Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high a...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/630 https://www.nature.com/articles/s41598-017-00896-0 |
| _version_ | 1855482757159845888 |
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| author | Kurth, Daniel German Amadio, Ariel F. Ordoñez, Omar F. Allbarracín, Virginia H. Farías, María E. Gärtner, Wolfgang |
| author_browse | Allbarracín, Virginia H. Amadio, Ariel F. Farías, María E. Gärtner, Wolfgang Kurth, Daniel German Ordoñez, Omar F. |
| author_facet | Kurth, Daniel German Amadio, Ariel F. Ordoñez, Omar F. Allbarracín, Virginia H. Farías, María E. Gärtner, Wolfgang |
| author_sort | Kurth, Daniel German |
| collection | INTA Digital |
| description | Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high arsenic content, among others. After Socompa’s stromatolite microbial communities were analysed by metagenomic DNA sequencing, taxonomic classification showed dominance of Proteobacteria, Bacteroidetes and Firmicutes, and a remarkably high number of unclassified sequences. A functional analysis indicated that carbon fixation might occur not only by the Calvin-Benson cycle, but also through alternative pathways such as the reverse TCA cycle, and the reductive acetyl-CoA pathway. Deltaproteobacteria were involved both in sulfate reduction and nitrogen fixation. Significant differences were found when comparing the Socompa stromatolite metagenome to the Shark Bay (Australia) smooth mat metagenome: namely, those involving stress related processes, particularly, arsenic resistance. An in-depth analysis revealed a surprisingly diverse metabolism comprising all known types of As resistance and energy generating pathways. While the ars operon was the main mechanism, an important abundance of arsM genes was observed in selected phyla. The data resulting from this work will prove a cornerstone for further studies on this rare microbial community. |
| format | Artículo |
| id | INTA630 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA6302018-01-11T13:56:01Z Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis Kurth, Daniel German Amadio, Ariel F. Ordoñez, Omar F. Allbarracín, Virginia H. Farías, María E. Gärtner, Wolfgang Genética Arsénico Genómica Arsenic Genomics Genetics Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high arsenic content, among others. After Socompa’s stromatolite microbial communities were analysed by metagenomic DNA sequencing, taxonomic classification showed dominance of Proteobacteria, Bacteroidetes and Firmicutes, and a remarkably high number of unclassified sequences. A functional analysis indicated that carbon fixation might occur not only by the Calvin-Benson cycle, but also through alternative pathways such as the reverse TCA cycle, and the reductive acetyl-CoA pathway. Deltaproteobacteria were involved both in sulfate reduction and nitrogen fixation. Significant differences were found when comparing the Socompa stromatolite metagenome to the Shark Bay (Australia) smooth mat metagenome: namely, those involving stress related processes, particularly, arsenic resistance. An in-depth analysis revealed a surprisingly diverse metabolism comprising all known types of As resistance and energy generating pathways. While the ars operon was the main mechanism, an important abundance of arsM genes was observed in selected phyla. The data resulting from this work will prove a cornerstone for further studies on this rare microbial community. EEA Rafaela Fil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Amadio, Ariel F. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ordoñez, Omar F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Allbarracín, Virginia H. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales. Instituto Miguel Lillo; Argentina Fil: Farías, María E. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Gärtner, Wolfgang. Max-Planck Institute for Chemical Energy Conversion; Alemania 2017-07-10T18:56:51Z 2017-07-10T18:56:51Z 2017 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/630 https://www.nature.com/articles/s41598-017-00896-0 2045-2322 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Scientific Reports 7, Article number: 1024 |
| spellingShingle | Genética Arsénico Genómica Arsenic Genomics Genetics Kurth, Daniel German Amadio, Ariel F. Ordoñez, Omar F. Allbarracín, Virginia H. Farías, María E. Gärtner, Wolfgang Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis |
| title | Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis |
| title_full | Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis |
| title_fullStr | Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis |
| title_full_unstemmed | Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis |
| title_short | Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis |
| title_sort | arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis |
| topic | Genética Arsénico Genómica Arsenic Genomics Genetics |
| url | http://hdl.handle.net/20.500.12123/630 https://www.nature.com/articles/s41598-017-00896-0 |
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