positive animals revealed that 30 (91%) presented granulomatous lesions and positive M. bovis isolation. This finding confirmed bTB in most cases. Altogether, the results obtained in the present study suggest that the combined use of IDR and ELISA is an effective strategy to improve the control of bTB in endemic herds. Instituto de Patobiología Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Tejada, G. A. Colón Diagnosis Laboratory Services; Argentina Fil: Eirin, Maria Emilia.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Huertas, Pablo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Leon, Emilio Arnaldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-11-07T14:08:34Z 2019-11-07T14:08:34Z 2019-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S1472979218303512 http://hdl.handle.net/20.500.12123/6307 1472-9792 https://doi.org/10.1016/j.tube.2019.05.006 eng info:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Tuberculosis 117: 56–61. (Julio 2019) |
| spellingShingle | Mycobacterium Bovis Infections Skin Tests Cattle Infección por Mycobacterium Bovis ELISA Tuberculosis Pruebas Cutáneas Ganado Bovino Garbaccio, Sergio Gabriel Garro, Carlos Javier Delgado, Fernando Oscar Tejada, G. A. Eirin, Maria Emilia Huertas, Pablo Sebastian Leon, Emilio Arnaldo Zumarraga, Martin Jose Enzyme-linked immunosorbent assay as complement of intradermal skin test for the detection of mycobacterium bovis infection in cattle |
| title | Enzyme-linked immunosorbent assay as complement of intradermal skin test for the detection of mycobacterium bovis infection in cattle |
| title_full | Enzyme-linked immunosorbent assay as complement of intradermal skin test for the detection of mycobacterium bovis infection in cattle |
| title_fullStr | Enzyme-linked immunosorbent assay as complement of intradermal skin test for the detection of mycobacterium bovis infection in cattle |
| title_full_unstemmed | Enzyme-linked immunosorbent assay as complement of intradermal skin test for the detection of mycobacterium bovis infection in cattle |
| title_short | Enzyme-linked immunosorbent assay as complement of intradermal skin test for the detection of mycobacterium bovis infection in cattle |
| title_sort | enzyme linked immunosorbent assay as complement of intradermal skin test for the detection of mycobacterium bovis infection in cattle |
| topic | Mycobacterium Bovis Infections Skin Tests Cattle Infección por Mycobacterium Bovis ELISA Tuberculosis Pruebas Cutáneas Ganado Bovino |
| url | https://www.sciencedirect.com/science/article/pii/S1472979218303512 http://hdl.handle.net/20.500.12123/6307 https://doi.org/10.1016/j.tube.2019.05.006 |
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